High Pressure Liquid Chromatographic Determination of Phenol in Honey

1981 ◽  
Vol 64 (2) ◽  
pp. 337-339
Author(s):  
Peter Sporns
Keyword(s):  
High Pressure ◽  
Mobile Phase ◽  
Silicic Acid Column ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Peak Height ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is described for determining phenol in honey by using high pressure liquid chromatography (HPLC). An internal standard, 2-phenylethanol, was added to honey which was steam-distilled and chromatographed on a 25 cm × 3.2 mm id Spherisorb 5 µm silicic acid column using water as the mobile phase. Absorbance was monitored at 195 nm. Using a mixed standard of known concentration and peak height measurements, the amount of phenol in the honey could be quantitated. Recovery of added phenol was checked at levels from 0.1 to 33 ppm.

High Pressure Liquid Chromatographic Determination of Supplemental Methionine in Poultry Premix

1982 ◽  
Vol 65 (1) ◽  
pp. 62-65
Author(s):  
Dubravka Matešič
Keyword(s):  
High Pressure ◽  
Mobile Phase ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Buffer Solution ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Supplemental methionine was extracted from a feed sample with O.IM HCI and separated by reverse phase or ion-exchange high pressure liquid chromatography and isocratic elution with KH2PO4 buffer solution as the mobile phase. Methionine was detected at 205 nm. The most reliable results were obtained by using reverse phase chromatography, 0.05M KH2PO4 buffer at pH 2.6 as mobile phase, and tyrosine as internal standard.

High Pressure Liquid Chromatographic Determination of Theobromine and Caffeine in Cocoa and Chocolate Products: Collaborative Study

1980 ◽  
Vol 63 (3) ◽  
pp. 591-594
Author(s):  
Wesley R kreiser ◽  
Robert A Martin ◽  
◽  
R Bigornia ◽  
R Bond ◽  
...  
Keyword(s):  
High Pressure ◽  
Petroleum Ether ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Peak Height ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
The Mean ◽  
Liquid Chromatographic

Abstract Four duplicate samples of cocoa-containing materials, a practice sample, and standards were submitted to the collaborators for theobromine and caffeine analysis by HPLC. In the method the samples are defatted with petroleum ether, and dried. The fat-free residue is then extracted with water and an aliquot is injected into the chromatograph. Compounds are quantitated by comparison with internal or external standards, either by peak height or peak area. Results for all the analyses showed that few of the values were more than 2 standard deviations from the mean. The method has been adopted as official first action.

High Pressure Liquid Chromatographic Determination of Physostigmine Salicylate and Physostigmine Sulfate in Liquids and Ointments: Collaborative Study

1982 ◽  
Vol 65 (1) ◽  
pp. 132-137
Author(s):  
Norlin W Tymes ◽  
◽  
G Briguglio ◽  
C Corcoran ◽  
R Everett ◽  
...  
Keyword(s):  
High Pressure ◽  
Collaborative Study ◽  
Internal Standard ◽  
Pharmaceutical Formulations ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Results of 11 laboratories are presented for the collaborative study of a proposed method for the quantitative reverse phase high pressure liquid chromatographic (HPLC) determination of physostigmine salicylate and physostigmine sulfate in pharmaceutical formulations. The samples consisted of commercial solution, injection, and ointment preparations, each containing one of the physostigmine salts. The physostigmine salt is extracted from ointments with acetonitrile after the ointment is dissolved in hexane. Liquid preparations are diluted directly. Physostigmine is determined at 254 nm on a C18 column by comparison with a physostigmine standard. Flurazepam hydrochloride is the internal standard. The method has been adopted official first action for the solution dosage form.

High Pressure Liquid Chromatographic Determination of Sorbitol in Bulk Sorbitol

1980 ◽  
Vol 63 (3) ◽  
pp. 664-666 ◽  
Author(s):  
J DOkladalova ◽  
A Y Barton ◽  
E A Mackenzie
Keyword(s):  
High Pressure ◽  
Refractive Index ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
High Pressure Liquid Chromatographic ◽  
Column Eluate ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Refractive Index Detector

Abstract An HPLC procedure for determining sorbitol in bulk sorbitol is described. An Aminex HPX-87 column (Bio-Rad) is used with water as a mobile phase and with a refractive index detector to monitor column eluate. Sorbitol is separated from pentaerythritol, erythritol, ribitol, ethylene glycol, propylene glycol, arabitol, galactitol, mannitol, iditol, and other carbohydrates. The precision of the sorbitol determination, characterized by a 95% confidence interval, corresponds to ±1.22%.

High Pressure Liquid Chromatographic Determination of 3,5-Dimethyl-4-(Methylthio) phenyl Methylcarbamate in Formulations

1979 ◽  
Vol 62 (1) ◽  
pp. 5-7
Author(s):  
Martha Fuzesi
Keyword(s):  
High Pressure ◽  
Quantitative Measurement ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Pesticide Formulations ◽  
Liquid Chromatographic Determination ◽  
Commercial Pesticide ◽  
Liquid Chromatographic

Abstract Methiocarb (3,5 - dimethyl - 4 - (methylthio) - phenyl methylcarbamate) is extracted from commercial pesticide formulations with methanol, and carbaryl is added as an internal standard. The sample is chromatographed by reverse phase high pressure liquid chromatography, with ultraviolet detection at 254 nm. Peak height ratios are used for quantitative measurement. The method is rapid and accurate, and recoveries from laboratory-prepared formulations averaged 100.8%.

