scholarly journals OM MUTATIONS IN DROSOPHILA ANANASSAE ARE LINKED TO INSERTIONS OF A TRANSPOSABLE ELEMENT

Genetics ◽  
1986 ◽  
Vol 114 (1) ◽  
pp. 125-135
Author(s):  
Antony E Shrimpton ◽  
Elizabeth A Montgomery ◽  
Charles H Langley
Keyword(s):  
In Situ Hybridization ◽  
Genetic Mapping ◽  
Transposable Element ◽  
Southern Blotting ◽  
Spontaneous Mutation ◽  
Drosophila Ananassae ◽  
Homologous Region ◽  
Eye Morphology ◽  
Om Mutations

ABSTRACT It has been hypothesized that Om mutability in Drosophila ananassae (involving spontaneous mutation at 20 loci, resulting in semidominant, nonpleiotropic eye morphology defects) was due to insertion of a transposable element, tom. One particularly unstable Χ-linked Om allele produced several derivatives, one of which has a more extreme Om phenotype and was accompanied by a singed bristle mutant, sn9g. DNA probes from the sn locus of D. melanogaster were used to clone the homologous region of D. ananassae. Analysis of sn9g DNA detected a 6.5-kb insert. Genomic Southern blotting and in situ hybridization techniques showed that this insert is repetitive and dispersed. The existence of the tom element is supported by genetic mapping that established homology between the 6.5-kb sn9g insert and Om mutants at the four Χ-linked loci tested.

Assignment1 of maltase glucoamylase (MGAM) to pig chromosome 2 (2q21) by fluorescence in situ hybridization and confirmation by genetic mapping

2000 ◽  
Vol 90 (3-4) ◽  
pp. 236-237 ◽  
Author(s):  
J.H. Calvo ◽  
N.L. Lopez-Corrales ◽  
R. Osta ◽  
T.M. Skinner ◽  
S.I. Anderson ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genetic Mapping ◽  
Fluorescence In Situ Hybridization ◽  
Chromosome 2

Genetic mapping1 of the mouse homologue of the human angiopoietin-1 gene (Agpt) to mouse chromosome 9E2 by in situ hybridization

1999 ◽  
Vol 87 (3-4) ◽  
pp. 199-200
Author(s):  
N. Marziliano ◽  
S. Crovella ◽  
E. Audero ◽  
V. Pecile ◽  
F. Bussolino ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Genetic Mapping ◽  
Mouse Chromosome ◽  
Mouse Homologue ◽  
Angiopoietin 1

scholarly journals Fibrinogen is not synthesized by human megakaryocytes

Blood ◽  
1991 ◽  
Vol 77 (2) ◽  
pp. 311-316 ◽  
Author(s):  
F Louache ◽  
N Debili ◽  
E Cramer ◽  
J Breton-Gorius ◽  
W Vainchenker
Keyword(s):  
In Situ Hybridization ◽  
Southern Blotting ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Gene Transcript ◽  
Lambda Phage ◽  
Immunomagnetic Bead ◽  
Polymerase Chain ◽  
Negative Controls

Abstract The origin of platelet fibrinogen is controversial. It may arise from two sources: (a) exogenously by endocytosis of plasma fibrinogen, or (b) endogenously by synthesis. We explored the second possibility because we previously demonstrated that the first mechanism does occur. Fibrinogen synthesis by human megakaryocytes (MK) was investigated by in situ hybridization and the polymerase chain reaction (PCR) applied to mRNA. MK differentiating from marrow CFU-MK were cultured in suspension. In situ hybridization using the 35S alpha and beta fibrinogen chain anti-sense riboprobes was totally negative in MK in comparison with negative controls (lambda phage and alpha and beta fibrinogen chain sense riboprobes). In contrast, synthesis of fibrinogen was detected by this technique in a hepatoma cell line (HepG 2). Furthermore, mRNA for alpha and beta chains of fibrinogen was not detected by the PCR performed on mRNA from cultured MK enriched to 90% purity, by the immunomagnetic bead technique, even after Southern blotting of the amplified products. In addition, fibrinogen mRNA was undetected in marrow MK and in platelets by the same technique, whereas a specific megakaryocyte gene transcript (GPIb alpha) was easily detected. These observations demonstrate that the only mechanism responsible for the presence of fibrinogen in platelets is endocytosis of fibrinogen from plasma.

