Stability-Indicating Liquid Chromatographic Determination of Alpha-Ionone in Toothpaste

1988 ◽  
Vol 71 (1) ◽  
pp. 36-37
Author(s):  
Ramesh J Trivedi
Keyword(s):  
Standard Deviation ◽  
Chromatographic Method ◽  
Cost Effective ◽  
Chromatographic Determination ◽  
Average Recovery ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple, sensitive, and rapid liquid chromatographic method for quantitating α-ionone in toothpaste at levels of 20 ppm in the presence of large amounts of flavor has been developed. The method is accurate, precise, cost-effective, and specific for α-ionone. Average recovery of a laboratory-prepared sample was 99.0% with the relative standard deviation was 1.29% (n = 6).

Liquid Chromatographic Determination of Dicloxacillin Preparations: Interlaboratoiy Study

1993 ◽  
Vol 76 (5) ◽  
pp. 1143-1145 ◽  
Author(s):  
Mei-Chich Hsu ◽  
Weng F Huang
Keyword(s):  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Performance Requirements ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Repeatability And Reproducibility ◽  
The Individual ◽  
Liquid Chromatographic

Abstract A previously published liquid chromatographic method proposed for the analysis of dicloxacillin preparations was subjected to an interlaboratory study. The method is rigorously defined in terms of performance requirements, yet allows a degree of flexibility to the individual analyst. Eight laboratories participated in a study to analyze 3 samples in duplicate. Estimates for the repeatability and reproducibility of the method, expressed as relative standard deviations of the results of the determination of dicloxacillin preparations, were <0.57 and 2.56%, respectively.

Liquid Chromatographic Determination of the Anticoccidial Drug Halofuginone Hydrobromide in Eggs

1995 ◽  
Vol 78 (1) ◽  
pp. 37-40 ◽  
Author(s):  
David C Holland ◽  
Robert K Munns ◽  
Jose E Roybal ◽  
Jeffrey A Hurlbut ◽  
Austin R Long
Keyword(s):  
Standard Deviation ◽  
Mobile Phase ◽  
Ammonium Acetate ◽  
Chromatographic Determination ◽  
Free Base ◽  
Average Recovery ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for the determination of 5-100 ppb halofuginone hydrobromide (HFG) in eggs. HFG as the free base is extracted from eggs with ethyl acetate. The extract is cleaned up on an acidic Celite 545 column. A Waters C18 column is used for LC separation with UV determination at 243 nm. The isocratic mobile phase is a mixture of water-acetonitrile-ammonium acetate buffer (12 + 5 + 3) and acetic acid. The interassay average recovery from eggs was 90.4%, with a standard deviation of 5.11 and a relative standard deviation of 5.65%.

scholarly journals Liquid Chromatographic Determination of Methyl Anthranilate in Artificially Flavored Nonalcoholic Beverages

2001 ◽  
Vol 84 (2) ◽  
pp. 493-497 ◽  
Author(s):  
Richard D Thompson ◽  
John T Quaife
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Methyl Anthranilate ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Nonalcoholic Beverages

Abstract A liquid chromatographic method was developed that provides a simple and rapid means of determining methyl anthranilate (MA) in carbonated and noncarbonated, artificial grape-flavored, nonalcoholic beverages. The proposed procedure, which was applied to 12 different products, uses a Nova-Pak C18 column, a mobile phase containing acetonitrile–0.025M KH2PO4 (40 + 60), pH 3.00, and UV detection at 220 nm. Assay values ranged from 0.35 to 16.6 Μg MA/mL. The intralaboratory precision (relative standard deviation) for the products ranged from 0.51 to 2.23% (n = 5), and recoveries via fortification ranged from 83.6 to 102.4%. The limits of quantitation and detection were 0.00417 and 0.00125 μg/mL, respectively, and the analyte response was linear over a 100-fold concentration range (0.0001–0.01 mg/mL).

