scholarly journals Community composition and assembly processes of the free-living and particle-attached bacteria in Taihu Lake

2017 ◽  
Vol 93 (6) ◽  
Author(s):  
Dayong Zhao ◽  
Huimin Xu ◽  
Jin Zeng ◽  
Xinyi Cao ◽  
Rui Huang ◽  
...  
Keyword(s):  
Community Composition ◽  
Taihu Lake ◽  
Free Living ◽  
Attached Bacteria

scholarly journals Diversity of Free-Living and Attached Bacteria in Offshore Western Mediterranean Waters as Depicted by Analysis of Genes Encoding 16S rRNA

1999 ◽  
Vol 65 (2) ◽  
pp. 514-522 ◽  
Author(s):  
Silvia G. Acinas ◽  
Josefa Antón ◽  
Francisco Rodríguez-Valera
Keyword(s):  
16S Rdna ◽  
Restriction Analysis ◽  
Western Mediterranean ◽  
Bacterial Cells ◽  
Free Living ◽  
Attached Bacteria ◽  
Genes Encoding ◽  
Community Fingerprinting ◽  
Close Relatives ◽  
Alteromonas Macleodii

ABSTRACT In a previous study (S. G. Acinas, F. Rodrı́guez-Valera, and C. Pedrós-Alió, FEMS Microbiol. Ecol. 24:27–40, 1997), community fingerprinting by 16S rDNA restriction analysis applied to Mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 μm. Here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16S rDNA obtained from the same samples, and we have also studied the samples by scanning electron microscopy to detect morphology patterns. As expected, the sequences retrieved from the assemblages were very different. The subsample of attached bacteria contained very little diversity, with close relatives of a well-known species of marine bacteria, Alteromonas macleodii, representing the vast majority of the clones at every depth. On the other hand, the free-living assemblage was highly diverse and varied with depth. At 400 m, close relatives of cultivated γProteobacteria predominated, but as shown by other authors, near the surface most clones were related to phylotypes described only by sequence, in which the α Proteobacteria of the SAR11 cluster predominated. The new technique of rDNA internal spacer analysis has been utilized, confirming these results. Clones representative of the A. macleodii cluster have been completely sequenced, producing a picture that fits well with the idea that they could represent a genus with at least two species and with a characteristic depth distribution.

The dynamics of attached and free-living bacterial population in tropical coastal waters

2015 ◽  
Vol 66 (8) ◽  
pp. 701 ◽  
Author(s):  
Siew Wen Lee ◽  
Choon Weng Lee ◽  
Chui Wei Bong ◽  
Kumaran Narayanan ◽  
Edmund Ui-Hang Sim
Keyword(s):  
Coastal Waters ◽  
Bacterial Population ◽  
Bacterial Abundance ◽  
Large Fraction ◽  
Free Living ◽  
Water Port ◽  
Eutrophic Water ◽  
Attached Bacteria ◽  
Student’S T ◽  
Student’S T Test

We investigated the dynamics of attached and free-living bacterial abundance over a period of 18 months in tropical coastal waters of Malaysia. We measured the abundance at both oligotrophic coastal water (Port Dickson) and eutrophic estuary (Klang), and hypothesised that attached bacteria are predominant in eutrophic waters. We found that bacterial abundance was higher at Klang than Port Dickson (Student’s t-test: t=4.87, d.f.=19, P<0.001). Attached bacteria also formed a large fraction of the total bacteria at Klang (75% ±13s.d.) relative to Port Dickson (56% ±22), and showed preference for chlorophyll-a-based particles rather than total suspended solids. The bacterial community structure was clearly different between the two stations but was similar between the attached and free-living bacterial population. Our results showed the importance of attached bacteria in eutrophic water where they could play a major role in carbon and nutrient cycling.

