Liquid Chromatographic-Fluorometric Determination of Cinnamyl Alcohol in Perfumes, Colognes, and Toilet Waters

1988 ◽  
Vol 71 (4) ◽  
pp. 821-823
Author(s):  
Harris H Wisneski ◽  
Ronald L Yates ◽  
John A Wenninger
Keyword(s):  
Emission Intensity ◽  
Fluorescence Emission ◽  
Water Mixture ◽  
Cinnamyl Alcohol ◽  
Fluorometric Determination ◽  
Short Column ◽  
Fluorescence Emission Intensity ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method is described for the determination of cinnamyl alcohol (3-phenyl-2-propen-l-ol) in fragrance compositions. The fragrance product is partially cleaned up by diluting the fragrance with a 95% ethanol-water mixture and passing it through a short column containing RP-8 packing. An aliquot of the effluent is then analyzed by LC using an RP-18 column interfaced to a spectrophotofluorometer equipped with double monochromators. The fluorescence emission intensity of the eluted cinnamyl alcohol is measured and compared with that of a standard to calculate the amount of cinnamyl alcohol present. Recoveries from fragrance products fortified with cinnamyl alcohol at levels ranging from 0.0020 to 0.060 mg/mL ranged from 85 to 105% with a mean of 94%. The lowest level of determination was 0.0005 mg/mL.

Liquid Chromatographic Separation and Fluorometric Determination of cw- and frans-Isoeugenol in Perfumes, Colognes, and Toilet Waters

1988 ◽  
Vol 71 (4) ◽  
pp. 818-820
Author(s):  
Harris H Wisneski ◽  
Ronald L Yates ◽  
John A Wenninger
Keyword(s):  
Sodium Hydroxide Solution ◽  
Relative Concentration ◽  
Fluorescence Emission ◽  
Test Portion ◽  
Limit Of Determination ◽  
Liquid Chromatographic Separation ◽  
Fluorescence Emission Intensity ◽  
Cis And Trans ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC)-fluorometric method is described for the determination of cis- and frans-isoeugenol (2-methoxy-4-propenylphenol) in perfumes, colognes, and toilet waters. A test portion of the product is added to diethyl ether, and the isoeugenol isomers are extracted with sodium hydroxide solution. The basic extract is then acidified, and the isoeugenol isomers are extracted with isooctane. Aliquots of the isooctane extract are analyzed by using a silver ion cation exchange LC column interfaced to a spectrophotofluorometer. Each isomer in the product is determined by comparing its fluorescence emission intensity with that of an external standard consisting of a mixture of both isomers in which the relative concentration of each has been determined. Average recoveries from various commercial fragrances fortified with a mixture of cis- and trans-isoeugenol with total isoeugenol content of 0.1,0.5, and 4.0 mg/mL ranged from 87 to 105% for the taww-isomer (SD = 4.6%) and from 83 to 113% for the cw-isomer (SD = 6.7%). The limit of determination is approximately 0.002 mg/mL.

Temperature Dependence of the Fluorescence Emission Intensity of Eu/TiO 2 Nanocrystals

2007 ◽  
Vol 24 (5) ◽  
pp. 1368-1371 ◽  
Author(s):  
Zeng Qing-Guang ◽  
Ding Ze-Jun ◽  
Ju Xin ◽  
Wang Yi ◽  
Sheng Ye-Qing
Keyword(s):  
Temperature Dependence ◽  
Emission Intensity ◽  
Fluorescence Emission ◽  
Fluorescence Emission Intensity

Reply to the ‘Comment on “Simple fluorescence-based detection of Cr(iii) and Cr(vi) using unmodified gold nanoparticles”’ by M. R. Hormozi-Nezhad, J. Mohammadi and A. Bigdeli, Anal. Methods, 2015, 7, DOI: 10.1039/c5ay00005j

2015 ◽  
Vol 7 (14) ◽  
pp. 6035-6036
Author(s):  
Sruthi Ann Alex ◽  
M. Elavarasi ◽  
D. Nanda Kumar ◽  
A. Rajeshwari ◽  
N. Chandrasekaran ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Emission Intensity ◽  
Fluorescence Emission ◽  
Fluorescence Emission Intensity

AuNP aggregation by Cr(iii) causes the reduction of the fluorescence emission intensity at 582 nm (λex = 490 nm).

scholarly journals FLUORESCENCE CHARACTERISTICS OF FLUORESCEIN AND EOSIN Y SOLUTIONS IN WATER-MICELLAR SURFACTANT MEDIA

2020 ◽  
pp. 23-26
Author(s):  
V. Klovak ◽  
L. Nechpai ◽  
S. Lelyushok ◽  
S. Kulichenko
Keyword(s):  
Anionic Surfactant ◽  
Emission Intensity ◽  
Signal Intensity ◽  
Sodium Dodecyl ◽  
Fluorescence Emission ◽  
Ionic Surfactant ◽  
Eosin Y ◽  
Fluorescence Characteristics ◽  
Fluorescence Emission Intensity ◽  
Pharmacologically Active

