Foodstuffs. Determination of ochratoxin A in cereals and cereal products

1998 ◽  
Keyword(s):  
Ochratoxin A ◽  
Cereal Products

Method for the Detection and Estimation of Ochratoxin A in Some Cereal Products

1967 ◽  
Vol 50 (2) ◽  
pp. 366-370
Author(s):  
P M Scott ◽  
T B Hand
Keyword(s):  
Ochratoxin A ◽  
Aqueous Methanol ◽  
Whole Wheat Flour ◽  
Cereal Products ◽  
Whole Wheat ◽  
Sample Extract ◽  
Final Sample ◽  
Aoac Method ◽  
Layer Chromatography

Abstract A method for the detection and semiquantitative estimation of ochratoxin A in flour and other cereal products is described which can be used in conjunction with analysis of the foodstuff for aflatoxins. The sample is extracted with aqueous methanol and n-hexane and the toxin is partitioned on a Celite column, as in the AOAC method for determination of aflatoxins in peanut products. Ochratoxin A is separated by thin layer chromatography and estimated by the intensity of fluorescence compared with that of a reference standard. By this procedure, 0.01 μg ochratoxin A can be detected in 10 μl final sample extract, corresponding to a detection limit of about 25 μg/kg cereal foodstuff. The method is applicable to whole wheat flour, corn meal, barley cereal, and rice cereal. Recoveries of 80—100% of added ochratoxin A were obtained

Ultrasonic Extraction with Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry for the Determination of Ochratoxin A in Processed Cereal Products

2014 ◽  
Vol 97 (5) ◽  
pp. 1384-1386 ◽  
Author(s):  
Kieu Thi Ngoc Nguyen ◽  
Dojin Ryu
Keyword(s):  
Mass Spectrometry ◽  
Ochratoxin A ◽  
Reliable Method ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Cereal Products ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Cereal Samples

Abstract A rapid, simple, and reliable method using ultra-performance LC/MS/MS (UPLC/MS/MS) was developed for determination of ochratoxin A (OTA) in processed cereal products. OTA was ultrasonically extracted from the sample with acetonitrile–water (80 + 20, v/v), and the extract was then injected into the UPLC/MS/MS system after filtration. The calibration curves had good linearity with coefficients of determination greater than 0.999. Recoveries of OTA were in the range of 90–104%. LOD and LOQ of OTA in samples were 0.6 and 2.0 ng/g, respectively, and no significant matrix effect was found. This method was applied to determine OTA in 25 oat-based cereal samples. OTA was detected in five samples (20%) in the range of 2.4 to 7.3 ng/g.

scholarly journals Determination of ochratoxin A in organic and non-organic cereals and cereal products from Spain and Portugal

2008 ◽  
Vol 107 (1) ◽  
pp. 525-530 ◽  
Author(s):  
C. Juan ◽  
J.C. Moltó ◽  
C.M. Lino ◽  
J. Mañes
Keyword(s):  
Ochratoxin A ◽  
Cereal Products

scholarly journals Liquid Chromatographic Determination of Ochratoxin A in Barley, Wheat Bran, and Rye by the AOAC/IUPAC/NMKL Method: NMKL Collaborative Study1

1996 ◽  
Vol 79 (5) ◽  
pp. 1102-1106 ◽  
Author(s):  
Kjell Larsson ◽  
Tord Möller ◽  
S Berg ◽  
I Berntsson ◽  
E Eklund ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Wheat Bran ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Cereal Products ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Standard Deviations ◽  
Liquid Chromatographic

Abstract In an inter laboratory study of the AOAC/lnternational Union of Pure and Applied Chemistry (IUPAC)/ Nordic Committee on Food Analysis (NMKL) liquid chromatographic method for ochratoxin A (OTA), 3 spiked samples each of wheat bran and rye and 3 naturally contaminated barley samples with expected OTA contents between 2 and 9 μg/kg were analyzed by 12 European laboratories. Mean recoveries ranged from 64 to 72%. Within-laboratory standard deviations were 21,17, and 12% for wheat bran, rye, and barley, respectively. Corresponding between-laboratory standard deviations were 23-28,20-28, and 18-31%. Horwitz ratio values ranged from 0.50 to 0.77. The method was accepted as an official NMKL method for determining OTA at ≥2 μg/kg in cereals and cereal products.

Rapid determination of ochratoxin A in cereals and cereal products by liquid chromatography

2004 ◽  
Vol 1046 (1-2) ◽  
pp. 127-131 ◽  
Author(s):  
J. Blesa ◽  
H. Berrada ◽  
J.M. Soriano ◽  
J.C. Moltó ◽  
J. Mañes
Keyword(s):  
Liquid Chromatography ◽  
Ochratoxin A ◽  
Rapid Determination ◽  
Cereal Products
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