Non-budding Hydra: Form Regulation and Bud Induction

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

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Ethylene and carbohydrate requirements for apogamous bud induction in Pteridium gametophytes

Canadian Journal of Botany ◽

10.1139/b74-268 ◽

1974 ◽

Vol 52 (9) ◽

pp. 2089-2096 ◽

Cited By ~ 3

Author(s):

H. W. Elmore ◽

D. P. Whittier

Keyword(s):

Carbon Dioxide ◽

Competitive Inhibitor ◽

Bud Induction ◽

Endogenous Ethylene ◽

Exogenous Ethylene

Periodic evacuations of culture vessels containing Pteridium gametophytes to reduce the concentration of endogenous ethylene within the tissue, followed by reinfiltration with air, yielded a 99% reduction in apogamous budding as compared to the ethylene-reinfiltrated control. Growing gametophytes in increasing concentrations of carbon dioxide, a competitive inhibitor of ethylene, caused decreases in the apogamous response. Apogamy was completely eliminated by 7% carbon dioxide, without inhibiting gametophytic growth. Adding 1 μl/liter ethylene to each concentration of carbon dioxide reversed the inhibition and some apogamy did occur in gametophytes grown in 7% carbon dioxide supplied concomitantly with the exogenous ethylene. A carbohydrate is required for ethylene-induced apogamous bud induction. Growth of gametophytes in the presence of ethylene on medium lacking sucrose for extended experimental periods was not sufficient to cause induction. Total gametophytic growth on media containing various concentrations of sucrose was not affected by the presence or absence of ethylene in the atmosphere surrounding colonies. These results demonstrate that both ethylene and a utilizable carbohydrate are required for apogamy.

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IS AUXIN EXPORT OF APPLE FRUIT AN ALTERNATIVE SIGNAL FOR INHIBITION OF FLOWER BUD INDUCTION ?

Acta Horticulturae ◽

10.17660/actahortic.1998.463.33 ◽

1998 ◽

pp. 271-278 ◽

Cited By ~ 3

Author(s):

R. Callejas ◽

F. Bangerth

Keyword(s):

Apple Fruit ◽

Flower Bud ◽

Bud Induction

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Disease-resistant transgenic adzuki bean plants obtained through an efficient transformation system

Crop and Pasture Science ◽

10.1071/cp12300 ◽

2012 ◽

Vol 63 (12) ◽

pp. 1090 ◽

Cited By ~ 1

Author(s):

Huatao Chen ◽

Xin Chen ◽

Heping Gu ◽

Xingxing Yuan ◽

Hongmei Zhang ◽

...

Keyword(s):

Soybean Mosaic Virus ◽

Shoot Elongation ◽

Zeatin Riboside ◽

Medium Composition ◽

Adventitious Bud ◽

Adzuki Bean ◽

Transformation System ◽

Bean Plants ◽

Bud Induction

An efficient regeneration and transformation system was established and optimised for adzuki bean (Vigna angularis (Willd.) Ohwi & Ohashi). 6-Benzylaminopurine at 5 mg L–1 was used to increase adventitious bud induction frequency. The highest frequency of shoot elongation was 92.8% when using a medium composition of MS salts combined with 0.1 mg L–1 of IAA, 0.5 mg L–1 of GA3, 1.0 mg L–1 of zeatin-riboside, 50 mg L–1 of aspartic acid, and 50 mg L–1 of glutamic acid. In vitro rooting was 100% when shoots were cultured on the solid MS medium supplemented with 1.0 mg L–1 of NAA. Reproducible transformation of epicotyl explants was developed using the A. tumefaciens EHA105 strain. Using a concentration of 40 mg L–1 of acetosyringone, 20 mm MES, and 5 mg L–1 of 6-benzylaminopurine in the co-cultivation medium, a transformation efficiency of 12.6% was attained. Using this transformation protocol, we obtained transgenic adzuki bean plants resistant to soybean mosaic virus by introducing the V. angularis VaPR3 gene.

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