Thin Layer Chromatographic Estimation of Available Lysine in Dried Milk Powder

1976 ◽  
Vol 59 (6) ◽  
pp. 1255-1257
Author(s):  
Shompa Datta ◽  
Salil C Datta
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rapid Method ◽  
Sulfonic Acid ◽  
Milk Powder ◽  
Pure Form ◽  
Trinitrobenzene Sulfonic Acid ◽  
Available Lysine ◽  
Layer Chromatography

Abstract A rapid method for the estimation of available lysine in dried milk powder has been developed. Samples were treated with 2,4,6-trinitrobenzene sulfonic acid and then hydrolyzed with 6N HCl. The hydrolysate was purified and subjected to ascending thin layer chromatography to isolate ε-trinitrophenyl lysine in pure form. The color was read at 346 nm in a spectrophotometer.

Subsidiary Colors in FD&C Blue No. 1

1969 ◽  
Vol 52 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Charles Stein
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Sulfonic Acid ◽  
Acid Group ◽  
Sulfonic Acid Group ◽  
Layer Chromatography

Abstract The subsidiary colors in commercial FD&C Blue No. 1 have been identified by thin layer chromatography. They consist of dyes formed by the loss of one sulfonic acid group or of one sulfonated benzyl group from FD&C Blue No. 1.

Collaborative Study of a Rapid Method for Multiple Chlorinated Pesticide Residues in Small Fruits

1966 ◽  
Vol 49 (2) ◽  
pp. 468-472
Author(s):  
Arthur W Davidson
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rapid Method ◽  
Pesticide Residues ◽  
Collaborative Study ◽  
Layer Chromatography

Abstract Twelve collaborators from six laboratories used gas-liquid and thin layer chromatography to measure BHC, p,p′-DDT, and endrin residues on apricots and strawberries. Recoveries for both crops by gas chromatography averaged: BHC, 104 ± 23 %; p,p′-DDT, 102 ± 10%; endrin, 96 ±14%. Recoveries for both crops by thin layer chromatography averaged: BHC, 98 ± 29%; p,p′-DDT, 97 ± 18%; and endrin, 96 ± 16%.

Rapid assay of poly(ADP-ribose) glycohydrolase

1987 ◽  
Vol 65 (7) ◽  
pp. 668-673 ◽  
Author(s):  
Luc Ménard ◽  
Guy G. Poirier
Keyword(s):  
Thin Layer ◽  
Enzymatic Activity ◽  
Affinity Chromatography ◽  
Thin Layer Chromatography ◽  
Rapid Method ◽  
The Other ◽  
High Yield ◽  
Rapid Assay ◽  
Layer Chromatography

We have developed a rapid, highly reproducible assay to determine poly(ADP-ribose) glycohydrolase activity which measures directly the appearance of the reaction product. We also analysed the majority of different techniques which are used to determine poly(ADP-ribose) glycohydrolase activity and found that the apparent activity can vary extensively depending on the method used. Thin-layer chromatography using PEI-F–cellulose was the only method which evaluated directly the specific release of ADP-ribose; by comparison with this method, the other procedures gave an over- or under-estimation of 2- to 10-fold of the enzymatic activity. A rapid method of affinity chromatography has also been developed to synthesize and purify in high yield poly(ADP-ribose) (35% conversion of 1 mM NAD to poly (ADP-ribose)).

The anthocyanins of Dicentra (Fumariaceae)

1970 ◽  
Vol 48 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Dianne Fahselt
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rapid Method ◽  
Interspecific Hybrids ◽  
Anthocyanin Pigments ◽  
Layer Chromatography

The main anthocyanin pigments of the genus Dicentra have been characterized through the use of spectroscopy and chromatography. This combination of techniques, particularly suited to anthocyanins, has demonstrated that all are glycosides of cyanidin (3,5,7,3′,4′-pentahydroxyflavylium) and that none are acylated. The simplest, found in several species, is cyanidin-3-monoglucoside, while the most complex is cyanidin-3-rhamnoglucosido-7-xyloside. This triglycoside is present in only one species as well as in certain experimental interspecific hybrids, and this is its first reported occurrence in nature. A rapid method is described for identification of anthocyanin sugars using thin-layer chromatography.

Sign in / Sign up

Export Citation Format

Share Document