A validated direct thin-layer chromatographic separation and enantioselective determination of racemic centrally acting drugs using ion-pair and ligand-exchange chiral selectors: A thermodynamic study of complexation

2016 ◽  
Vol 29 (3) ◽  
pp. 176-183 ◽  
Author(s):  
Nahla N. Salama ◽  
Hala E. Zaazaa ◽  
Lobna M. Abd El Halim ◽  
Maissa Y. Salem ◽  
Laila E. Abd El Fattah
Keyword(s):  
Thin Layer ◽  
Chromatographic Separation ◽  
Ligand Exchange ◽  
Ion Pair ◽  
Thermodynamic Study ◽  
Chiral Selectors

Determination of Procaine and Related Local Anesthetics. I. Partition Chromatographic Separation and Assay of Mixtures of Procaine with Tetracaine and with Propoxycaine

1975 ◽  
Vol 58 (1) ◽  
pp. 88-92
Author(s):  
Thomas D Doyle ◽  
Joseph B Proctor
Keyword(s):  
Local Anesthetics ◽  
Chromatographic Separation ◽  
Ion Pair ◽  
Sodium Bromide ◽  
Chromatographic System ◽  
Extraction Constants ◽  
Bromide Ion ◽  
Selection Of

Abstract Determination of ionization and extraction constants for procaine, tetracaine, and propoxycaine led to selection of a simple partition chromatographic system for separation and assay of mixtures of these anesthetics. A 65% solution of chloroform in isooctane elutes tetracaine or propoxycaine from a pH 4:sodium bromide column; procaine is retained and subsequently eluted by chloroform as the bromide ion-pair. The anesthetics are then determined spectrophotometrically. Results of assay of standard and commercial formulations are presented.

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