Double-antibody method and the protein-A-bearing Staphylococcus aureus cells method compared for separating bound and free antigen in radioimmunoassay.

1979 ◽  
Vol 25 (5) ◽  
pp. 752-756 ◽  
Author(s):  
R K Gupta ◽  
D L Morton
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Human Albumin ◽  
Precipitation Method ◽  
High Adsorption ◽  
Time Saving ◽  
Double Antibody ◽  
Antibody Method ◽  
Free Antigen ◽  
Formalin Fixed

Abstract We compared the protein-A-bearing Staphylococcus aureus immunoadsorbent to the double-antibody precipitation method for separating bound and free radiolabeled antigen in a radioimmunoassay. With human albumin (antigen) and rabbit anti-human albumin (antibody) as a model, our results indicate that formalin-fixed, heat-killed S. aureus cells could be substituted for the double-antibody precipitation method. Ease of preparation and high adsorption capacity of protein-A-bearing S. aureus for most mammalian IgG make this method economical and time saving.

Solid-phase radioimmunoassay with protein-A-bearing Staphylococcus aureus cells used to assay a protein (ferritin) and a hapten (digoxin).

1980 ◽  
Vol 26 (1) ◽  
pp. 37-40
Author(s):  
J Gauldie ◽  
H K Tang ◽  
A Corsini ◽  
W H Walker
Keyword(s):  
Staphylococcus Aureus ◽  
Matrix Effects ◽  
Solid Phase ◽  
Protein A ◽  
General Applicability ◽  
Rabbit Igg ◽  
Human Sera ◽  
Solid Phase Radioimmunoassay ◽  
Formalin Fixed ◽  
Economical Alternative

Abstract We compared use of protein-A-containing Staphylococcus aureus bacteria with conventional ammonium sulfate precipitation and second-antibody methods of separating bound and free antigen in the radioimmunoassay of a hapten (digoxin) and protein (ferritin) in human sera. In each case, values obtained with the heat-killed, formalin-fixed bacteria correlated well with those found by established methods. No matrix effects were detected in either hapten or protein measurements. Because of the affinity of S. aureus for rabbit IgG, rabbit antisera could be used with a small number of bacteria to detect antigen in the presence of 50-fold excess human IgG. The availability of S. aureus and ease of handling make this reagent a rapid, economical alternative of general applicability in radioimmunoassay.

Solid-phase radioimmunoassay with protein-A-bearing Staphylococcus aureus cells used to assay a protein (ferritin) and a hapten (digoxin).

1980 ◽  
Vol 26 (1) ◽  
pp. 37-40 ◽  
Author(s):  
J Gauldie ◽  
H K Tang ◽  
A Corsini ◽  
W H Walker
Keyword(s):  
Staphylococcus Aureus ◽  
Matrix Effects ◽  
Solid Phase ◽  
Protein A ◽  
General Applicability ◽  
Rabbit Igg ◽  
Human Sera ◽  
Solid Phase Radioimmunoassay ◽  
Formalin Fixed ◽  
Economical Alternative

Abstract We compared use of protein-A-containing Staphylococcus aureus bacteria with conventional ammonium sulfate precipitation and second-antibody methods of separating bound and free antigen in the radioimmunoassay of a hapten (digoxin) and protein (ferritin) in human sera. In each case, values obtained with the heat-killed, formalin-fixed bacteria correlated well with those found by established methods. No matrix effects were detected in either hapten or protein measurements. Because of the affinity of S. aureus for rabbit IgG, rabbit antisera could be used with a small number of bacteria to detect antigen in the presence of 50-fold excess human IgG. The availability of S. aureus and ease of handling make this reagent a rapid, economical alternative of general applicability in radioimmunoassay.

Protein A-bearing Staphylococcus aureus as the solid phase in an enzyme immunoassay and its application to determination of urinary albumin.

