scholarly journals Evaluation of Commercial Chlorophyllin Copper Complex Preparations by Liquid Chromatography with Photodiode Array Detection

1997 ◽  
Vol 80 (2) ◽  
pp. 433-435 ◽  
Author(s):  
Simon Chernomorsky ◽  
Raymond Rancourt ◽  
Divya Sahai ◽  
Ronald Poretz
Keyword(s):  
Copper Complex ◽  
Copper Complexes ◽  
Reversed Phase ◽  
Pheophorbide A ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection ◽  
Phase Liquid ◽  
Industrial Sources ◽  
Liquid Chromatographic

Abstract A reversed-phase liquid chromatographic (LC) system with photodiode array detection was used to analyze chlorophyllin copper complex (CCC). Analysis revealed significant differences in the porphyrin compositions of CCC samples from 5 industrial sources. Copper isochlorin e4 was identified as a major component in most commercial materials. Copper complexes of chlorin e6 and pheophorbide a and unidentified porphyrins with either chlorin- or non-chlorin-type spectra were found in some samples. The variability of porphyrin compositions in commercial preparations may affect the medicinal efficacy of CCC; therefore, analysis of porphyrin composition is important for CCC quality control.

Determination of clozapine, norclozapine, and clozapine-N-oxide in serum by liquid chromatography

1993 ◽  
Vol 39 (8) ◽  
pp. 1656-1659 ◽  
Author(s):  
S A Volpicelli ◽  
F Centorrino ◽  
P R Puopolo ◽  
J Kando ◽  
F R Frankenburg ◽  
...  
Keyword(s):  
Internal Standard ◽  
Reversed Phase ◽  
Therapeutic Monitoring ◽  
Pharmacokinetic Studies ◽  
Photodiode Array Detection ◽  
Photodiode Array ◽  
Array Detection ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract We report a new assay to measure the serum concentrations of the atypical antipsychotic drug clozapine and two major metabolites, norclozapine and clozapine-N-oxide. The analytes and an internal standard (triprolidine) were extracted from alkalinized samples into ethyl acetate and back-extracted into 0.1 mol/L HCl. The acid extracts were chromatographed on a reversed-phase liquid chromatographic column with photodiode array detection (210-340 nm). With the 254-nm signal, between-run imprecision (CV) was < 2% for clozapine and norclozapine at 400 micrograms/L, and 4.1% for clozapine-N-oxide at 100 micrograms/L. Absolute recovery exceeded 65%, and the detection limit was approximately 3-4 micrograms/L. In 25 patients at steady state at a mean daily clozapine dosage of 269 mg (3.09 mg/kg), clozapine averaged 231 +/- 144 micrograms/L (mean +/- SD); norclozapine and clozapine-N-oxide concentrations averaged 84% and 23% that of clozapine. Analyte concentrations were significantly correlated with daily dose. The method's ability to quantify clozapine and two major metabolites simultaneously with precision and sensitivity makes it useful in pharmacokinetic studies and therapeutic monitoring.

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