Paeoniae albaRadix Promotes Peripheral Nerve Regeneration

The present study providesin vitroandin vivoevaluation ofPaeoniae albaRadix (PR) on peripheral nerve regeneration. In thein vitrostudy, we found the PR caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from PC12 cells as well as their expression of growth associated protein 43 and synapsin I. In thein vivostudy, silicone rubber chambers filled with the PR water extract were used to bridge a 10-mm sciatic nerve defect in rats. At the conclusion of 8 weeks, regenerated nerves in the PR groups, especially at 1.25 mg ml−1had a higher rate of successful regeneration across the wide gap, relatively larger mean values of total nerve area, myelinated axon count and blood vessel number, and a significantly larger nerve conductive velocity compared to the control group (P < .05). These results suggest that the PR extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of injured peripheral nerve.

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Effect of Arecoline on Regeneration of Injured Peripheral Nerves

The American Journal of Chinese Medicine ◽

10.1142/s0192415x13500584 ◽

2013 ◽

Vol 41 (04) ◽

pp. 865-885 ◽

Cited By ~ 4

Author(s):

Sheng-Chi Lee ◽

Chin-Chuan Tsai ◽

Chun-Hsu Yao ◽

Yuan-Man Hsu ◽

Yueh-Sheng Chen ◽

...

Keyword(s):

Sciatic Nerve ◽

Peripheral Nerve ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

In Vitro Study ◽

Control Group ◽

Neural Cells ◽

In Vivo Evaluation

The present study provides in vitro and in vivo evaluation of arecoline on peripheral nerve regeneration. In the in vitro study, we found that arecoline at 50 μg/ml could significantly promote the survival and outgrowth of cultured Schwann cells as compared to the controls treated with culture medium only. In the in vivo study, we evaluated peripheral nerve regeneration across a 10-mm gap in the sciatic nerve of the rat, using a silicone rubber nerve chamber filled with the arecoline solution. In the control group, the chambers were filled with normal saline only. At the end of the fourth week, morphometric data revealed that the arecoline-treated group at 5 μg/ml significantly increased the number and the density of myelinated axons as compared to the controls. Immunohistochemical staining in the arecoline-treated animals at 5 μg/ml also showed their neural cells in the L4 and L5 dorsal root ganglia ipsilateral to the injury were strongly retrograde-labeled with fluorogold and lamina I–II regions in the dorsal horn ipsilateral to the injury were significantly calcitonin gene-related peptide-immunolabeled compared with the controls. In addition, we found that the number of macrophages recruited in the distal sciatic nerve was increased as the concentration of arecoline was increased. Electrophysiological measurements showed the arecoline-treated groups at 5 and 50 μg/ml had a relatively larger nerve conductive velocity of the evoked muscle action potentials compared to the controls. These results indicate that arecoline could stimulate local inflammatory conditions, improving the recovery of a severe peripheral nerve injury.

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Puerarin Accelerates Peripheral Nerve Regeneration

The American Journal of Chinese Medicine ◽

10.1142/s0192415x11009500 ◽

2011 ◽

Vol 39 (06) ◽

pp. 1207-1217 ◽

Cited By ~ 13

Author(s):

Shih-Wei Hsiang ◽

Han-Chung Lee ◽

Fuu-Jen Tsai ◽

Chin-Chuan Tsai ◽

Chun-Hsu Yao ◽

...

Keyword(s):

Peripheral Nerve ◽

Nerve Regeneration ◽

Culture Medium ◽

Peripheral Nerve Regeneration ◽

Control Group ◽

Cultured Schwann Cells ◽

Regeneration In Vitro ◽

Potential Area

This study investigates the effect of puerarin (PR) on peripheral nerve regeneration in vitro and in vivo. PR at concentrations of 1, 10, and 100 μM significantly promoted survival and outgrowth of cultured Schwann cells, as compared to the controls treated with culture medium only. in vivo study, peripheral nerve regeneration was evaluated across a 15-mm gap in the sciatic nerve of rats using a silicone rubber nerve chamber filled with PR solution. The control group chambers were filled with normal saline only. At the end of eight weeks, animals in the PR groups, especially at a concentration of 1 μM, had a significantly higher density of myelinated axons, greater evoked action potential area, and a larger nerve conductive velocity, as compared to the controls. All experimental results indicate that PR treatment promotes nerve growth and is a promising herbal medicine for recovery of regenerating peripheral nerves.

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