scholarly journals CONCERNING THE MECHANISM OF ULTRAVIOLET MUTAGENESIS. A MICROMANIPULATORY PEDIGREE ANALYSIS IN SCHIZOSACCHAROMYCES POMBE

Genetics ◽  
1967 ◽  
Vol 57 (1) ◽  
pp. 169-178
Author(s):  
K Haefner
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Pedigree Analysis ◽  
Ultraviolet Mutagenesis

scholarly journals Analysis of the genetic instability induced by nitrous acid in Schizosaccharomyces pombe

1968 ◽  
Vol 12 (1) ◽  
pp. 45-54 ◽  
Author(s):  
N. Loprieno ◽  
A. Abbondandolo ◽  
S. Bonatti ◽  
R. Guglielminetti
Keyword(s):  
Genetic Analysis ◽  
Schizosaccharomyces Pombe ◽  
Genetic Instability ◽  
Nitrous Acid ◽  
Pedigree Analysis ◽  
Genetic Alteration ◽  
Mutagenic Treatment ◽  
Interallelic Complementation ◽  
Cell Colony

A pedigree analysis of several cell-colony generations following a mutagenic treatment with nitrous acid has shown that in S. pombe a genetic instability is produced that replicates several times and produces a mutation in independent lines.It has been shown that the mutants isolated in the progeny of a mosaic colony all contain a genetic alteration that cannot be resolved by genetic analysis and therefore the mutations have occurred at the same genetic site. This finding is confirmed by interallelic complementation and phenotypic analyses.

Mycelial and syncytial growth in Schizosaccharomyces pombe induced by novel septation mutations

1993 ◽  
Vol 104 (2) ◽  
pp. 485-493 ◽  
Author(s):  
M. Sipiczki ◽  
B. Grallert ◽  
I. Miklos
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Pedigree Analysis ◽  
Restrictive Temperature ◽  
Triple Mutant ◽  
Microtubule Inhibitor ◽  
Beta Glucanase ◽  
Septum Formation ◽  
Increased Sensitivity ◽  
The Right ◽  
Mutant Forms

Mutation in the gene sep1+ of the unicellular fission yeast Schizosaccharomyces pombe impairs cell separation after cytokinesis and confers a branching mycelial morphology. The mutant is not defective in cell wall beta-glucanase activity but shows increased sensitivity to Ca2+ and Mg2+, and increased resistance to the microtubule inhibitor benomyl. The mycelial growth of sep1-1 provides a convenient method for the examination of the polar growth pattern and for pedigree analysis as demonstrated by the segregation of mating types in the homothallic microhyphae. sep1 is closely linked to ade1 (0.94 cM) on the right arm of chromosome II. The ts mutation spl1-1 confers a bent cell shape and causes aberrant septum formation at the restrictive temperature. sep1+ and spl1+ perform closely related functions as their mutant alleles interact with each other and with another septation mutant cdc4-8. These functions may overlap with certain cytoskeletal processes and with the determination of cell polarity because the triple mutant forms huge multinucleate syncytia with promiscuous branching and rare septum formation.

Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element

1986 ◽  
Vol 6 (1) ◽  
pp. 80-89 ◽  
Author(s):  
W D Heyer ◽  
M Sipiczki ◽  
J Kohli
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Pedigree Analysis ◽  
Sequence Specificity ◽  
Low Frequencies ◽  
Shuttle Vectors ◽  
Copy Numbers ◽  
Low Copy Number ◽  
Meiotic Stability ◽  
Dna Fragment ◽  
Mitosis And Meiosis

We characterized a number of widely used yeast-Escherichia coli shuttle vectors in the fission yeast Schizosaccharomyces pombe. The 2 micron vectors pDB248 and YEp13 showed high frequency of transformation, intermediate mitotic and low meiotic stability, and a low copy number in S. pombe, analogous to their behavior in [cir0] strains of Saccharomyces cerevisiae. The S. cerevisiae integration vectors pLEU2 and pURA3 transformed S. pombe at very low frequencies but, surprisingly, in a nonintegrative fashion. Instead, they replicated autonomously, and they showed very high copy numbers (up to 150 copies per plasmid-containing cell). This could reflect a lack of sequence specificity for replication of plasmid DNA in S. pombe. pFL20, an S. pombe ars vector, and a series of plasmids derived from it were studied to analyze the unusually high stability of this plasmid. Mitotic stability and partitioning of the plasmids was measured by pedigree analysis of transformed S. pombe cells. An S. pombe DNA fragment (stb) was identified that stabilizes pFL20 by improvement of plasmid partitioning in mitosis and meiosis.

scholarly journals Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element.

1986 ◽  
Vol 6 (1) ◽  
pp. 80-89 ◽  
Author(s):  
W D Heyer ◽  
M Sipiczki ◽  
J Kohli
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Pedigree Analysis ◽  
Sequence Specificity ◽  
Low Frequencies ◽  
Shuttle Vectors ◽  
Copy Numbers ◽  
Low Copy Number ◽  
Meiotic Stability ◽  
Dna Fragment ◽  
Mitosis And Meiosis

We characterized a number of widely used yeast-Escherichia coli shuttle vectors in the fission yeast Schizosaccharomyces pombe. The 2 micron vectors pDB248 and YEp13 showed high frequency of transformation, intermediate mitotic and low meiotic stability, and a low copy number in S. pombe, analogous to their behavior in [cir0] strains of Saccharomyces cerevisiae. The S. cerevisiae integration vectors pLEU2 and pURA3 transformed S. pombe at very low frequencies but, surprisingly, in a nonintegrative fashion. Instead, they replicated autonomously, and they showed very high copy numbers (up to 150 copies per plasmid-containing cell). This could reflect a lack of sequence specificity for replication of plasmid DNA in S. pombe. pFL20, an S. pombe ars vector, and a series of plasmids derived from it were studied to analyze the unusually high stability of this plasmid. Mitotic stability and partitioning of the plasmids was measured by pedigree analysis of transformed S. pombe cells. An S. pombe DNA fragment (stb) was identified that stabilizes pFL20 by improvement of plasmid partitioning in mitosis and meiosis.

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