The Om(1E) Mutation in Drosophila ananassae Causes Compound Eye Overgrowth due to tom Retrotransposon-Driven Overexpression of a Novel Gene

Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1257-1270
Author(s):  
Naoto Juni ◽  
Takeshi Awasaki ◽  
Kiyohito Yoshida ◽  
Samuel H Hori
Keyword(s):  
Compound Eye ◽  
Transmembrane Domain ◽  
Drosophila Ananassae ◽  
Embryonic Cells ◽  
Chromosome Walking ◽  
The Central Nervous System ◽  
Insertion Sites ◽  
Om Mutations ◽  
Novel Protein

Abstract Optic morphology (Om) mutations in Drosophila ananassae are a group of retrotransposon (tom)-induced gain-of-function mutations that map to at least 22 independent loci and exclusively affect the compound eye morphology. In marked contrast to other Om mutations, which are characterized by fewer-than-normal and disorganized ommatidia, the Om(1E) mutation exhibits a peculiar phenotype as enlarged eyes with regularly arrayed normal ommatidia. To characterize the Om(1E) mutation, we have carried out molecular analyses. A putative Om(1E) locus cloned by tom tagging and chromosome walking contained two transcribed regions in the vicinity of tom insertion sites of the Om(1E) mutant alleles, and one of these regions was shown to be the Om(1E) gene by Pelement-mediated transformation experiments with D. melanogaster. The Om(1E) gene encodes a novel protein having potential transmembrane domain(s). In situ hybridization analyses demonstrated that the Om(1E) gene is expressed ubiquitously in embryonic cells, imaginal discs, and the cortex of the central nervous system of third instar larvae, and specifically in lamina precursor cells. Artificially induced ubiquitous overexpression of Om(1E) affected morphogenesis of wing imaginal disc derivatives or large bristle formation. These findings suggest that the Om(1E) gene is involved in a variety of developmental processes.

Genetic Mapping of the Adh Locus in the Repleta Group of Drosophila by in situ Hybridization

1990 ◽  
Vol 81 (1) ◽  
pp. 83-86 ◽  
Author(s):  
M. Labrador ◽  
H. Naveira ◽  
A. Fontdevila
Keyword(s):  
In Situ Hybridization ◽  
Genetic Mapping ◽  
Repleta Group

Refined physical mapping of the Sec-1 locus on the satellite of chromosome 1R of rye (Secale cereale)

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 889-893 ◽  
Author(s):  
W. Busch ◽  
R. G. Herrmann ◽  
R. Martin
Keyword(s):  
In Situ Hybridization ◽  
Genetic Mapping ◽  
Fluorescence In Situ Hybridization ◽  
Physical Mapping ◽  
Secale Cereale ◽  
Secondary Constriction ◽  
Heterologous Hybridization ◽  
Secale Cereale L ◽  
Chromosome 1R

The Sec-1 locus (ω-secalin) of rye (Secale cereale L.) was mapped in the satellite of the short arm of chromosome 1R using fluorescence in situ hybridization and a genomic probe called pSec2B. Sec-1 is located in the middle of the satellite at the junction of the proximal euchromatic and the distal heterochromatic regions. Double hybridization experiments using rDNA and pSec2B showed that the NOR spans over the secondary constriction of the short arm of chromosome 1R and that there is a clearly visible gap between the NOR and Sec-1. Heterologous hybridization of pSec2B to barley visualized the B-hordein locus on chromosome 1H.Key words: fluorescence in situ hybridization, physical mapping, genetic mapping, secalin, rye, B-hordein, rDNA.

Assignment1 of SYNJ1 to human chromosome 21q22.2 and Synj12 to the murine homologous region on chromosome 16C3–4 by in situ hybridization

2000 ◽  
Vol 88 (1-2) ◽  
pp. 89-90 ◽  
Author(s):  
O. Cremona ◽  
M. Nimmakayalu ◽  
C. Haffner ◽  
P. Bray-Ward ◽  
D.C. Ward ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Chromosome ◽  
Homologous Region
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