30 GROWTH AND REPRODUCTIVE SUCCESS OF CLONED MALE CATS DERIVED FROM ADULT SOMATIC CELL NUCLEAR TRANSFER

2007 ◽  
Vol 19 (1) ◽  
pp. 133
Author(s):  
E. G. Choi ◽  
X. J. Yin ◽  
H. S. Lee ◽  
S. H. Kang ◽  
Y. J. Lee ◽  
...  
Keyword(s):  
Reproductive Success ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Normal Growth ◽  
Gonadal Hormones ◽  
The Other ◽  
Eye Color ◽  
Natural Breeding ◽  
Natural Mating

There have been very few published reports on the normal growth and fertility of cloned cats derived from adult somatic cell nuclear transfer (SCNT). Here, we report studies investigating the growth and fertility of cloned male cats produced by SCNT. This study was designed to investigate the reproductive success by natural breeding of cloned male cats with domestic female cats and to measure endocrine hormone profiles related to male reproduction such as testosterone, LH, and FSH. Clones A, B, and C produced 3, 2, and 4 kittens, respectively, after natural mating with 4 domestic female cats, and clone D produced 5 kittens, in spite of being further into puberty than the other cloned male cats. All of the females delivered at 64 to 66 days after natural mating. The results obtained are presented as the mean � SEM. Sexual hormones were analyzed by ANOVA. All statistical analysis was conducted with SAS (SAS Institute Inc., Cary, NC, USA), and P < 0.05 was considered significant. Three of the 14 kittens expressed an odd eye color, which was produced from 1 clone A and 2 clone B cats. The eye color of other F1 kittens varied from brown to blue. Body weight at birth ranged from 72.9 to 134 g. Although clone D had a lower libido and entered puberty later than the other cloned males, it produced gonadal hormones within the average range. Four cloned male cats had normal fertility and were free from any reproductive problems. All of the cloned male cats were similar to two control and donor cats with respect to concentration of gonadal hormones. However, the concentration of testosterone in a castrated donor cat was significantly lower than that from clones A, B, C, D, and control cats (0.4 � 0.1 vs. 5.99 � 5.68; 3.46 � 2.81; 6.41 � 2.17; 3.75 � 0.34; 4.0 � 3.63 ng mL-1; P < 0.05). The concentrations of LH and FSH were not significantly different among any of the cats. A total of 14 kittens, 7 male and 7 female, were produced by the natural breeding of cloned male cats with 4 domestic female cats. These results indicate that cloned male cats have normal reproductive success and lie within the normal range of gonadal hormone production. All of the F1 kittens were produced by natural breeding, delivery, demonstrated normal growth, and are still alive and healthy. This work was supported by KOSEF (grant M10525010001-05N2501-00110).

scholarly journals Assessment of microchimerism following somatic cell nuclear transfer and natural pregnancies in goats

2019 ◽  
Vol 97 (9) ◽  
pp. 3786-3794 ◽  
Author(s):  
Kirsten K Gash ◽  
Min Yang ◽  
Zhiqiang Fan ◽  
Misha Regouski ◽  
Heloisa M Rutigliano ◽  
...  
Keyword(s):  
Lymph Node ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Small Population ◽  
Cell Trafficking ◽  
Maternal Cell ◽  
Fetal Microchimerism ◽  
Natural Breeding ◽  
Dna Trafficking

AbstractMicrochimerism is defined as the presence of a small population of cells or DNA in 1 organism originated from a genetically different organism. It is well established that this phenomenon occurs in humans and mice as cells are exchanged between mother and fetus during gestation. Currently, no information is available about the presence of maternal microchimerism in goats, and the only published study is limited to an evaluation of fetal and fetal–fetal microchimerism in blood samples following natural breeding. In order to determine whether bidirectional fetal–maternal cell or DNA trafficking occurs in goats, we assessed: 1) fetal microchimerism in surrogates that gave birth to somatic cell nuclear transfer (SCNT)-derived transgenic offspring (n = 4), 2) maternal microchimerism following natural breeding of SCNT-derived transgenic does with a nontransgenic buck (n = 4), and 3) fetal–fetal microchimerism in nontransgenic twins of transgenic offspring (n = 3). Neomycin-resistance gene (NEO) gene was selected as the marker to detect the presence of the αMHC-TGF-β1-Neo transgene in kidney, liver, lung, lymph node, and spleen. We found no detectable maternal or fetal–fetal microchimerism in the investigated tissues of nontransgenic offspring. However, fetal microchimerism was detected in lymph node tissue of one of the surrogate dams carrying a SCNT pregnancy. These results indicate occurrence of cell trafficking from fetus to mother during SCNT pregnancies. The findings of this study have direct implications on the use and disposal of nontransgenic surrogates and nontransgenic offspring.

scholarly journals 34EFFECT OF GENOTYPE AND CELL LINE ON THE EFFICIENCY OF LIVE CALF PRODUCTION BY SOMATIC CELL NUCLEAR TRANSFER

