scholarly journals Flowcytometric and ImageStream RNA-FISH gene expression, quantification and phenotypic characterization of blood and liver stages from human malaria species

2021 ◽  
Author(s):  
Camilla Luiza-Batista ◽  
Flore Nardella ◽  
Sabine Thiberge ◽  
Malika Serra-Hassoun ◽  
Marcelo U Ferreira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Phenotypic Characterization ◽  
Liver Stage ◽  
Rna Fish ◽  
Expression Studies ◽  
Fundamental Research ◽  
Gene Expression Quantification ◽  
Malaria Species ◽  
Gene Expression Studies

Abstract We adapted the RNA FISH stellaris® method to specifically detect the expression of Plasmodium genes by flowcytometry and ImageStream (FlowFISH). This new method accurately quantified the erythrocytic forms of P. falciparum and vivax, and the sexual stages of P. vivax from patient isolates. In addition, ImageStream analysis of liver stage sporozoites using a combination of surface CSP (Circumsporozoite Protein), DNA and 18S RNA labelling proved that the new FlowFISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.

Molecular Characterization of Mn-superoxide Dismutase and Gene Expression Studies in Dietary Restricted Brachionus plicatilis Rotifers

Hydrobiologia ◽  
2005 ◽  
Vol 546 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Gen Kaneko ◽  
Tatsuki Yoshinaga ◽  
Yoshiko Yanagawa ◽  
Shigeharu Kinoshita ◽  
Katsumi Tsukamoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Superoxide Dismutase ◽  
Molecular Characterization ◽  
Brachionus Plicatilis ◽  
Expression Studies ◽  
Gene Expression Studies

Establishment and characterization of a piscean PCF cell line for toxicity and gene expression studies as in vitro model

2014 ◽  
Vol 46 (3) ◽  
pp. 206-212 ◽  
Author(s):  
M. Goswami ◽  
B.S. Sharma ◽  
Kamalendra Yadav ◽  
S.N. Bahuguna ◽  
W.S. Lakra
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
In Vitro Model ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Vitro Model

Establishment and Characterization of a New Muscle Cell Line of Zebrafish (Danio rerio) as an In Vitro Model for Gene Expression Studies

2016 ◽  
Vol 27 (3) ◽  
pp. 166-173 ◽  
Author(s):  
Amit Kumar ◽  
Neha Singh ◽  
Mukunda Goswami ◽  
J. K. Srivastava ◽  
Akhilesh K. Mishra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Danio Rerio ◽  
In Vitro Model ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Vitro Model ◽  
Muscle Cell Line
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