Biogeography of the free-living and particle-attached bacteria in Tibetan lakes

2019 ◽  
Vol 95 (7) ◽  
Author(s):  
Keshao Liu ◽  
Juzhi Hou ◽  
Yongqin Liu ◽  
Anyi Hu ◽  
Mingda Wang ◽  
...  
Keyword(s):  
Free Living ◽  
Attached Bacteria

scholarly journals Changes in Free-Living and Particle-Associated Bacterial Communities Depending on the Growth Phases of Marine Green Algae, Tetraselmis suecica

2021 ◽  
Vol 9 (2) ◽  
pp. 171
Author(s):  
Bum Soo Park ◽  
Won-Ji Choi ◽  
Ruoyu Guo ◽  
Hansol Kim ◽  
Jang-Seu Ki
Keyword(s):  
Bacterial Community ◽  
Community Composition ◽  
Green Algae ◽  
Growth Phase ◽  
Bacterial Communities ◽  
Algal Growth ◽  
Cell Number ◽  
Phase Lag ◽  
Tetraselmis Suecica ◽  
Free Living

Bacteria are remarkably associated with the growth of green algae Tetraselmis which are used as a feed source in aquaculture, but Tetraselmis-associated bacterial community is characterized insufficiently. Here, as a first step towards characterization of the associated bacteria, we investigated the community composition of free-living (FLB) and particle-associated (PAB) bacteria in each growth phase (lag, exponential, stationary, and death) of Tetraselmis suecica P039 culture using pyrosequencing. The percentage of shared operational taxonomic units (OTUs) between FLB and PAB communities was substantially high (≥92.4%), but their bacterial community compositions were significantly (p = 0.05) different from each other. The PAB community was more variable than the FLB community depending on the growth phase of T. suecica. In the PAB community, the proportions of Marinobacter and Flavobacteriaceae were considerably varied in accordance with the cell number of T. suecica, but there was no clear variation in the FLB community composition. This suggests that the PAB community may have a stronger association with the algal growth than the FLB community. Interestingly, irrespective of the growth phase, Roseobacter clade and genus Muricauda were predominant in both FLB and PAB communities, indicating that bacterial communities in T. suecica culture may positively affect the algae growth and that they are potentially capable of enhancing the T. suecica growth.

scholarly journals Diel Protein Regulation of Marine Picoplanktonic Communities Assessed by Metaproteomics

2021 ◽  
Vol 9 (12) ◽  
pp. 2621
Author(s):  
Augustin Géron ◽  
Johannes Werner ◽  
Philippe Lebaron ◽  
Ruddy Wattiez ◽  
Sabine Matallana-Surget
Keyword(s):  
Heterotrophic Bacteria ◽  
Temporal Structure ◽  
Sea Surface ◽  
Diel Cycle ◽  
Diel Variation ◽  
Protein Regulation ◽  
Free Living ◽  
Bacterial Populations ◽  
Form Complex ◽  
Attached Bacteria

The diel cycle is of enormous biological importance in that it imposes temporal structure on ecosystem productivity. In the world’s oceans, microorganisms form complex communities that carry out about half of photosynthesis and the bulk of life-sustaining nutrient cycling. How the functioning of microbial communities is impacted by day and night periods in surface seawater remains to be elucidated. In this study, we compared the day and night metaproteomes of the free-living and the particle-attached bacterial fractions from picoplanktonic communities sampled from the northwest Mediterranean Sea surface. Our results showed similar taxonomic distribution of free-living and particle-attached bacterial populations, with Alphaproteobacteria, Gammaproteobacteria and Cyanobacteria being the most active members. Comparison of the day and night metaproteomes revealed that free-living and particle-attached bacteria were more active during the day and the night, respectively. Interestingly, protein diel variations were observed in the photoautotroph Synechococcales and in (photo)-heterotrophic bacteria such as Flavobacteriales, Pelagibacterales and Rhodobacterales. Moreover, our data demonstrated that diel cycle impacts light-dependent processes such as photosynthesis and UV-stress response in Synechococcales and Rhodobacterales, respectively, while the protein regulation from the ubiquitous Pelagibacterales remained stable over time. This study unravels, for the first time, the diel variation in the protein expression of major free-living and particle-attached microbial players at the sea surface, totaling an analysis of eight metaproteomes.

The antibiotic resistome of free-living and particle-attached bacteria under a reservoir cyanobacterial bloom

2018 ◽  
Vol 117 ◽  
pp. 107-115 ◽  
Author(s):  
Yunyan Guo ◽  
Min Liu ◽  
Lemian Liu ◽  
Xuan Liu ◽  
Huihuang Chen ◽  
...  
Keyword(s):  
Cyanobacterial Bloom ◽  
Free Living ◽  
Attached Bacteria
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