scholarly journals Assessing the impact of a viral infection on the expression of transposable elements in the cabbage looper moth (Trichoplusia ni)

2021 ◽  
Author(s):  
Héloïse Muller ◽  
Vincent Loiseau ◽  
Sandra Guillier ◽  
Richard Cordaux ◽  
Clément Gilbert
Keyword(s):  
Transposable Element ◽  
Horizontal Transfer ◽  
Trichoplusia Ni ◽  
Expression Profiles ◽  
Large Fraction ◽  
Cabbage Looper ◽  
Genome Architecture ◽  
Cabbage Looper Moth ◽  
A Cell ◽  
The Impact

Abstract Most studies of stress-induced transposable element (TE) expression have so far focused on abiotic sources of stress. Here we analyzed the impact of an infection by the AcMNPV baculovirus on TE expression in a cell line (Tnms42) and midgut tissues of the cabbage looper moth (Trichoplusia ni). We find that a large fraction of TE families (576/636 in Tnms42 cells and 503/612 in midgut) is lowly expressed or not expressed at all (≤ 4 Transcripts Per Million [TPM]) in the uninfected condition (median TPM of 0.37 in Tnms42 and 0.46 in midgut cells). In the infected condition, a total of 62 and 187 TE families were differentially expressed (DE) in midgut and Tnms42 cells, respectively, with more up- (46) than down- (16) regulated TE families in the former and as many up- (91) as down- (96) regulated TEs in the latter. Expression log2 fold changes of DE TE families varied from -4.95 to 9.11 in Tnms42 cells, and from -4.28 to 7.66 in midgut. Large variations in expression profiles of DE TEs were observed depending on the type of cells and on time after infection. Overall, the impact of AcMNPV on TE expression in T. ni is moderate, but potentially sufficient to affect TE activity and genome architecture. Interestingly, one host-derived TE integrated into AcMNPV genomes is highly expressed in infected Tnms42 cells. This result shows that virus-borne TEs can be expressed, further suggesting that they may be able to transpose, and that viruses may act as vectors of horizontal transfer of TEs in insects.

Development and ultrastructure of sex-pheromone gland cells in females of the cabbage looper moth, Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae)

1979 ◽  
Vol 57 (1) ◽  
pp. 220-236 ◽  
Author(s):  
Jean Percy
Keyword(s):  
Sex Pheromone ◽  
Trichoplusia Ni ◽  
Cabbage Looper ◽  
Apical Plasma Membrane ◽  
Tubular Structures ◽  
Gland Cells ◽  
Cabbage Looper Moth ◽  
Sex Pheromone Gland ◽  
Lepidoptera Noctuidae ◽  
Tubular Endoplasmic Reticulum

The sex-pheromone-producing gland in female Trichoplusia ni (Hübner) is a modified intersegmental membrane and the gland cells are ductless. Lipid spheres are located throughout gland cells and vary both in number and size relative to the age of the female. Most of the lipid is surrounded by oval to elongate distensions of smooth tubular endoplasmic reticulum which contain the enzyme catalase and are thus microperoxisomes. Lipid spheres evert the apical plasma membrane between microvilli, move away from the gland cells, and are stored in the cuticle as discrete lipid deposits. These deposits, in turn, move to the surface of the gland by tubular structures that differ from epicuticular filaments.

Activity of perfluorobutyl-containing components in pheromone blend of cabbage looper moth,Trichoplusia ni

1992 ◽  
Vol 18 (5) ◽  
pp. 737-748 ◽  
Author(s):  
Charles Linn ◽  
Wendell Roelofs ◽  
Wei-Chuan Sun ◽  
Glenn D. Prestwich
Keyword(s):  
Trichoplusia Ni ◽  
Cabbage Looper ◽  
Cabbage Looper Moth ◽  
Pheromone Blend

Cloning and functional expression of a cDNA encoding a metabolic acyl-CoA Δ9-desaturase of the cabbage looper moth, Trichoplusia ni

1999 ◽  
Vol 29 (5) ◽  
pp. 435-443 ◽  
Author(s):  
Weitian Liu ◽  
Peter W.K. Ma ◽  
Patricia Marsella-Herrick ◽  
Claire-Lise Rosenfield ◽  
Douglas C. Knipple ◽  
...  
Keyword(s):  
Trichoplusia Ni ◽  
Functional Expression ◽  
Cabbage Looper ◽  
Cabbage Looper Moth ◽  
Δ9 Desaturase

The failure to discriminate: superparasitism ofTrichoplusia niHübner by a generalist tachinid parasitoid

2009 ◽  
Vol 100 (3) ◽  
pp. 255-261 ◽  
Author(s):  
V. Caron ◽  
J.H. Myers ◽  
D.R. Gillespie
Keyword(s):  
Sex Ratio ◽  
Host Selection ◽  
Trichoplusia Ni ◽  
Cabbage Looper ◽  
Pupal Weight ◽  
Host Quality ◽  
Host Discrimination ◽  
Host Interactions ◽  
Hymenopteran Parasitoids ◽  
The Impact

AbstractParasitoids are dependent on their hosts, which provide all of the resources for larval development. Parasitoid fitness, therefore, is directly related to the host quality, as determined by host size, age and health (e.g. parasitisation status); and this can only be assessed by the female parasitoid during host selection. Most studies of parasitoid-host interactions have focused on hymenopteran parasitoids rather than dipterans that are believed to be less discriminating during host selection. We assessed the impact of host quality and superparasitism on parasitoid fitness inCompsilura concinnataMeigen, a gregarious tachinid dipteran parasitoid, and its lepidopteran host the cabbage looper,Trichoplusia niHübner.FemaleC. concinnataparasitised all host stages ofT. nithat were presented to them, but emergence rates were higher from older hosts. Females readily superparasitised hosts. The number of flies emerging was higher from hosts parasitised at later instars, and flies emerged earlier from heavily superparasitised hosts. Superparasitism decreased parasitoid pupal weight and development time, indicating intra-host competition between parasitoid larvae and skewed the parasitoid sex ratio in favour of males.Host discrimination does not seem to be well developed inC. concinnata. Hosts are superparasitised despite the effects of superparasitism on offspring and sex ratio. This could be due to the wide host range ofC. concinnata; avoiding high superparasitism could occur naturally due to host switching and, therefore, developing host discrimination mechanisms for one host species may not be crucial.

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