Rapid Colorimetric Assay for Nitroglycerin, Suitable for Content Uniformity Testing

1970 ◽  
Vol 53 (3) ◽  
pp. 579-581
Author(s):  
Clyde E Wells ◽  
Harvey M Miller ◽  
Yvonne H Pfabe
Keyword(s):  
Standard Deviation ◽  
Colorimetric Assay ◽  
Content Uniformity ◽  
Colorimetric Determination ◽  
Isolation Method ◽  
Infrared Method ◽  
Content Uniformity Testing ◽  
Chromatographic Isolation ◽  
Column Chromatographic

Abstract The column chromatographic isolation method of Hohmann and Levine has been combined with a modified version of the colorimetric determination of Bell, resulting in a convenient assay for nitroglycerin in tablets. The method agrees closely with the AOAC infrared method and is sensitive enough for analysis of individual tablets (0.15 mg nitroglycerin). The standard deviation is 0.91% at the 1.2 mg level.

Rapid Colorimetric Assay of Trimethoprim and Sulfamethoxazole in Pharmaceuticals

1983 ◽  
Vol 66 (6) ◽  
pp. 1447-1449 ◽  
Author(s):  
Asis K Sanyal ◽  
Dinabandhu Laha
Keyword(s):  
Solvent Extraction ◽  
Colorimetric Assay ◽  
Pharmaceutical Preparations ◽  
Ion Pair ◽  
Pair Formation ◽  
Bromophenol Blue ◽  
Colorimetric Determination ◽  
Ion Pair Formation ◽  
Subsequent Measurement

Abstract A method is described for the direct colorimetric determination of trimethoprim and sulfamethoxazole in pharmaceutical preparations, without prior separation. Estimation of trimethoprim is based on its ion-pair formation with bromophenol blue and subsequent measurement of absorbance of the ionpair at 418 nm. Estimation of sulfamethoxazole is possible without removal of trimethoprim by solvent extraction.

Convenient and sensitive colorimetric determination of alendronate sodium with Ce4+-triggered oxidation of TMB

2020 ◽  
Vol 44 (30) ◽  
pp. 12962-12966
Author(s):  
Jing Sun ◽  
Rui Wang ◽  
Meng Xia ◽  
Shuyun Zhu ◽  
Xian-En Zhao
Keyword(s):  
Colorimetric Assay ◽  
Colorimetric Determination ◽  
Selective Detection ◽  
Alendronate Sodium ◽  
First Time

A facile colorimetric assay for the sensitive and selective detection of alendronate sodium has been developed based on Ce4+-triggered oxidation of TMB for the first time.

Determination of Orotic and Dihydroorotic Acids in Biological Fluids and Tissues

1975 ◽  
Vol 21 (3) ◽  
pp. 353-355 ◽  
Author(s):  
Leo Kesner ◽  
Frank L Aronson ◽  
Morris Silverman ◽  
Phillip C Chan
Keyword(s):  
Organic Acid ◽  
Orotic Acid ◽  
Colorimetric Assay ◽  
Biological Fluids ◽  
Acid Fraction ◽  
Chromatographic Procedure ◽  
Colorimetric Procedure ◽  
Column Chromatographic ◽  
Total Organic Acid

Abstract In commonly used procedures for colorimetric assay of orotic acid in biological materials, certain substances interfere, positively or negatively. Some that interfere negatively are ornithine, cysteine, citrulline, tyrosine, proline, hydroxyproline, and polyamines. Protein and interfering agents are easily removed by first isolating the total organic acid fraction. The orotic acid in this fraction can then be determined by an established colorimetric procedure. A rapid liquid—liquid column-chromatographic procedure is described, if analysis of both dihydroorotic and orotic acid is required.

Colorimetric Determination of Potassium in Plasma and Serum by Reflectance Photometry with a Dry-Chemistry Reagent

1992 ◽  
Vol 38 (7) ◽  
pp. 1371-1372 ◽  
Author(s):  
R H Ng ◽  
K M Sparks ◽  
B E Statland
Keyword(s):  
Reference Material ◽  
Standard Reference Material ◽  
Colorimetric Assay ◽  
Ion Selective Electrode ◽  
Colorimetric Determination ◽  
Selective Electrode ◽  
Flame Photometer ◽  
Near Patient Testing

Abstract We evaluated a colorimetric assay of potassium in plasma and serum with the Boehringer Mannheim Reflotron reflectance photometric analyzer, which is designed for near-patient testing in hospitals and physicians' offices. This potassium method does not require calibration or instrument maintenance by the operator. Analysis of 30 microL of plasma or serum takes approximately 140 s. Within-day imprecision (CV) was 1.0-1.2%. Total CVs over a 1-month period were 1.0-1.4%. Patients' results from the Reflotron correlated well with those from the IL 643 flame photometer and the Beckman Synchron CX3 ion-selective electrode methods. The accuracy of Reflotron values was also verified with Standard Reference Material 956 from the National Institute of Standards and Technology.

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