DNA Sequence Analysis of Spontaneous Mutagenesis in Saccharomyces cerevisiae

Genetics ◽  
1998 ◽  
Vol 148 (4) ◽  
pp. 1491-1505 ◽  
Author(s):  
Bernard A Kunz ◽  
Karthikeyan Ramachandran ◽  
Edward J Vonarx
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Budding Yeast ◽  
Spontaneous Mutation ◽  
Dna Sequence Analysis ◽  
Mutation Rates ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

AbstractTo help elucidate the mechanisms involved in spontaneous mutagenesis, DNA sequencing has been applied to characterize the types of mutation whose rates are increased or decreased in mutator or antimutator strains, respectively. Increased spontaneous mutation rates point to malfunctions in genes that normally act to reduce spontaneous mutation, whereas decreased rates are associated with defects in genes whose products are necessary for spontaneous mutagenesis. In this article, we survey and discuss the mutational specificities conferred by mutator and antimutator genes in the budding yeast Saccharomyces cerevisiae. The implications of selected aspects of the data are considered with respect to the mechanisms of spontaneous mutagenesis.

DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae

1988 ◽  
Vol 8 (2) ◽  
pp. 978-981
Author(s):  
C N Giroux ◽  
J R Mis ◽  
M K Pierce ◽  
S E Kohalmi ◽  
B A Kunz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Transposable Elements ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Base Pair ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

A collection of 196 spontaneous mutations in the SUP4-o gene of the yeast Saccharomyces cerevisiae was analyzed by DNA sequencing. The classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. Our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in S. cerevisiae.

scholarly journals DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae.

1988 ◽  
Vol 8 (2) ◽  
pp. 978-981 ◽  
Author(s):  
C N Giroux ◽  
J R Mis ◽  
M K Pierce ◽  
S E Kohalmi ◽  
B A Kunz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Transposable Elements ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Base Pair ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

A collection of 196 spontaneous mutations in the SUP4-o gene of the yeast Saccharomyces cerevisiae was analyzed by DNA sequencing. The classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. Our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in S. cerevisiae.

Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae

1987 ◽  
Vol 7 (3) ◽  
pp. 1198-1207 ◽  
Author(s):  
R Rothstein ◽  
C Helms ◽  
N Rosenberg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Gene Conversion ◽  
Dna Sequence ◽  
Suppressor Gene ◽  
Mitotic Recombination ◽  
Dna Sequence Analysis ◽  
Repeated Sequences ◽  
Repair Gene

Deletions of a tyrosine tRNA suppressor gene, SUP4-o, are mediated by recombination between short repeated delta sequences in Saccharomyces cerevisiae. The arrangement of the five solo delta sequences that surround the SUP4 locus was established by DNA sequence analysis. Seven deletion classes were identified by genomic blotting. DNA sequence analysis also showed that the delta sequences within a 6.5-kilobase region of the SUP4 locus were the endpoints of these events. In three of these classes, an adjacent interval surrounded by delta sequences was inverted in concert with the deletion. The frequency of all deletion classes decreased in strains that contained mutations in the recombination and repair gene RAD52. We present two gene conversion mechanisms by which these rearrangements could have been generated. These models may also explain deletions between repeated sequences in other systems.

Spontaneous mutagenesis in Escherichia coli harbouring plasmid pKM101: DNA sequence analysis of forward lacI− mutations

Mutagenesis ◽  
1993 ◽  
Vol 8 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Alasdair J.E. Gordon ◽  
Cecilia Bernelot-Moens ◽  
Barry W. Glickman
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutagenesis ◽  
Plasmid Pkm101

scholarly journals Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae.

1987 ◽  
Vol 7 (3) ◽  
pp. 1198-1207 ◽  
Author(s):  
R Rothstein ◽  
C Helms ◽  
N Rosenberg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Gene Conversion ◽  
Dna Sequence ◽  
Suppressor Gene ◽  
Mitotic Recombination ◽  
Dna Sequence Analysis ◽  
Repeated Sequences ◽  
Repair Gene

Deletions of a tyrosine tRNA suppressor gene, SUP4-o, are mediated by recombination between short repeated delta sequences in Saccharomyces cerevisiae. The arrangement of the five solo delta sequences that surround the SUP4 locus was established by DNA sequence analysis. Seven deletion classes were identified by genomic blotting. DNA sequence analysis also showed that the delta sequences within a 6.5-kilobase region of the SUP4 locus were the endpoints of these events. In three of these classes, an adjacent interval surrounded by delta sequences was inverted in concert with the deletion. The frequency of all deletion classes decreased in strains that contained mutations in the recombination and repair gene RAD52. We present two gene conversion mechanisms by which these rearrangements could have been generated. These models may also explain deletions between repeated sequences in other systems.

scholarly journals Modified protocol for DNA sequence analysis using the Sequenase™ version 2.0 DNA Sequencing Kit

2001 ◽  
Vol 6 (1) ◽  
pp. 46-47
Author(s):  
Mickey M. Martin ◽  
Xylophone Victor ◽  
Xin Zhao ◽  
Terry S. Elton
Keyword(s):  
Sequence Analysis ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Version 2.0
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