Liquid Chromatographic Determination of Carminic Acid in Yogurt

1989 ◽  
Vol 72 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Mercedes Jalón ◽  
Majesús Peńa ◽  
Julián C Rivas
Keyword(s):  
Chromatographic Determination ◽  
Carminic Acid ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Array Detection ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reverse-phase liquid chromatographic method is described for the determination of carminic acid in yogurt. A C18 column is used with acetonitrile-1.19M formic acid (19 + 81) as mobile phase and diode array detection. Sample preparation includes deproteinization with papain and purification in a polyamide column. The relative standard deviation for repeated determinations of carminic acid in a commercial strawberry-flavored yogurt was 3.0%. Recoveries of carminic acid added to a natural-flavored yogurt ranged from 87.2 to 95.3% with a mean of 90.2%. The method permits measurement of amounts as low as 0.10 mg/kg.

Gas-Liquid Chromatographic Determination of T-2 Toxin in Plasma

1983 ◽  
Vol 66 (4) ◽  
pp. 909-912 ◽  
Author(s):  
Steven P Swanson ◽  
Venkatachalam Ramaswamy ◽  
Val R Beasley ◽  
William B Buck ◽  
Harold H Burmeister
Keyword(s):  
Chromatographic Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Aqueous Sodium Hydroxide ◽  
Chromatographic Determination ◽  
Florisil Column ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract The gas-liquid chromatographic method for the determination of T-2 toxin in plasma is described. The toxin is extracted with benzene, washed with aqueous sodium hydroxide, and chromatographed on a small Florisil column; the heptafluorobutyryl derivative is prepared by reaction with heptafluorobutyrylimidazole. The T-2 HFB derivative is chromatographed onOV-1 at 230°C and measured with an electron capture detector. Iso-T-2, an isomer of T-2 toxin, is added to samples as an internal standard before extraction. Recoveries averaged 98.0 ± 5.5% at levels ranging from 50 to 1000 ng/m L. The limit of detection is 25 ng/mL.

Liquid Chromatographic Determination of Morphine Sulfate and Some Contaminants in Injections and Bulk Drug Material: Collaborative Study

1988 ◽  
Vol 71 (5) ◽  
pp. 1046-1048
Author(s):  
Ada C Bello ◽  
Rita K Jhangiani
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Morphine Sulfate ◽  
System Suitability ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Bulk Drug ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the assay of morphine sulfate and some preservatives and impurities in the bulk drug and in injections has been developed and collaboratively studied in 8 laboratories. Each collaborator analyzed 5 samples: 1 bulk drug, 3 different concentrations of injectable dosages, and 1 prepared mixture containing, in addition to morphine sulfate, phenol, 2-mercaptobenzothiazole, and pseudomorphine. The proposed method quantitates morphine sulfate and resolves the other components for identification using a Clg reverse-phase column with a mobile solvent containing 240 mL methanol, 720 mL 0.005M 1-heptanesulfonic acid Na salt, and 10 mL acetic acid. Samples are prepared by direct dilution with mobile solvent minus 1-heptanesulfonic acid. All collaborators met system suitability requirements and performed the analysis without difficulty. No outliers were found when data were analyzed by the Dixon, Grubbs, double Grubbs, and Cochran tests. Relative standard deviations between laboratories (RSDR) for duplicate determinations of morphine sulfate ranged from 1.4 to 2.1%. Mean morphine sulfate recoveries for the bulk drug and the prepared mixture were 100.8 and 100.4%, respectively. The method has been approved interim official first action.

High Performance Liquid Chromatographic Determination of Primidone in Tablets

1982 ◽  
Vol 65 (5) ◽  
pp. 1063-1065
Author(s):  
Stanley E Roberts
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Average Recovery ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high performance liquid chromatographic (HPLC) method is described for the quantitative determination of primidone in tablets. A ground tablet sample is diluted directly in the mobile phase, at a concentration of about 1 mg/mL of primidone, mixed and deaerated, and filtered. The resulting solution is then quantitated by HPLC. The average spike recoveries for the 50 mg and 250 mg tablets were 101.2% and 99.0%, respectively. The average recovery for an authentic mixture formulated at the 250 mg level was 100.1% with a relative standard deviation of 0.45%.

