scholarly journals Liquid Chromatographic Determination of Methyl Anthranilate in Artificially Flavored Nonalcoholic Beverages

2001 ◽  
Vol 84 (2) ◽  
pp. 493-497 ◽  
Author(s):  
Richard D Thompson ◽  
John T Quaife
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Methyl Anthranilate ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Nonalcoholic Beverages

Abstract A liquid chromatographic method was developed that provides a simple and rapid means of determining methyl anthranilate (MA) in carbonated and noncarbonated, artificial grape-flavored, nonalcoholic beverages. The proposed procedure, which was applied to 12 different products, uses a Nova-Pak C18 column, a mobile phase containing acetonitrile–0.025M KH2PO4 (40 + 60), pH 3.00, and UV detection at 220 nm. Assay values ranged from 0.35 to 16.6 Μg MA/mL. The intralaboratory precision (relative standard deviation) for the products ranged from 0.51 to 2.23% (n = 5), and recoveries via fortification ranged from 83.6 to 102.4%. The limits of quantitation and detection were 0.00417 and 0.00125 μg/mL, respectively, and the analyte response was linear over a 100-fold concentration range (0.0001–0.01 mg/mL).

Stability-Indicating Liquid Chromatographic Determination of Alpha-Ionone in Toothpaste

1988 ◽  
Vol 71 (1) ◽  
pp. 36-37
Author(s):  
Ramesh J Trivedi
Keyword(s):  
Standard Deviation ◽  
Chromatographic Method ◽  
Cost Effective ◽  
Chromatographic Determination ◽  
Average Recovery ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple, sensitive, and rapid liquid chromatographic method for quantitating α-ionone in toothpaste at levels of 20 ppm in the presence of large amounts of flavor has been developed. The method is accurate, precise, cost-effective, and specific for α-ionone. Average recovery of a laboratory-prepared sample was 99.0% with the relative standard deviation was 1.29% (n = 6).

scholarly journals Liquid Chromatographic Determination of Scopolamine, Hyoscyamine, and Phenobarbital in Tablets

1997 ◽  
Vol 80 (2) ◽  
pp. 331-334 ◽  
Author(s):  
Susan Ting
Keyword(s):  
Mobile Phase ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Scopolamine Hydrobromide ◽  
Acid Sodium Salt ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method using a reversed- phase C18 column and octanesulfonic acid sodium salt-methanol as the mobile phase was developed for the simultaneous determination of phenobarbi- tal, scopolamine, and hyoscyamine in tablets. The mixture of the 3 drugs was resolved in <8 min. Detector responses were linear for 10 μL injections of the following: scopolamine hydrobromide, 8.25-206.3 μg/mL; hyoscyamine sulfate, 15.01-750.76 μg/mL; and phenobarbital, 250-751 μg/mL. Recoveries from tablets were 100.8% for scopolamine hydrobromide, 100.1% for hyoscyamine sulfate, and 100.3% for phenobarbital. Replicate injections of scopolamine hydrobromide, hyoscyamine sulfate, and phenobarbital gave an overall relative standard deviation of <1.0% (n = 10). The method detected as little as 3.3 ng scopolamine hydrobromide.

Liquid Chromatographic Determination of Dicloxacillin Preparations: Interlaboratoiy Study

1993 ◽  
Vol 76 (5) ◽  
pp. 1143-1145 ◽  
Author(s):  
Mei-Chich Hsu ◽  
Weng F Huang
Keyword(s):  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Performance Requirements ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Repeatability And Reproducibility ◽  
The Individual ◽  
Liquid Chromatographic

Abstract A previously published liquid chromatographic method proposed for the analysis of dicloxacillin preparations was subjected to an interlaboratory study. The method is rigorously defined in terms of performance requirements, yet allows a degree of flexibility to the individual analyst. Eight laboratories participated in a study to analyze 3 samples in duplicate. Estimates for the repeatability and reproducibility of the method, expressed as relative standard deviations of the results of the determination of dicloxacillin preparations, were <0.57 and 2.56%, respectively.

