Indirect evidence for the presence of creatine kinase isoenzyme BB in human heart tissue.

1982 ◽  
Vol 28 (4) ◽  
pp. 720-721 ◽  
Author(s):  
M Sheehan
Keyword(s):  
Creatine Kinase ◽  
Human Heart ◽  
Indirect Evidence ◽  
Heart Tissue ◽  
Creatine Kinase Isoenzyme ◽  
Human Heart Tissue

scholarly journals IMMUNOLOGIC RELATION OF STREPTOCOCCAL AND TISSUE ANTIGENS

1964 ◽  
Vol 119 (4) ◽  
pp. 651-666 ◽  
Author(s):  
Melvin H. Kaplan ◽  
Kathryn H. Svec
Keyword(s):  
Human Heart ◽  
Streptococcal Infection ◽  
Heart Tissue ◽  
Antigenic Determinants ◽  
Heat Inactivation ◽  
Prolonged Storage ◽  
Acute Glomerulonephritis ◽  
Streptococcal Antigen ◽  
Reactive Antibody ◽  
Human Heart Tissue

Sera from patients with recent streptococcal infection or non-suppurative sequelae exhibit with variable frequency a precipitin reaction in agar gel with a partially purified streptococcal antigen which has been shown previously to be immunologically related to human heart tissue. This precipitin could be absorbed from sera with normal human heart tissue homogenates but not with homogenates of other organs. Demonstration of this cross-reaction by heart absorption was found dependent both upon the serologic properties of individual sera and the nature or state of purification of the streptococcal product employed as test antigen. Evidence was obtained of a close association of heart-related and non-heart-related antigenic determinants in partially purified preparations of the streptococcal antigen by both gel diffusion and immunoelectrophoresis. On immunoelectrophoretic analysis, cross-reactive antigen exhibited a more rapid mobility toward the anode than M protein. It was destroyed by digestion with trypsin, pepsin, and chymotrypsin. Based on specific absorption tests with a Type 5 and Type 19 strain, the antigen was localized to cell walls and to a lesser extent to cell membranes of these strains. Precipitating activity related to cross-reactive antibody was localized to the immunoglobulin zone in immunoelectrophoresis. Reactive sera showed diminution or loss of serological activity following heat inactivation at 56°C or after prolonged storage at 4°C. Sera containing cross-reactive precipitating antibody exhibited an immunofluorescent reaction with sarcolemma of cardiac myofibers, which was inhibited by streptococcal cross-reactive antigen. By this inhibition test, the immunofluorescent reaction related to cross-reactive antibody could be distinguished from that due to other heart-reactive factors. Antibody to streptococcal cross-reactive antigen defined by precipitation-absorption tests was observed in 24 per cent of patients with recent history of uncomplicated streptococcal infection and in the majority of patients with acute rheumatic fever, rheumatic heart disease, or acute glomerulonephritis. It was observed rarely in patients with non-streptococcal related disease. These data provide evidence that induction of cross-reactive autoantibody to heart in certain individuals is associated with streptococcal infection.

scholarly journals Factors for C-Kit Expression in Cardiac Outgrowth Cells and Human Heart Tissue

2017 ◽  
Vol 58 (6) ◽  
pp. 962-968
Author(s):  
Satoshi Matsushita ◽  
Kazuo Minematsu ◽  
Taira Yamamoto ◽  
Hirotaka Inaba ◽  
Kenji Kuwaki ◽  
...  
Keyword(s):  
Human Heart ◽  
Heart Tissue ◽  
Human Heart Tissue

scholarly journals Chronotropic Responses of Human Heart Tissue Cultures

1972 ◽  
Vol 30 (6) ◽  
pp. 628-633 ◽  
Author(s):  
T. D. CHANG ◽  
G. R. CUMMING
Keyword(s):  
Human Heart ◽  
Heart Tissue ◽  
Tissue Cultures ◽  
Human Heart Tissue

Abstract 335: Titin Truncations in Human Heart Tissue

2019 ◽  
Vol 125 (Suppl_1) ◽  
Author(s):  
Quentin McAfee ◽  
Jeff Brandimarto ◽  
Joshua Rhoades ◽  
Ken Bede ◽  
Kenneth Margulies ◽  
...  
Keyword(s):  
Human Heart ◽  
Heart Tissue ◽  
Human Heart Tissue

scholarly journals Cardioprotective Effects of Glycyrrhiza uralensis Extract Against Doxorubicin- Induced Toxicity

2011 ◽  
Vol 30 (2) ◽  
pp. 181-189 ◽  
Author(s):  
Ling Zhang ◽  
Yinxin Yang ◽  
Lingyan Yu ◽  
Yi Wang ◽  
Li Liu ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Heart Tissue ◽  
Glycyrrhiza Uralensis ◽  
Creatine Kinase Isoenzyme ◽  
Imprinting Control Region ◽  
Cardioprotective Effects ◽  
Histopathologic Analysis

The purpose of the present study was to investigate the cardioprotective effects of Glycyrrhiza uralensis extract (GUE) against doxorubicin (DOX)-induced cardiotoxicity. Imprinting control region (ICR) mice were treated with saline, DOX (20 mg/kg intraperitoneal [ip] for once), GUE (100 mg/kg intragastric [ig] for 8 days), co-treatments with DOX and GUE (100 mg/kg ig for 8 days), and amifostine (100 mg/kg intravenous [iv] for once), respectively. Serum levels of lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB), glutathione peroxidase (GSH-PX) activity, and glutathione (GSH) level in heart tissue were measured. Histopathologic analysis of heart tissue was also performed. Treatment with GUE significantly protected the mice from DOX-induced cardiotoxicity, indicated by decreased levels of serum LDH and CK-MB, improved heart morphology and increased GSH-PX activity and GSH level. Additionally, GUE did not compromise the tumor-inhibitory effect of DOX. In conclusion, our studies imply the potentially clinical application of GUE to overcome the cardiotoxicity of doxorubicin.

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