Characterization of Hemoglobin Lepore Variants by Advanced Mass-Spectrometric Procedures

1992 ◽  
Vol 38 (8) ◽  
pp. 1444-1448 ◽  
Author(s):  
M De Caterina ◽  
P Esposito ◽  
E Grimaldi ◽  
G Di Maro ◽  
F Scopacasa ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Fast Atom Bombardment ◽  
Accurate Determination ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Direct Examination ◽  
Peptide Mixture ◽  
Analytical Protocol

Abstract We describe an analytical protocol for characterizing the molecular structure of hemoglobin (Hb) Lepore variants by using two different mass-spectrometric approaches. The first method consists of direct examination of the chromatographically separated hybrid globins by electro-spray mass spectrometry; the variant Lepore globin is identified through the accurate determination of its molecular mass. Alternatively, the anomalous globins are digested with trypsin and their structures are determined by fast atom bombardment mass-spectrometric analysis of the peptide mixture. The application of this procedure to the identification of Hb Lepore Boston and Hb Lepore Baltimore is described.

Fast Atom Bombardment Mass Spectrometric Analysis of Base Hydrolyzed γ-Lactone

1993 ◽  
Vol 76 (4) ◽  
pp. 913-917
Author(s):  
Randall J Baker
Keyword(s):  
Molecular Structure ◽  
Room Temperature ◽  
Fast Atom Bombardment ◽  
Ion Pairs ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Thermally Induced ◽  
Fab Mass Spectrometry ◽  
Hydroxy Carboxylic Acid

Abstract Fast atom bombardment (FAB) mass spectrometry (MS) is used to characterize the γ-hydroxy carboxylic acid (γ-HCa) formed from base hydrolyzed γ-lactone at room temperature. Acidification of this base hydrolyzed solution reconverts the γ-HCa back to γ-lactone. Mass shift differences between tetramethylammonium (TMA+) and Na+ ion-pairs with the anionic γ-HCarboxylate(–) (γ-HC(–)) are used to confirm molecular ion identifications. FAB ionization was required to provide mass characterization of the γ-HCa. Electron ionization (El) MS of the γ-HCa was unsuccessful and reconverted γ-HCa back to γ-lactone because of complete thermally induced dehydration. The present paper suggests structures for El fragmentation that support the molecular structure of γ-lactone.

Fast Atom Bombardment Mass Spectrometric Characterization of Poly(O-Toluidine)

1991 ◽  
Author(s):  
K. Balasaunmugam ◽  
K. G. Owens ◽  
K. F. Hsueh ◽  
P. Hoontrakul ◽  
M. A. Olsen
Keyword(s):  
Fast Atom Bombardment ◽  
Mass Spectrometric

scholarly journals Comparison of Elemental Analysis Techniques for the Characterization of Commercial Alloys

Metals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 736
Author(s):  
Peter Seidel ◽  
Doreen Ebert ◽  
Robert Schinke ◽  
Robert Möckel ◽  
Simone Raatz ◽  
...  
Keyword(s):  
Accurate Determination ◽  
Analytical Techniques ◽  
Optical Emission ◽  
Breakdown Spectroscopy ◽  
General Evaluation ◽  
Sample Composition ◽  
Laser Induced Breakdown ◽  
Copper Aluminum

Better quality control for alloy manufacturing and sorting of post-consumer scraps relies heavily on the accurate determination of their chemical composition. In recent decades, analytical techniques, such as X-ray fluorescence spectroscopy (XRF), laser-induced breakdown spectroscopy (LIBS), and spark optical emission spectroscopy (spark-OES), found widespread use in the metal industry, though only a few studies were published about the comparison of these techniques for commercially available alloys. Hence, we conducted a study on the evaluation of four analytical techniques (energy-dispersive XRF, wavelength-dispersive XRF, LIBS, and spark-OES) for the determination of metal sample composition. It focuses on the quantitative analysis of nine commercial alloys, representing the three most important alloy classes: copper, aluminum, and steel. First, spark-OES is proven to serve as a validation technique in the use of certified alloy reference samples. Following an examination of the lateral homogeneity by XRF, the results of the techniques are compared, and reasons for deviations are discussed. Finally, a more general evaluation of each technique with its capabilities and limitations is given, taking operation-relevant parameters, such as measurement speed and calibration effort, into account. This study shall serve as a guide for the routine use of these methods in metal producing and recycling industries.

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