1982 ◽  
Vol 28 (12) ◽  
pp. 2378-2382 ◽  
Author(s):  
J R Harper ◽  
N Mahmoudi ◽  
A Orengo
Keyword(s):  
Staphylococcus Aureus ◽  
Alkaline Phosphatase ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Protein A ◽  
Periodate Oxidation ◽  
Human Albumin ◽  
Urinary Albumin ◽  
Albumin Concentration ◽  
Sandwich Type

Abstract Protein A-bearing Staphylococcus aureus was used as a solid-phase matrix in a sandwich-type enzyme immunoassay for urinary albumin. Heat-inactivated, formalin-fixed bacteria were coated with affinity-purified goat anti-human albumin, exposed to solutions containing standard or unknown concentrations of albumin, then challenged with an alkaline phosphatase/anti-human albumin conjugate obtained by periodate oxidation. Alkaline phosphatase activity bound to the bacteria was a function of albumin concentration from 25 to 1000 micrograms/L. This assay was applied to determinations of urinary albumin concentrations between 1.25 and 1000 mg/L. Between-run CV was 2.55 (63.9 mg/L concentration). Within-run CVs for albumin concentrations of 1.9, 38.1, and 638.0 mg/L were 3.7, 3.7, and 2.4%, respectively. Analytical recovery was 95 to 107% across the full working range of the assay. Bence Jones proteins and hemoglobin had no significant effect on the assay. Nonspecific binding of the enzyme-antibody conjugate was 1.3% (SD = 0.7%). Values agreed well with those by radial immunodiffusion.

Development of a Radioimmunoassay for Pregnancy-Associated Plasma Protein A and Establishment of Normal Levels in the First Trimester of Pregnancy

1983 ◽  
Vol 20 (1) ◽  
pp. 26-30 ◽  
Author(s):  
F Anthony ◽  
G M Masson ◽  
P J Wood
Keyword(s):  
Plasma Protein ◽  
Protein A ◽  
First Trimester ◽  
Serum Samples ◽  
Double Antibody ◽  
Normal Ranges ◽  
Antibody Method ◽  
First Trimester Of Pregnancy

An accelerated double antibody method has been developed for the radioimmunoassay of pregnancy-associated plasma protein A (PAPP-A) in serum. The workable range for the assay was 0·04–1·8 mg/l of serum. PAPP-A levels were determined in single serum samples from 110 women with prospective normal pregnancies of between 7 and 14 weeks' gestation. The level of pregnancy specific β1 glycoprotein (SP1) was also measured in these samples and normal ranges for PAPP-A and SP1 were constructed from the results obtained.

Assay for islet cell antibodies. Protein A--monoclonal antibody method

Diabetes ◽  
1985 ◽  
Vol 34 (3) ◽  
pp. 300-305 ◽  
Author(s):  
S. Srikanta ◽  
A. Rabizadeh ◽  
M. A. Omar ◽  
G. S. Eisenbarth
Keyword(s):  
Monoclonal Antibody ◽  
Islet Cell ◽  
Protein A ◽  
Islet Cell Antibodies ◽  
Antibody Method

Detection of Rabies Virus and Its Antibodies Using Staphylococcus aureus Protein A

2015 ◽  
Vol 43 (1) ◽  
pp. 87-93
Author(s):  
Hemmat A ◽  
A Albehwar ◽  
M Shendy
Keyword(s):  
Staphylococcus Aureus ◽  
Rabies Virus ◽  
Protein A

scholarly journals Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 14
Author(s):  
Thamiris Santana Machado ◽  
Felipe Ramos Pinheiro ◽  
Lialyz Soares Pereira Andre ◽  
Renata Freire Alves Pereira ◽  
Reginaldo Fernandes Correa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Protein A ◽  
Invasive Infection ◽  
Type I ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Spa Gene ◽  
The City

Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection.

Formalin-Fixed Staphylococcus aureus Particles Prevent Allergic Sensitization in a Murine Model of Type I Allergy

2007 ◽  
Vol 144 (3) ◽  
pp. 183-196 ◽  
Author(s):  
Karina Gisch ◽  
Nadine Gehrke ◽  
Matthias Bros ◽  
Christina Priesmeyer ◽  
Jürgen Knop ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Murine Model ◽  
Allergic Sensitization ◽  
Type I ◽  
Type I Allergy ◽  
Formalin Fixed
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