scholarly journals Co-localization to chromosome bands 99E1-3 of the Drosophila melanogaster myosin light chain-2 gene and a haplo-insufficient locus that affects flight behavior.

Genetics ◽  
1989 ◽  
Vol 122 (1) ◽  
pp. 139-151 ◽  
Author(s):  
J W Warmke ◽  
A J Kreuz ◽  
S Falkenthal
Keyword(s):  
Drosophila Melanogaster ◽  
Light Chain ◽  
Mutant Allele ◽  
Myosin Light Chain ◽  
Complementation Group ◽  
Flight Behavior ◽  
Myosin Light Chain 2 ◽  
Complementation Groups ◽  
Single Allele ◽  
Chromosome Bands

Abstract Using overlapping synthetic deficiencies, we find that a haplo-insufficient locus affecting flight behavior and the myosin light chain-2 gene co-map to the Drosophila melanogaster polytene chromosome interval 99D9-E1 to 99E2-3. From screening over 9000 EMS-treated chromosomes, we obtained alleles of two complementation groups that map to this same interval. One of these complementation groups lfm(3)99Eb, exhibits dominant flightless behavior; thus, flightless behavior of the deficiency is in all likelihood due to hemizygosity of this single locus. Rescue of flightless behavior by a duplication indicates that the single allele, E38, of the Ifm(3)99Eb complementation group is a hypomorph. Based upon its map position and a reduction in concentration of myosin light chain-2 mRNA in heterozygotes, we propose that Ifm(3)Eb(E38) is a mutant allele of the myosin light chain-2 gene. Our genetic analysis also resulted in the identification of four dominant flightless alleles of an unlinked locus, l(3)nc99Eb, that exhibits dominant lethal synergism with Ifm(3)99Eb.

scholarly journals Characterization of the myosin light-chain-2 gene of Drosophila melanogaster

1985 ◽  
Vol 5 (11) ◽  
pp. 3058-3068
Author(s):  
V P Parker ◽  
S Falkenthal ◽  
N Davidson
Keyword(s):  
Drosophila Melanogaster ◽  
Light Chain ◽  
Calcium Binding ◽  
Myosin Light Chain ◽  
Cdna Sequence ◽  
Recombinant Dna ◽  
In Vitro Translation ◽  
Cdna Clones ◽  
Chromosomal Locus ◽  
Myosin Light Chain 2

Recombinant DNA clones encoding the Drosophila melanogaster homolog of the vertebrate myosin light-chain-2 (MLC-2) gene have been isolated. This single-copy gene maps to the chromosomal locus 99E. The nucleotide sequence was determined for a 3.4-kilobase genomic fragment containing the gene and for two MLC-2 cDNA clones generated from late pupal mRNA. Comparison of these sequences shows that the gene contains two introns, the positions of which are conserved in the corresponding rat sequence. Extension of a primer homologous to the mRNA reveals two start sites for transcription 12 nucleotides apart. The sequence TATA is not present ahead of the mRNA cap site. There are two major sites of poly(A) addition separated by 356 nucleotides. The protein sequence derived from translation of the cDNA sequence shows a high degree of homology with that for the DTNB myosin light chain (MLC-2) of chicken. A lower degree of sequence homology was seen in comparisons with other evolutionarily related calcium-binding proteins. RNA blots show high levels of expression of several transcripts during the developmental time stages when muscle is being produced. In vitro translation of hybrid-selected RNA produces two polypeptides which comigrate on two-dimensional gels with proteins from Drosophila actomyosin, although the cDNA sequence reveals only one 26-kilodalton primary translation product.

scholarly journals LETHAL MUTATIONS FLANKING THE 68C GLUE GENE CLUSTER ON CHROMOSOME 3 OF DROSOPHILA MELANOGASTER

Genetics ◽  
1986 ◽  
Vol 112 (4) ◽  
pp. 785-802
Author(s):  
Madeline A Crosby ◽  
Elliot M Meyerowitz
Keyword(s):  
Drosophila Melanogaster ◽  
Gene Cluster ◽  
Xanthine Dehydrogenase ◽  
Complementation Group ◽  
Chromosome 3 ◽  
Relative Order ◽  
Lethal Mutations ◽  
Complementation Groups ◽  
Chromosome Bands ◽  
New Alleles

