Spontaneous mutagenesis in Escherichia coli harbouring plasmid pKM101: DNA sequence analysis of forward lacI− mutations

Mutagenesis ◽  
1993 ◽  
Vol 8 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Alasdair J.E. Gordon ◽  
Cecilia Bernelot-Moens ◽  
Barry W. Glickman
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutagenesis ◽  
Plasmid Pkm101

DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae

1988 ◽  
Vol 8 (2) ◽  
pp. 978-981
Author(s):  
C N Giroux ◽  
J R Mis ◽  
M K Pierce ◽  
S E Kohalmi ◽  
B A Kunz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Transposable Elements ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Base Pair ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

A collection of 196 spontaneous mutations in the SUP4-o gene of the yeast Saccharomyces cerevisiae was analyzed by DNA sequencing. The classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. Our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in S. cerevisiae.

DNA Sequence Analysis of Spontaneous Mutagenesis in Saccharomyces cerevisiae

Genetics ◽  
1998 ◽  
Vol 148 (4) ◽  
pp. 1491-1505 ◽  
Author(s):  
Bernard A Kunz ◽  
Karthikeyan Ramachandran ◽  
Edward J Vonarx
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Budding Yeast ◽  
Spontaneous Mutation ◽  
Dna Sequence Analysis ◽  
Mutation Rates ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

AbstractTo help elucidate the mechanisms involved in spontaneous mutagenesis, DNA sequencing has been applied to characterize the types of mutation whose rates are increased or decreased in mutator or antimutator strains, respectively. Increased spontaneous mutation rates point to malfunctions in genes that normally act to reduce spontaneous mutation, whereas decreased rates are associated with defects in genes whose products are necessary for spontaneous mutagenesis. In this article, we survey and discuss the mutational specificities conferred by mutator and antimutator genes in the budding yeast Saccharomyces cerevisiae. The implications of selected aspects of the data are considered with respect to the mechanisms of spontaneous mutagenesis.

Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli

1985 ◽  
Vol 186 (2) ◽  
pp. 283-293 ◽  
Author(s):  
Catherine M. Joyce ◽  
David M. Fujii ◽  
Helaina S. Laks ◽  
Cynthia M. Hughes ◽  
Nigel D.F. Grindley
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Genetic Mapping ◽  
Dna Sequence ◽  
Dna Sequence Analysis

scholarly journals Native Plasmids of Fusobacterium nucleatum: Characterization and Use in Development of Genetic Systems

2000 ◽  
Vol 182 (4) ◽  
pp. 1176-1180 ◽  
Author(s):  
Susan Kinder Haake ◽  
Sean C. Yoder ◽  
Gwynne Attarian ◽  
Kara Podkaminer
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Dna Sequence ◽  
Fusobacterium Nucleatum ◽  
Dna Sequence Analysis ◽  
Modification System ◽  
Restriction Modification System ◽  
Shuttle Plasmid ◽  
Genetic Systems ◽  
Restriction Modification

ABSTRACT Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.

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