DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae

1988 ◽  
Vol 8 (2) ◽  
pp. 978-981
Author(s):  
C N Giroux ◽  
J R Mis ◽  
M K Pierce ◽  
S E Kohalmi ◽  
B A Kunz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Transposable Elements ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Base Pair ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

A collection of 196 spontaneous mutations in the SUP4-o gene of the yeast Saccharomyces cerevisiae was analyzed by DNA sequencing. The classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. Our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in S. cerevisiae.

scholarly journals DNA sequence analysis of spontaneous mutations in the SUP4-o gene of Saccharomyces cerevisiae.

1988 ◽  
Vol 8 (2) ◽  
pp. 978-981 ◽  
Author(s):  
C N Giroux ◽  
J R Mis ◽  
M K Pierce ◽  
S E Kohalmi ◽  
B A Kunz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Transposable Elements ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Base Pair ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

A collection of 196 spontaneous mutations in the SUP4-o gene of the yeast Saccharomyces cerevisiae was analyzed by DNA sequencing. The classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. Our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in S. cerevisiae.

DNA Sequence Analysis of Spontaneous Mutagenesis in Saccharomyces cerevisiae

Genetics ◽  
1998 ◽  
Vol 148 (4) ◽  
pp. 1491-1505 ◽  
Author(s):  
Bernard A Kunz ◽  
Karthikeyan Ramachandran ◽  
Edward J Vonarx
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Dna Sequencing ◽  
Dna Sequence ◽  
Budding Yeast ◽  
Spontaneous Mutation ◽  
Dna Sequence Analysis ◽  
Mutation Rates ◽  
Yeast Saccharomyces Cerevisiae ◽  
Spontaneous Mutagenesis

AbstractTo help elucidate the mechanisms involved in spontaneous mutagenesis, DNA sequencing has been applied to characterize the types of mutation whose rates are increased or decreased in mutator or antimutator strains, respectively. Increased spontaneous mutation rates point to malfunctions in genes that normally act to reduce spontaneous mutation, whereas decreased rates are associated with defects in genes whose products are necessary for spontaneous mutagenesis. In this article, we survey and discuss the mutational specificities conferred by mutator and antimutator genes in the budding yeast Saccharomyces cerevisiae. The implications of selected aspects of the data are considered with respect to the mechanisms of spontaneous mutagenesis.

scholarly journals A tester system for detecting each of the six base-pair substitutions in Saccharomyces cerevisiae by selecting for an essential cysteine in iso-1-cytochrome c.

Genetics ◽  
1991 ◽  
Vol 128 (1) ◽  
pp. 59-67
Author(s):  
M Hampsey
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Cytochrome C ◽  
Dna Sequence ◽  
Base Pair ◽  
Carbon Sources ◽  
Methyl Methanesulfonate ◽  
Low Frequencies ◽  
Yeast Strains ◽  
Selection Medium

Abstract A collection of isogenic yeast strains that is specifically diagnostic for the six possible base-pair substitutions is described. Each strain contains a single, unique base-pair substitution at the Cys-22 codon of the CYC1 gene, which codes for iso-1-cytochrome c. These mutations encode replacements of the functionally critical Cys-22 and render each strain unable to grow on media containing nonfermentable carbon sources (Cyc-). Specific base-pair substitutions, which restore the Cys-22 codon, can be monitored simply by scoring for reversion to the Cyc+ phenotype. These strains revert spontaneously at very low frequencies and exhibit specific patterns of reversion in response to different mutagens. Only true (CYC1+) revertants were recovered after 7 days on selection medium. The following mutagen specificities were observed: ethyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine, G.C----A.T; 4-nitroquinoline-1-oxide, G.C----T.A and G.C----A.T; diepoxybutane, A.T----T.A, A.T----G.C and G.C----T.A; 5-azacytidine, G.C----C.G. Methyl methanesulfonate induced all six mutations, albeit at relatively low frequencies, with preference for A.T----T.A and A.T----G.C. Ultraviolet light was the most inefficient mutagen used in this study, consistent with its preference for transition mutations at dipyrimidine sequences reported in other systems. This tester system is valuable as a simple and reliable assay for specific mutations without DNA sequence analysis.

Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae

1987 ◽  
Vol 7 (3) ◽  
pp. 1198-1207 ◽  
Author(s):  
R Rothstein ◽  
C Helms ◽  
N Rosenberg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Gene Conversion ◽  
Dna Sequence ◽  
Suppressor Gene ◽  
Mitotic Recombination ◽  
Dna Sequence Analysis ◽  
Repeated Sequences ◽  
Repair Gene

Deletions of a tyrosine tRNA suppressor gene, SUP4-o, are mediated by recombination between short repeated delta sequences in Saccharomyces cerevisiae. The arrangement of the five solo delta sequences that surround the SUP4 locus was established by DNA sequence analysis. Seven deletion classes were identified by genomic blotting. DNA sequence analysis also showed that the delta sequences within a 6.5-kilobase region of the SUP4 locus were the endpoints of these events. In three of these classes, an adjacent interval surrounded by delta sequences was inverted in concert with the deletion. The frequency of all deletion classes decreased in strains that contained mutations in the recombination and repair gene RAD52. We present two gene conversion mechanisms by which these rearrangements could have been generated. These models may also explain deletions between repeated sequences in other systems.

DNA sequence analysis of spontaneous mutations at a LacZ transgene integrated on the mouse X chromosome

Mutagenesis ◽  
1993 ◽  
Vol 8 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Jan A. Gossen ◽  
Wiljo J.F. de Leeuw ◽  
Arjen Q. Bakker ◽  
Jan Vijg
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
X Chromosome ◽  
Dna Sequence Analysis ◽  
Spontaneous Mutations

scholarly journals Concerted deletions and inversions are caused by mitotic recombination between delta sequences in Saccharomyces cerevisiae.

1987 ◽  
Vol 7 (3) ◽  
pp. 1198-1207 ◽  
Author(s):  
R Rothstein ◽  
C Helms ◽  
N Rosenberg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Gene Conversion ◽  
Dna Sequence ◽  
Suppressor Gene ◽  
Mitotic Recombination ◽  
Dna Sequence Analysis ◽  
Repeated Sequences ◽  
Repair Gene

Deletions of a tyrosine tRNA suppressor gene, SUP4-o, are mediated by recombination between short repeated delta sequences in Saccharomyces cerevisiae. The arrangement of the five solo delta sequences that surround the SUP4 locus was established by DNA sequence analysis. Seven deletion classes were identified by genomic blotting. DNA sequence analysis also showed that the delta sequences within a 6.5-kilobase region of the SUP4 locus were the endpoints of these events. In three of these classes, an adjacent interval surrounded by delta sequences was inverted in concert with the deletion. The frequency of all deletion classes decreased in strains that contained mutations in the recombination and repair gene RAD52. We present two gene conversion mechanisms by which these rearrangements could have been generated. These models may also explain deletions between repeated sequences in other systems.

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