ABSTRACT We have conducted a genetic analysis of the region flanking the 68C glue gene cluster in Drosophila melanogaster by isolating lethal and semilethal mutations uncovered by deficiencies which span this region. Three different mutagens were used: ethyl methanesulfonate (EMS), ethyl nitrosourea (ENU) and diepoxybutane (DEB). In the region from 68A3 to 68C11, 64 lethal, semilethal, and visible mutations were recovered. These include alleles of 13 new lethal complementation groups, as well as new alleles of rotated, low xanthine dehydrogenase, lethal(3)517 and lethal(3)B76. Six new visible mutations from within this region were recovered on the basis of their reduced viability; all proved to be semiviable alleles of lethal complementation groups. No significant differences were observed in the distributions of lethals recovered using the three different mutagens. Each lethal was mapped on the basis of complementation with overlapping deficiencies; mutations that mapped within the same interval were tested for complementation, and the relative order of the lethal groups within each interval was determined by recombination. The cytological distribution of genes within the 68A3-68C11 region is not uniform: the region from 68A2,3 to 68B1,3 (seven to ten polytene chromosome bands) contains at least 13 lethal complementation groups and the mutation low xanthine dehydrogenase; the adjoining region from 68B1,3 to 68C5,6 (six to nine bands) includes the 68C glue gene cluster, but no known lethal or visible complementation groups; and the interval from 68C5,6 to 68C10,11 (three to five bands) contains at least three lethal complementation groups and the visible mutation rotated. The developmental stage at which lethality is observed was determined for a representative allele from each lethal complementation group.

scholarly journals Characterization of the myosin light-chain-2 gene of Drosophila melanogaster.

1985 ◽  
Vol 5 (11) ◽  
pp. 3058-3068 ◽  
Author(s):  
V P Parker ◽  
S Falkenthal ◽  
N Davidson
Keyword(s):  
Drosophila Melanogaster ◽  
Light Chain ◽  
Calcium Binding ◽  
Myosin Light Chain ◽  
Cdna Sequence ◽  
Recombinant Dna ◽  
In Vitro Translation ◽  
Cdna Clones ◽  
Chromosomal Locus ◽  
Myosin Light Chain 2

Recombinant DNA clones encoding the Drosophila melanogaster homolog of the vertebrate myosin light-chain-2 (MLC-2) gene have been isolated. This single-copy gene maps to the chromosomal locus 99E. The nucleotide sequence was determined for a 3.4-kilobase genomic fragment containing the gene and for two MLC-2 cDNA clones generated from late pupal mRNA. Comparison of these sequences shows that the gene contains two introns, the positions of which are conserved in the corresponding rat sequence. Extension of a primer homologous to the mRNA reveals two start sites for transcription 12 nucleotides apart. The sequence TATA is not present ahead of the mRNA cap site. There are two major sites of poly(A) addition separated by 356 nucleotides. The protein sequence derived from translation of the cDNA sequence shows a high degree of homology with that for the DTNB myosin light chain (MLC-2) of chicken. A lower degree of sequence homology was seen in comparisons with other evolutionarily related calcium-binding proteins. RNA blots show high levels of expression of several transcripts during the developmental time stages when muscle is being produced. In vitro translation of hybrid-selected RNA produces two polypeptides which comigrate on two-dimensional gels with proteins from Drosophila actomyosin, although the cDNA sequence reveals only one 26-kilodalton primary translation product.

scholarly journals Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis

1994 ◽  
Vol 269 (24) ◽  
pp. 16961-16970
Author(s):  
S.W. Kubalak ◽  
W.C. Miller-Hance ◽  
T.X. O'Brien ◽  
E. Dyson ◽  
K.R. Chien
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Myosin Light Chain 2 ◽  
Atrial Myosin
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