An Improved Enzymatic-Ultraviolet Method for Determination of Uric Acid in Flours

1966 ◽  
Vol 49 (5) ◽  
pp. 899-902
Author(s):  
N P Sen ◽  
D Morison Smith

Abstract Main improvements in the enzymaticultraviolet method for uric acid in flours include elimination of the uricase precipitation by trichloroacetic acid, which was not reproducible, and destruction of uricaselike material in flour before extraction. The method is capable of measuring as low as 3.2 mg uric acid per 100 g flour and is applicable to a variety of cereal products such as rice, rye, corn and soya flours, as well as barley, oats, etc.


1963 ◽  
Vol 46 (3) ◽  
pp. 522-523
Author(s):  
Gin Farn ◽  
D Morison Smith

Abstract Improvements in the enzymatic-ultraviolet method for the determination of uric acid in flour include readjusting the sample size in relation to other reagents, using a more efficient extractant, and increasing the strengths of both the uricase and trichloroacetic acid solutions. Results indicate that the lower limit of the measurement is 10 mg per 100 g of flour.



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