Liquid Chromatographic Determination of Barbaloin (Aloin) in Foods

1985 ◽  
Vol 68 (3) ◽  
pp. 493-494
Author(s):  
Masatoshi Yamamoto ◽  
Masaaki Ishikawa ◽  
Toshio Masui ◽  
Hiroyuki Nakazawa ◽  
Yozo Kabasawa
Keyword(s):  
Liquid Chromatography ◽  
Present Method ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Food Samples ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple and rapid liquid chromatographic method is described for the determination of barbaloin (aloin, 10-D-gIucopyranosyl-l,8-dihydroxy- 3-(hydroxymethyI)-9(10#)-anthracenone) in foods. Barbaloin is extracted with water from foods containing aloe and the extract is cleaned up on a disposable cartridge by using methanol-water (55 + 45) as eluant. The eluted barbaloin is separated by liquid chromatography on a YMC A-302 column with methanol-water (50 + 50) mobile phase, and detected at 293 nm. Recoveries of barbaloin added to foods at the levels of 0.05 and 0.50 mg/g were 94.4-100%. Assay results for commercial food samples indicated that the present method is applicable to a variety of foods supplemented with aloe.

Liquid Chromatographic Determination of Sucralfate in Dental Cream

1992 ◽  
Vol 75 (5) ◽  
pp. 809-811 ◽  
Author(s):  
Robert J Gargiullo
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
Anion Exchange Column ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Tetrabutylammonium Hydrogen Sulfate ◽  
Refractive Index Detection

Abstract A simple, rapid liquid chromatographic method is described for quantitative determination of sucralfate in dental cream. Analyses were performed on a weak anion exchange column with 0.6M ammonium sulfate-0.005M tetrabutylammonium hydrogen sulfate as the mobile phase and refractive index detection. The method was linear over a range of 0.0 to 20.0% sucralfate in dental cream. The coefficient of variation was 3.2%. Standard recoveries were concentration dependent and ranged from 97.2 to 104.9%.

Liquid Chromatographic Determination of Carminic Acid in Yogurt

1989 ◽  
Vol 72 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Mercedes Jalón ◽  
Majesús Peńa ◽  
Julián C Rivas
Keyword(s):  
Chromatographic Determination ◽  
Carminic Acid ◽  
Reverse Phase ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Array Detection ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reverse-phase liquid chromatographic method is described for the determination of carminic acid in yogurt. A C18 column is used with acetonitrile-1.19M formic acid (19 + 81) as mobile phase and diode array detection. Sample preparation includes deproteinization with papain and purification in a polyamide column. The relative standard deviation for repeated determinations of carminic acid in a commercial strawberry-flavored yogurt was 3.0%. Recoveries of carminic acid added to a natural-flavored yogurt ranged from 87.2 to 95.3% with a mean of 90.2%. The method permits measurement of amounts as low as 0.10 mg/kg.

Gas-Liquid Chromatographic Determination of T-2 Toxin in Plasma

1983 ◽  
Vol 66 (4) ◽  
pp. 909-912 ◽  
Author(s):  
Steven P Swanson ◽  
Venkatachalam Ramaswamy ◽  
Val R Beasley ◽  
William B Buck ◽  
Harold H Burmeister
Keyword(s):  
Chromatographic Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Aqueous Sodium Hydroxide ◽  
Chromatographic Determination ◽  
Florisil Column ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract The gas-liquid chromatographic method for the determination of T-2 toxin in plasma is described. The toxin is extracted with benzene, washed with aqueous sodium hydroxide, and chromatographed on a small Florisil column; the heptafluorobutyryl derivative is prepared by reaction with heptafluorobutyrylimidazole. The T-2 HFB derivative is chromatographed onOV-1 at 230°C and measured with an electron capture detector. Iso-T-2, an isomer of T-2 toxin, is added to samples as an internal standard before extraction. Recoveries averaged 98.0 ± 5.5% at levels ranging from 50 to 1000 ng/m L. The limit of detection is 25 ng/mL.