2004 ◽  
Vol 16 (2) ◽  
pp. 139 ◽  
Author(s):  
K. DeLegge ◽  
M. Maserati ◽  
N. Kieser ◽  
D. Delanski ◽  
B. Henderson ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Early Adulthood ◽  
The Other ◽  
Culture Techniques ◽  
Clostridial Infection ◽  
Healthy Calf

The efficiency of production of live calves using somatic cell nuclear transfer was compared among 52 different cell lines representing 43 different genotypes. Cell lines were not genetically modified. Nuclear transfer was performed according to methods described by Cibelli et al., 1998 Science 280, 1256–1258, with modifications. All cells were derived from either explant cultures or enzyme digests of skin biopsies and were cyropreserved and thawed at least 48 hours prior to nuclear transfer. Cells were harvested using either pronase or trypsin at 70 to 90% confluence. Oocytes were either activated prior to fusion or immediately after fusion using ionomycin. The couplets were then cultured in cycloheximide and cytochalsin B for 6 hours. In 36 cases (84%), at least one healthy calf was produced from the initial trial which included transfer to 10 to 20 recipients for each cell line. For 4 of the 7 cases where the initial cell line failed to produce a live calf, a new cell line was derived and the process repeated. In one case where the data are available from the second cell line, 5 live calves were produced from 20 recipients receiving embryos (25%). Results from the other repeated cell lines are pending. For 5 of the different genotypes, nuclear transfer was done at about the same time using two different cell lines, and 4 of these have produced healthy calves from both cell lines. In one case, one cell line produced live calves, and no calves were produced from the other cell line. In total, 167 calves were born, of which 107 are alive and healthy as of this writing (64%), and range in age from 1 to 25 months. There are 86 calves older than 6 months of age and no losses have occurred as calves have aged into early adulthood. Forty-four (26%) of the calves were stillborn, failed to convert to neonatal circulation or were euthanized within 48 hours of birth. The most frequent reason for euthanasia was severe contracture of the limbs (arthrogryposis). This defect occurred even within cell lines that also gave rise to healthy calves, although it was more prevalent with certain cell lines. Other complications among the normal calves born were those of an abnormally large umbilicus or umbilical vessels. In addition, 16 calves were lost after the first 48 hours (13%). Two of these losses were due to accidents and 9 of them were due to complications from umbilical infections. The other 5 calf loses resulted from complications common to young calves such as clostridial infection and ruptured abomasum. Recent improvements in cell line derivation and embryo culture techniques, as well as a higher incidence of natural birth and improved neonatal management, have resulted in healthy calf production efficiencies (from embryos transferred) greater than 30% for 5 independent genotypes. The number of healthy calves produced per embryo transferred was 11 of 20 (55%), 5 of 10 (50%), 5 of 10 (50%), 4 of 11 (36%), and 3 of 10 (30%), for each of these genotypes, respectively. There was no correlation between the efficiency of blastocyst production and pregnancy outcome for the cell lines evaluated in this study. In conclusion, the efficiency of live healthy calf production using somatic cell nuclear transfer remains variable, depending on both the cell line and the genotype. However, efficiencies approaching those obtained using conventional embryo transfer is possible.

scholarly journals Author Correction: Application of interspecific Somatic Cell Nuclear Transfer (iSCNT) in sturgeons and an unexpectedly produced gynogenetic sterlet with homozygous quadruple haploid

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Effrosyni Fatira ◽  
Miloš Havelka ◽  
Catherine Labbé ◽  
Alexandra Depincé ◽  
Viktoriia Iegorova ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer

scholarly journals Stem cell therapies and benefaction of somatic cell nuclear transfer cloning in COVID-19 era

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Birbal Singh ◽  
Gorakh Mal ◽  
Vinod Verma ◽  
Ruchi Tiwari ◽  
Muhammad Imran Khan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Drug Testing ◽  
Human Interaction ◽  
Patient Specific ◽  
Wide Range

Abstract Background The global health emergency of COVID-19 has necessitated the development of multiple therapeutic modalities including vaccinations, antivirals, anti-inflammatory, and cytoimmunotherapies, etc. COVID-19 patients suffer from damage to various organs and vascular structures, so they present multiple health crises. Mesenchymal stem cells (MSCs) are of interest to treat acute respiratory distress syndrome (ARDS) caused by SARS-CoV-2 infection. Main body Stem cell-based therapies have been verified for prospective benefits in copious preclinical and clinical studies. MSCs confer potential benefits to develop various cell types and organoids for studying virus-human interaction, drug testing, regenerative medicine, and immunomodulatory effects in COVID-19 patients. Apart from paving the ways to augment stem cell research and therapies, somatic cell nuclear transfer (SCNT) holds unique ability for a wide range of health applications such as patient-specific or isogenic cells for regenerative medicine and breeding transgenic animals for biomedical applications. Being a potent cell genome-reprogramming tool, the SCNT has increased prominence of recombinant therapeutics and cellular medicine in the current era of COVID-19. As SCNT is used to generate patient-specific stem cells, it avoids dependence on embryos to obtain stem cells. Conclusions The nuclear transfer cloning, being an ideal tool to generate cloned embryos, and the embryonic stem cells will boost drug testing and cellular medicine in COVID-19.

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