scholarly journals Evaluation of a Capillary Electrophoresis Method for Routine Determination of Hemoglobins A2 and F

1999 ◽  
Vol 45 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Frédéric Cotton ◽  
Changying Lin ◽  
Bernard Fontaine ◽  
Béatrice Gulbis ◽  
Jacques Janssens ◽  
...  
Keyword(s):  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Hemoglobin F ◽  
Low Concentrations ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Hb S

Abstract Hemoglobin A2 (Hb A2) and hemoglobin F (Hb F) are important analytes in the diagnosis and follow up of Hb diseases. We evaluated a new capillary zone electrophoresis (CZE) kit for Hb A2 and Hb F measurements. The imprecision ranged from 3% to 6% for Hb A2 and Hb F at physiological and pathological concentrations. The method compared well with cation-exchange HPLC for Hb A2 and Hb F and with anion-exchange chromatography in microcolumns (MAEC), for Hb A2. Nevertheless, higher results were obtained [Hb A2 CZE (%) = 1.233 Hb A2 HPLC − 0.2; Hb A2 CZE (%) = 1.190 Hb A2 MAEC + 0.1; Hb FCZE (%) = 1.118 Hb FHPLC + 0.4], and new reference values had to be determined (Hb A2 2.7–3.8%; Hb F <1.2%). Quantification of Hb A2 was not influenced by Hb S. Measurement of Hb F was accurate and precise except at low concentrations in Hb AS patients. This new CZE kit is rapid, precise, and reliable, and seems appropriate for use in clinical laboratories.

scholarly journals Separation of Arsenic and Selenium Species by Capillary Zone Electrophoresis in a Coated Capillary

1999 ◽  
Vol 82 (6) ◽  
pp. 1587-1593 ◽  
Author(s):  
Natalia G Vanifatova ◽  
Boris Y Spivakov ◽  
Juergen Mattusch ◽  
Rainer Wennrich
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Sensitivity Enhancement ◽  
Selenium Species ◽  
Long Term Stability ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Matrix Removal

Abstract A capillary zone electrophoresis method was developed for the determination of 8 arsenic and selenium species in a polymer-coated capillary. Large-volume stacking with matrix removal was used for sensitivity enhancement. The entire analysis time was only a few minutes. The experimental sensitivity enhancement for 7 species was found to be near its theoretical value. Coating capillaries for long-term stability in alkaline buffer electrolytes was also investigated.

A fast and validated capillary zone electrophoresis method for the determination of selected fatty acids applied to food and cosmetic purposes

2019 ◽  
Vol 11 (43) ◽  
pp. 5607-5612 ◽  
Author(s):  
Tatiane Lima Amorim ◽  
Mariana Gavioli dos Reis Pena ◽  
Fabiano Freire Costa ◽  
Marcone Augusto Leal de Oliveira ◽  
Paula Rocha Chellini
Keyword(s):  
Fatty Acids ◽  
Capillary Electrophoresis ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Oleic And Linoleic Acids

The potential of capillary electrophoresis was demonstrated to the determination of fatty acids in food and cosmetic samples and a validated method is proposed to palmitic, stearic, oleic and linoleic acids quantification in these samples.

Determination of Vitamin C Content in Saposhnikovia Divaricata by Capillary Zone Electrophoresis

2014 ◽  
Vol 998-999 ◽  
pp. 224-227
Author(s):  
Hai Xing Liu ◽  
Xiao Ping Zhang ◽  
Quan Hua Fan ◽  
Yang Xu ◽  
Ting Ting Huang ◽  
...  
Keyword(s):  
Vitamin C ◽  
Capillary Zone Electrophoresis ◽  
Electrophoretic Analysis ◽  
Zone Electrophoresis ◽  
Borax Solution ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Saposhnikovia Divaricata ◽  
Acetonitrile Concentration

In this paper, using capillary zone electrophoresis method determined Vitamin C content in saposhnikovia divaricata. 20 mmol/L borax solution and 20% acetonitrile concentration, 20kV voltage, 254 nm UV detection wavelength was chosen for electrophoretic analysis. Measured vitamin C content in saposhnikovia divaricata were 0.297 mg/g (RSD=5.9%) (n=7).

Capillary zone electrophoresis method for the direct determination of amino acids in recombinant human erythropoietin preparations used for the treatment of anemia

2015 ◽  
Vol 36 (9-10) ◽  
pp. 1179-1185 ◽  
Author(s):  
Izabel Cristina de Souza Crespo ◽  
Matheus Troina de Resende ◽  
Annibal Duarte Pereira Netto ◽  
Flávia Ferreira de Carvalho Marques
Keyword(s):  
Amino Acids ◽  
Capillary Zone Electrophoresis ◽  
Recombinant Human Erythropoietin ◽  
Direct Determination ◽  
Human Erythropoietin ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone

Validation of a capillary zone electrophoresis method for the comparative determination of etoricoxib in pharmaceutical formulations

2008 ◽  
Vol 31 (1) ◽  
pp. 169-176 ◽  
Author(s):  
Sérgio Luiz Dalmora ◽  
Maximiliano da Silva Sangoi ◽  
Lucélia Magalhães da Silva ◽  
Rui Oliveira Macedo ◽  
Thiago Barth
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Pharmaceutical Formulations ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone ◽  
Comparative Determination

Very fast and direct capillary zone electrophoresis method for the determination of creatinine and creatine in human urine

2004 ◽  
Vol 521 (1) ◽  
pp. 53-59 ◽  
Author(s):  
J. Rodrı́guez ◽  
J.J. Berzas ◽  
G. Castañeda ◽  
N. Mora ◽  
M.J. Rodrı́guez
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Human Urine ◽  
Zone Electrophoresis ◽  
Electrophoresis Method ◽  
Capillary Zone
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