Liquid Chromatographic Determination of Morphine Sulfate and Some Contaminants in Injections and Bulk Drug Material: Collaborative Study

1988 ◽  
Vol 71 (5) ◽  
pp. 1046-1048
Author(s):  
Ada C Bello ◽  
Rita K Jhangiani
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Morphine Sulfate ◽  
System Suitability ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Bulk Drug ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the assay of morphine sulfate and some preservatives and impurities in the bulk drug and in injections has been developed and collaboratively studied in 8 laboratories. Each collaborator analyzed 5 samples: 1 bulk drug, 3 different concentrations of injectable dosages, and 1 prepared mixture containing, in addition to morphine sulfate, phenol, 2-mercaptobenzothiazole, and pseudomorphine. The proposed method quantitates morphine sulfate and resolves the other components for identification using a Clg reverse-phase column with a mobile solvent containing 240 mL methanol, 720 mL 0.005M 1-heptanesulfonic acid Na salt, and 10 mL acetic acid. Samples are prepared by direct dilution with mobile solvent minus 1-heptanesulfonic acid. All collaborators met system suitability requirements and performed the analysis without difficulty. No outliers were found when data were analyzed by the Dixon, Grubbs, double Grubbs, and Cochran tests. Relative standard deviations between laboratories (RSDR) for duplicate determinations of morphine sulfate ranged from 1.4 to 2.1%. Mean morphine sulfate recoveries for the bulk drug and the prepared mixture were 100.8 and 100.4%, respectively. The method has been approved interim official first action.

Liquid Chromatographic Determination of Coumarin Anticoagulants in Tablets: Collaborative Study

1987 ◽  
Vol 70 (5) ◽  
pp. 834-836
Author(s):  
Ella S Moore
Keyword(s):  
Mobile Phase ◽  
Collaborative Study ◽  
Chromatographic Determination ◽  
Content Uniformity ◽  
Photometric Detection ◽  
Warfarin Sodium ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the determination of coumarin anticoagulants in tablets was collaboratively studied by 7 laboratories. The method uses an octadecylsilane-bonded microparticulate column, tetrahydrofuran-methanol-water-acetic acid mobile phase, and photometric detection at 311 nm. Each collaborator received samples of warfarin sodium, phenprocoumon, and dicumarol as a synthetic composite and as commercial individual and composited tablets. Pooled average assay values for synthetic and commercial tablet samples of warfarin sodium were 101.6 and 99.5%, respectively, with a combined reproducibility SD of 2.38% (CV = 2.37%) and combined repeatability SD of 1.49% (CV = 1.49%). Pooled average (SD) assay values for dicumarol and phenprocoumon commercial samples were 98.0 (2.27) and 101.3% (4.00), respectively. The content uniformity determinations of 2 mg warfarin sodium and 25 mg dicumarol tablets indicated average tablet contents (range) of 99.5% (91.0-116.0) and 98.0% (89.8-108.8), respectively. The method has been approved interim official first action

High Performance Liquid Chromatographic Determination of Primidone in Tablets

1982 ◽  
Vol 65 (5) ◽  
pp. 1063-1065
Author(s):  
Stanley E Roberts
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Average Recovery ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A high performance liquid chromatographic (HPLC) method is described for the quantitative determination of primidone in tablets. A ground tablet sample is diluted directly in the mobile phase, at a concentration of about 1 mg/mL of primidone, mixed and deaerated, and filtered. The resulting solution is then quantitated by HPLC. The average spike recoveries for the 50 mg and 250 mg tablets were 101.2% and 99.0%, respectively. The average recovery for an authentic mixture formulated at the 250 mg level was 100.1% with a relative standard deviation of 0.45%.

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