Determination of Procaine and Related Local Anesthetics. I. Partition Chromatographic Separation and Assay of Mixtures of Procaine with Tetracaine and with Propoxycaine

1975 ◽  
Vol 58 (1) ◽  
pp. 88-92
Author(s):  
Thomas D Doyle ◽  
Joseph B Proctor
Keyword(s):  
Local Anesthetics ◽  
Chromatographic Separation ◽  
Ion Pair ◽  
Sodium Bromide ◽  
Chromatographic System ◽  
Extraction Constants ◽  
Bromide Ion ◽  
Selection Of

Abstract Determination of ionization and extraction constants for procaine, tetracaine, and propoxycaine led to selection of a simple partition chromatographic system for separation and assay of mixtures of these anesthetics. A 65% solution of chloroform in isooctane elutes tetracaine or propoxycaine from a pH 4:sodium bromide column; procaine is retained and subsequently eluted by chloroform as the bromide ion-pair. The anesthetics are then determined spectrophotometrically. Results of assay of standard and commercial formulations are presented.

CHROMATOGRAPHIC SEPARATION OF URINARY CORTICOSTEROIDS

1960 ◽  
Vol XXXIV (III) ◽  
pp. 335-343 ◽  
Author(s):  
R. Emberland ◽  
K. F. Støa
Keyword(s):  
Propylene Glycol ◽  
Chromatographic Separation ◽  
Reducing Power ◽  
Phosphomolybdic Acid ◽  
Partition Chromatography ◽  
Chromatographic System ◽  
Specific Determination ◽  
The Right

ABSTRACT The usefulness of partition chromatography on cellulose columns has been tried in fractionation of urinary corticosteroids. As the chromatographic system toluene/propylene glycol has been used. During the first part of the experiments the determination of the corticosteroid content in the chromatographic fractions was carried out by measuring their reducing power against phosphomolybdic acid. Provided the right conditions were used, a satisfactory separation was obtained of the following steroids in mixture: 11-deoxycorticosterone, 11-dehydrocorticosterone, 11-deoxy-17-hydroxycorticosterone, corticosterone, cortisone, dihydrocortisone, tetrahydrocortisone, cortisol and tetrahydrocortisol. Aldosterone appeared in the same fractions as cortisone. By means of the borohydride-bismuthate reaction and a correction by the Allen formula in the subsequent spectrophotometry, an approximatively specific determination of a series of 17-hydroxycorticosteroids in urine extracts could be obtained.

Selection of Partition Chromatographic Systems from Distribution Diagrams: Determination of Dextromethorphan in Cough Sirups

1968 ◽  
Vol 51 (1) ◽  
pp. 191-199
Author(s):  
Thomas D Doyle ◽  
Joseph Levine
Keyword(s):  
General Procedure ◽  
Free Base ◽  
Standard Samples ◽  
Extraction Constants ◽  
Logarithmic Distribution ◽  
Selection Of

Abstract Ionization and extraction constants for dextromethorphan and 14 other alkoids are given. By constructing the logarithmic distribution diagrams for mixtures of these compounds, Celite partition chromatographic systems can easily be selected for their separation; both free base and ionpair extraction provide useful systems. A general procedure for the isolation of dextromethorphan from any of those alkoids with which it is currently formulated is presented; the results of analyses of standard samples and of commercial sirups are given. Some other separations do not appear to be feasible. However, the distribution diagrams clearly show the reasons for this fact

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