scholarly journals Liquid Chromatographic Determination of the Glycoalkaloids α-Solanine and α-Chaconine in Potato Tubers: NMKL Interlaboratory Study

1997 ◽  
Vol 80 (3) ◽  
pp. 549-554 ◽  
Author(s):  
Karl-Erik Hellenás ◽  
Carina Branzell ◽  
H Poutanen ◽  
T Suortti ◽  
R Kaario ◽  
...  
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Potato Tubers ◽  
Photometric Detection ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Twelve laboratories participated in a collaborative study to evaluate precision parameters of a liquid chromatographic method for analysis of the glycoalkaloids α-solanine and α-chaconine in potato tubers. Samples consisted of frozen potato tuber homogenates distributed as 3 blind duplicates and 3 split-level pairs. The analytical method included aqueous extraction, workup on disposable solidphase extraction cartridges, and reversed-phase chromatography with photometric detection at 202 nm. Results for α-solanine and α-chaconine were received from 10 and 9 laboratories, respectively. Relative standard deviations for reproducibilo ity for α-solanine and α-chaconine were similar, ranging from 8 to 13% in the applied concentration range of 12 to 260 mg/kg fresh weight.

Liquid Chromatographic Determination of Propoxur in Technical and Formulated Products: Collaborative Study

1984 ◽  
Vol 67 (3) ◽  
pp. 497-499
Author(s):  
Stephen C Slahck ◽  
◽  
J B Audino ◽  
O O Bennett ◽  
B D Folsom ◽  
...  
Keyword(s):  
Chromatographic Method ◽  
Collaborative Study ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Wettable Powder ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the determination of propoxur in technical and formulated products has been subjected to a collaborative study with 8 participating collaborators. Formulations are extracted with acetonitrile and analyzed by reverse phase chromatography, with n-butyrophenone as an internal standard. Collaborators were furnished with samples of technical, 70% wettable powder, 1.5 emulsifiable, and 2% bait. Coefficient of variation values obtained on the 4 samples were 0.34, 0.68, 3.25, and 5.41%, respectively. The method has been adopted official first action.

Liquid Chromatographic Method for Determination of Explosives Residues in Soil: Collaborative Study

1990 ◽  
Vol 73 (4) ◽  
pp. 541-552 ◽  
Author(s):  
Christopher F Bauer ◽  
Stephan M Koza ◽  
Thomas F Jenkins
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Contaminated Soils ◽  
Chromatographic Method ◽  
Collaborative Study ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Explosives Residues ◽  
Liquid Chromatographic

Abstract A collaborative study of a sonic extraction/liquid chromatographic method for determining nitroaromatic and nitramlne explosives In soil was conducted at 8 participating laboratories. Analytes HMX, RDX, TNB, DNB, tetryl, TNT, and 2,4-DNT were measured In duplicate for 4 field-contaminated soils and 4 spiked standard-matrix soils. Concentrations ranged from detection limits of about 1 μg/g to nearly 1000 μg/g. Results were evaluated with and without data Identified as outliers, which were often caused by electronic integrator miscalculation of chromatographic peak response. When outliers are excluded, method repeatability (within-laboratory relative standard deviation) for all analytes except tetryl Is less than 5% for spiked soils and less than 18% for fieldcontaminated soils. Relative standard deviation generally decreases as analyte concentration Increases. Reproducibility (between-laboratory relative standard deviation), except for tetryl and DNT, Is less than 7% for spiked soils and 26% for fleld-contamlnated soils. Thus, collaborators have nearly equivalent performance on spiked samples. For fleld-contamlnated soils, some additional Imprecision seems to result from the variability of extraction recoveries. Analyte recoveries from spiked soils are 95-97% for HMX, RDX, TNT, and DNT (similar to recoveries from aqueous samples); 92-93% for DNB and TNB; and 70% for tetryl. Poor results for tetryl (due to thermal degradation) are correctable If sonic bath temperatures are maintained near ambient. The method has been approved Interim official first action by AOAC.

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