High Pressure Liquid Chromatographic Determination of Pentachlorophenol by Using Paired Ion Chromatography Reagents

1979 ◽  
Vol 62 (5) ◽  
pp. 1004-1006
Author(s):  
Elmer H Hayes
Keyword(s):  
High Pressure ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
High Pressure Liquid Chromatographic ◽  
Steel Column ◽  
Stainless Steel Column ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high pressure liquid chromatographic (HPLC) method for determining pentachlorophenol in formulations is described. Samples containing pentachlorophenol are accurately weighed in suitable volumetric flasks and diluted with dioxane. The sample is then injected onto a stainless steel column containing μBondapak C18. The mobile phase is 60% methanol/PIC A and 40% water/PIC A. This method is simple and eliminates many of the extractions required in other methods of analysis.

Reverse Phase High Pressure Liquid Chromatographic Determination of Arprinocid in Animal Feeds

1982 ◽  
Vol 65 (1) ◽  
pp. 43-47
Author(s):  
Virginia A Thorpe
Keyword(s):  
High Pressure ◽  
Chromatographic Determination ◽  
Peak Height ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Simple Method ◽  
Working Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple method is presented for determination of arprinocid in finished feeds by reverse phase high pressure liquid chromatography. The sample is extracted with 95% DMF, the major feed interferences are removed by alumina chromatography, and arprinocid is separated from the remaining interferences on the HPLC column. The peak height detected at 254 nm can be quantitated by direct comparison with the working standard.

Elimination of Sodium Chloride Interference During High Pressure Liquid Chromatographic Determination of Sugars

1983 ◽  
Vol 66 (1) ◽  
pp. 197-198
Author(s):  
Jonathan W Devries ◽  
Henry L Chang ◽  
John C Heroff ◽  
Kevin D Johnson
Keyword(s):  
High Pressure ◽  
Sodium Chloride ◽  
Mobile Phase ◽  
Chromatographic Determination ◽  
Food Samples ◽  
Hplc Separation ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A method is described to eliminate the potential interference of sodium chloride found in some food samples, which occurs during high pressure liquid chromatographic (HPLC) analysis of sugars, using certain bonded aminopropyl columns. The column is tested, and if an interference is present, it is removed by washing the column with a solution of tetraethylenepentamine in mobile phase. HPLC separation and quantitation of the sugars are essentially the same as before washing; however, sodium chloride no longer interferes with any of the sugars being analyzed.

Simultaneous high-pressure liquid-chromatographic determination of some anticonvulsants in serum.

1976 ◽  
Vol 22 (1) ◽  
pp. 25-31 ◽  
Author(s):  
R F Adams ◽  
F L Vandemark
Keyword(s):  
High Pressure ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Serum Samples ◽  
Reverse Phase ◽  
High Pressure Liquid Chromatographic ◽  
Peak Areas ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract We describe procedures for simultaneously determining some anticonvulsants (phenobarbital, diphenylhydantoin, primidone, ethosuximide, methsuximide, carbamazepine) in serum by high-pressure liquid chromatography. The drugs, together with an internal standard, phenacetin, are adsorbed from serum onto charcoal and eluted from it with organic solvent. The eluate is analyzed isocratically on a reverse-phase column with a mobile phase consisting of acetonitrile/water (17/83 by volume). The eluted drugs are detected by their absorption at 195 nm, and quantities estimated from their peak areas as compared with those of extracted standards. Results are reproducible to about 6%. Sensitivities, for 0.5-ml serum samples, of 0.1 mg/liter for all the drugs analyzed except ethosuximide (0.5 mg/liter) are attained routinely. Correlation of results with gas chromatography was 0.912 for phenobarbital, 0.982 for diphenylhydantoin, 0.886 for primidone, and 0.966 for ethosuximide. Amobarbital and secobarbital interfere with the analysis; chlordiazepoxide, methaqualone, salicylate, diazepam, and oxazepam do not. Including extraction, analysis time for a single sample is 20 min.

High Pressure Liquid Chromatographic Determination of Difenzoquat in Formulations

1980 ◽  
Vol 63 (3) ◽  
pp. 647-649
Author(s):  
Carla Barry ◽  
Richard K Pike
Keyword(s):  
High Pressure ◽  
Quantitative Measurement ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
High Pressure Liquid Chromatographic ◽  
Pesticide Formulations ◽  
Liquid Chromatographic Determination ◽  
Commercial Pesticide ◽  
Liquid Chromatographic

Abstract Difenzoquat (l,2-dimethyl-3,5-diphenylpyrazolium ion) as its methyl sulfate is extracted from commercial pesticide formulations with acetonitrile-water (1+), with acetophenone added as internal standard. The sample is chromatographed by reverse phase high pressure liquid chromatography with ultraviolet detection at 280 nm. Peak area ratios are used for quantitative measurement. The method is simple, rapid, and direct, and its applicability to other quaternary ammonium herbicides is discussed.

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