Subsidiary Colors in FD&C Blue No. 1

1969 ◽  
Vol 52 (1) ◽  
pp. 34-40 ◽  
Author(s):  
Charles Stein
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Sulfonic Acid ◽  
Acid Group ◽  
Sulfonic Acid Group ◽  
Layer Chromatography

Abstract The subsidiary colors in commercial FD&C Blue No. 1 have been identified by thin layer chromatography. They consist of dyes formed by the loss of one sulfonic acid group or of one sulfonated benzyl group from FD&C Blue No. 1.

Thin Layer Chromatographic Estimation of Available Lysine in Dried Milk Powder

1976 ◽  
Vol 59 (6) ◽  
pp. 1255-1257
Author(s):  
Shompa Datta ◽  
Salil C Datta
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Rapid Method ◽  
Sulfonic Acid ◽  
Milk Powder ◽  
Pure Form ◽  
Trinitrobenzene Sulfonic Acid ◽  
Available Lysine ◽  
Layer Chromatography

Abstract A rapid method for the estimation of available lysine in dried milk powder has been developed. Samples were treated with 2,4,6-trinitrobenzene sulfonic acid and then hydrolyzed with 6N HCl. The hydrolysate was purified and subjected to ascending thin layer chromatography to isolate ε-trinitrophenyl lysine in pure form. The color was read at 346 nm in a spectrophotometer.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

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