The dietary effect of milk sphingomyelin on the lipid metabolism of obese/diabetic KK-Aymice and wild-type C57BL/6J mice

Purified milk sphingomyelin (SM) was obtained from lipid concentrated butter serum (LC-BS) by successive separations involving solvent fractionation, selective saponification, and silicic acid column chromatography.

Studies on the Lipid Classes and Fatty Acid Compositions of Petuli (Trewia nudiflora Linn.) Seed oil

Studies were carried out on the lipid classes and fatty acid compositions of petuli seed oil. It was observed that petuli seed grown under the soil and climatic condition of Bangladesh contains about 22% pale yellow coloured oil. The total lipids were fractionated into three major lipid classes, neutral lipid glyco lipid and phospho lipid by silicic acid column chromatography. The neutral lipid was accounted to 92.5% of the total weight of the lipid applied. The oil was also fractionated into mono, di and triglyceride by silicic acid column chromatography. The triglyceride was counted for over 90% of the total weight of the oil. The fatty acid compositions of the oil were analyzed by Gas liquid Chromatography and found the major fatty acid ?-elaeostearic acid 38.50%, oleic acid 34.35%, linoleic acid 26.15% and small amount of arachidic acid 1%. DOI: http://dx.doi.org/10.3329/bjsir.v47i1.10716 Bangladesh J. Sci. Ind. Res. 47(1), 105-108, 2012

Evidence for the Presence of Glycerophosphonolipids in the Land Snail Eobania vermiculata

Total phosphonolipids from the land snail have been iso­lated by thin-layer chromatography (TLC) using the sol­vent system methanol-water (2:1) and have been sub­jected to silicic acid column chromatography. The fraction eluted with 40% methanol in chloroform has been ob­tained, the single species isolated by these means has been identified as the phosphono analog of phosphatidyl choline and has been fully characterized by TLC, IR spectroscopy, elemental analysis and phosphono-phosphorus determina­tions.

Notizen: Isolation of Beef Brain Phosphonolipids by Thin Layer Chromatography: Their Identification and Silicic Acid Column Chromatographic Separation

The isolation and identification of the phosphonolipids from beef brain - right lobe, left lobe and cerebellum - is herein reported. The phosphonolipids were isolated by preparative thin layer chromatography (TLC) using the solvent system methanol/water (2:1, v/v) and identified preliminarily by TLC. The isolated phosphonolipids were subjected to silicic acid column chromatographic separation and the thus separated phosphonolipids were identified by TLC, nitrogen-phosphorus determinations and IR spectroscopy.