The effect of cationic, anionic and nonionic surfactants on the fluorescence properties of fluorescein and eosin Y aqueous solutions has been investigated. It has been found that sodium dodecyl sulfate does not affect the position of the maximum wavelengths of solutions of fluorescein and eosin Y in the study of the effect of an anionic surfactant on the fluorescence emission intensity of dyes. The intensity of the signal of the dye solutions when changing the concentration of anionic surfactant changes little. As the concentration of non-ionic surfactant increases, the fluorescence emission intensity of the fluorescein solutions decreases. In contrast, with increasing concentration of Triton X-100, there is an increase in the signal intensity of solutions of more hydrophobic eosin Y with subsequent access to the "plateau" at СТХ-100≥5.1·10–2 mol/L. The position of the maxima fluorescence emission wavelengths for the fluorescein solutions in the 0-1.0·10–5 mol/L range of concentrations of cationic surfactant cetylpyridinium chloride remain unchanged. The position of the maxima shifts to the long-wavelength region of the spectrum at higher concentrations of CPC. The nature of the position dependence of the fluorescence emission maxima of eosin Y solutions in the presence of cationic surfactants is similar. The effect of fluorescence quenching has also been shown in the study of the influence of organic substances of cationic nature on the signal intensity of fluorescein solutions. It has been shown that the concentration dependence is linear in the (0.1–4.0)·10–1 mol/L range of isoniazid molecule concentrations. The data obtained can be implemented in the development of conditions and methods for the determination of pharmacologically active substances of cationic nature by reaction with fluorescein in medicines.

A study of fluorescence emission intensity of reaction products between selected amino acdis and DFO, 1,2-indanedione and 1,2-indanedione - zinc chloride

2014 ◽  
Vol 285 ◽  
pp. 68-72
Author(s):  
Tomasz Szczepański ◽  
◽  
Urszula Więckiewicz ◽  
Krzysztof Klemczak ◽  
Anna Chyczewska ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fluorescence Intensity ◽  
Zinc Chloride ◽  
Emission Intensity ◽  
Storage Time ◽  
Spectral Characteristics ◽  
Fluorescence Emission ◽  
Reaction Products ◽  
Intensity Measurements ◽  
Fluorescence Emission Intensity

The study shows the results of fluorescence emission intensity measurements of the reaction products of selected amino acids with DFO, 1,2-IND and 1,2-IND - zinc chloride on absorptive surfaces. Conducted research addressed the following variables: type of developing reagent, type of surface, type of amino acid and sample storage time. It was confirmed that the above factors affected the fluorescence intensity of developed fingerprints. Furthermore, the studies proved that fluorescence intensity of reaction products between amino acids and 1,2- IND stored for 1 or 7 days increased for the majority of samples upon addition of zinc chloride to the developing reagent. For samples stored for 4 months the highest fluorescence emission intensity was observed for DFO. No significant differences were found in fluorescence spectral characteristics of tested compounds, depending on the type of surface.

Spectroscopic and Fluorometric Characterization of Oxacyanine Dyes in Water and Nitrobenzene

1988 ◽  
Vol 42 (6) ◽  
pp. 958-961 ◽  
Author(s):  
Deborah H. Wiegand ◽  
Petr Vanýsek
Keyword(s):  
Emission Intensity ◽  
Partition Coefficients ◽  
Fluorescence Emission ◽  
Red Shift ◽  
Electrochemical Studies ◽  
Interface System ◽  
Fluorescence Emission Intensity ◽  
20 Nm

The spectroscopic and fluorometric properties of nine oxacyanine dyes (3,3′-dimethyloxacarbocyanine through 3,3′-diheptyloxacarbocyanine, 3,3′-diethyl-, and 3,3′-dipropyloxadicarbocyanine iodides) were determined in two solvents, water and nitrobenzene. In nitrobenzene the absorbance and fluorometric spectra of the dyes showed a red shift of 15 to 20 nm when compared with the spectra in water. In addition, the fluorescence emission intensity of the dyes is lower in nitrobenzene than in water, by one to two orders of magnitude. Thus it is possible to measure fluorescence in water in water/nitrobenzene systems without interference from the nitrobenzene part. The molar absorptivities of the dyes were calculated and used to determine the solubility of the dyes in water and the partition coefficients of the dyes between nitrobenzene and water. These dyes were characterized in order to provide a better understanding of the properties of the dyes in a water/nitrobenzene interface system which will be used in electrochemical studies of the dyes.

Fluorometric Determination of Indole in Shrimp

1978 ◽  
Vol 61 (5) ◽  
pp. 1089-1091
Author(s):  
Carl Ponder
Keyword(s):  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Analytical Data ◽  
Methanol Solution ◽  
Fluorometric Method ◽  
Fluorometric Determination ◽  
Saturated Sodium Chloride Solution ◽  
Aoac Official ◽  
Liquid Chromatographic

Abstract A fluorometric method is described for determining indole in shrimp. The indole is extracted with n-hexane, partitioned into a methanol- saturated sodium chloride solution ( 9 + 1 ), and determined fluoromctrically. The detection limit of the method is 0.04 μg indole/g shrimp. Quantitative analytical data are presented for indole in samples of shrimp determined by the fluorometric method and the AOAC official colorimetric and gas-liquid chromatographic procedures. The correlation coefficient between the data of the fluorometric and colorimetric methods was 0.96. Indole recovered from 25 g samples of fresh shrimp spiked with 6 and 12 μg indole/25 g ranged from 97 to 106%. The fluorescence response is linear in the range of 1 to 25 μg indole/100 ml methanol solution and no significant change is noted in solutions kept in the dark 18 hr.

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