scholarly journals Assessment of microchimerism following somatic cell nuclear transfer and natural pregnancies in goats

2019 ◽  
Vol 97 (9) ◽  
pp. 3786-3794 ◽  
Author(s):  
Kirsten K Gash ◽  
Min Yang ◽  
Zhiqiang Fan ◽  
Misha Regouski ◽  
Heloisa M Rutigliano ◽  
...  
Keyword(s):  
Lymph Node ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Small Population ◽  
Cell Trafficking ◽  
Maternal Cell ◽  
Fetal Microchimerism ◽  
Natural Breeding ◽  
Dna Trafficking

AbstractMicrochimerism is defined as the presence of a small population of cells or DNA in 1 organism originated from a genetically different organism. It is well established that this phenomenon occurs in humans and mice as cells are exchanged between mother and fetus during gestation. Currently, no information is available about the presence of maternal microchimerism in goats, and the only published study is limited to an evaluation of fetal and fetal–fetal microchimerism in blood samples following natural breeding. In order to determine whether bidirectional fetal–maternal cell or DNA trafficking occurs in goats, we assessed: 1) fetal microchimerism in surrogates that gave birth to somatic cell nuclear transfer (SCNT)-derived transgenic offspring (n = 4), 2) maternal microchimerism following natural breeding of SCNT-derived transgenic does with a nontransgenic buck (n = 4), and 3) fetal–fetal microchimerism in nontransgenic twins of transgenic offspring (n = 3). Neomycin-resistance gene (NEO) gene was selected as the marker to detect the presence of the αMHC-TGF-β1-Neo transgene in kidney, liver, lung, lymph node, and spleen. We found no detectable maternal or fetal–fetal microchimerism in the investigated tissues of nontransgenic offspring. However, fetal microchimerism was detected in lymph node tissue of one of the surrogate dams carrying a SCNT pregnancy. These results indicate occurrence of cell trafficking from fetus to mother during SCNT pregnancies. The findings of this study have direct implications on the use and disposal of nontransgenic surrogates and nontransgenic offspring.

30 GROWTH AND REPRODUCTIVE SUCCESS OF CLONED MALE CATS DERIVED FROM ADULT SOMATIC CELL NUCLEAR TRANSFER

2007 ◽  
Vol 19 (1) ◽  
pp. 133
Author(s):  
E. G. Choi ◽  
X. J. Yin ◽  
H. S. Lee ◽  
S. H. Kang ◽  
Y. J. Lee ◽  
...  
Keyword(s):  
Reproductive Success ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Normal Growth ◽  
Gonadal Hormones ◽  
The Other ◽  
Eye Color ◽  
Natural Breeding ◽  
Natural Mating

There have been very few published reports on the normal growth and fertility of cloned cats derived from adult somatic cell nuclear transfer (SCNT). Here, we report studies investigating the growth and fertility of cloned male cats produced by SCNT. This study was designed to investigate the reproductive success by natural breeding of cloned male cats with domestic female cats and to measure endocrine hormone profiles related to male reproduction such as testosterone, LH, and FSH. Clones A, B, and C produced 3, 2, and 4 kittens, respectively, after natural mating with 4 domestic female cats, and clone D produced 5 kittens, in spite of being further into puberty than the other cloned male cats. All of the females delivered at 64 to 66 days after natural mating. The results obtained are presented as the mean � SEM. Sexual hormones were analyzed by ANOVA. All statistical analysis was conducted with SAS (SAS Institute Inc., Cary, NC, USA), and P < 0.05 was considered significant. Three of the 14 kittens expressed an odd eye color, which was produced from 1 clone A and 2 clone B cats. The eye color of other F1 kittens varied from brown to blue. Body weight at birth ranged from 72.9 to 134 g. Although clone D had a lower libido and entered puberty later than the other cloned males, it produced gonadal hormones within the average range. Four cloned male cats had normal fertility and were free from any reproductive problems. All of the cloned male cats were similar to two control and donor cats with respect to concentration of gonadal hormones. However, the concentration of testosterone in a castrated donor cat was significantly lower than that from clones A, B, C, D, and control cats (0.4 � 0.1 vs. 5.99 � 5.68; 3.46 � 2.81; 6.41 � 2.17; 3.75 � 0.34; 4.0 � 3.63 ng mL-1; P < 0.05). The concentrations of LH and FSH were not significantly different among any of the cats. A total of 14 kittens, 7 male and 7 female, were produced by the natural breeding of cloned male cats with 4 domestic female cats. These results indicate that cloned male cats have normal reproductive success and lie within the normal range of gonadal hormone production. All of the F1 kittens were produced by natural breeding, delivery, demonstrated normal growth, and are still alive and healthy. This work was supported by KOSEF (grant M10525010001-05N2501-00110).

scholarly journals Author Correction: Application of interspecific Somatic Cell Nuclear Transfer (iSCNT) in sturgeons and an unexpectedly produced gynogenetic sterlet with homozygous quadruple haploid

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Effrosyni Fatira ◽  
Miloš Havelka ◽  
Catherine Labbé ◽  
Alexandra Depincé ◽  
Viktoriia Iegorova ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer

scholarly journals Stem cell therapies and benefaction of somatic cell nuclear transfer cloning in COVID-19 era

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Birbal Singh ◽  
Gorakh Mal ◽  
Vinod Verma ◽  
Ruchi Tiwari ◽  
Muhammad Imran Khan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Drug Testing ◽  
Human Interaction ◽  
Patient Specific ◽  
Wide Range

Abstract Background The global health emergency of COVID-19 has necessitated the development of multiple therapeutic modalities including vaccinations, antivirals, anti-inflammatory, and cytoimmunotherapies, etc. COVID-19 patients suffer from damage to various organs and vascular structures, so they present multiple health crises. Mesenchymal stem cells (MSCs) are of interest to treat acute respiratory distress syndrome (ARDS) caused by SARS-CoV-2 infection. Main body Stem cell-based therapies have been verified for prospective benefits in copious preclinical and clinical studies. MSCs confer potential benefits to develop various cell types and organoids for studying virus-human interaction, drug testing, regenerative medicine, and immunomodulatory effects in COVID-19 patients. Apart from paving the ways to augment stem cell research and therapies, somatic cell nuclear transfer (SCNT) holds unique ability for a wide range of health applications such as patient-specific or isogenic cells for regenerative medicine and breeding transgenic animals for biomedical applications. Being a potent cell genome-reprogramming tool, the SCNT has increased prominence of recombinant therapeutics and cellular medicine in the current era of COVID-19. As SCNT is used to generate patient-specific stem cells, it avoids dependence on embryos to obtain stem cells. Conclusions The nuclear transfer cloning, being an ideal tool to generate cloned embryos, and the embryonic stem cells will boost drug testing and cellular medicine in COVID-19.

Melatonin significantly improves the developmental competence of bovine somatic cell nuclear transfer embryos

2015 ◽  
Vol 59 (4) ◽  
pp. 455-468 ◽  
Author(s):  
Jianmin Su ◽  
Yongsheng Wang ◽  
Xupeng Xing ◽  
Lei Zhang ◽  
Hongzheng Sun ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Developmental Competence
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