scholarly journals Retrospective Detection of Sulfur Mustard Exposure by Mass Spectrometric Analysis of Adducts to Albumin and Hemoglobin: An In Vivo Study

2008 ◽  
Vol 32 (1) ◽  
pp. 25-30 ◽  
Author(s):  
D. Noort ◽  
A. Fidder ◽  
C.E.A.M. Degenhardt-Langelaan ◽  
A.G. Hulst
Keyword(s):  
Sulfur Mustard ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
In Vivo Study ◽  
Spectrometric Analysis

scholarly journals TDP-l-Megosamine Biosynthesis Pathway Elucidation and Megalomicin A Production in Escherichia coli

2010 ◽  
Vol 76 (12) ◽  
pp. 3869-3877 ◽  
Author(s):  
Mariana Useglio ◽  
Salvador Peirú ◽  
Eduardo Rodríguez ◽  
Guillermo R. Labadie ◽  
John R. Carney ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Biosynthesis Pathway ◽  
Biosynthetic Genes

ABSTRACT In vivo reconstitution of the TDP-l-megosamine pathway from the megalomicin gene cluster of Micromonospora megalomicea was accomplished by the heterologous expression of its biosynthetic genes in Escherichia coli. Mass spectrometric analysis of the TDP-sugar intermediates produced from operons containing different sets of genes showed that the production of TDP-l-megosamine from TDP-4-keto-6-deoxy-d-glucose requires only five biosynthetic steps, catalyzed by MegBVI, MegDII, MegDIII, MegDIV, and MegDV. Bioconversion studies demonstrated that the sugar transferase MegDI, along with the helper protein MegDVI, catalyzes the transfer of l-megosamine to either erythromycin C or erythromycin D, suggesting two possible routes for the production of megalomicin A. Analysis in vivo of the hydroxylation step by MegK indicated that erythromycin C is the intermediate of megalomicin A biosynthesis.

scholarly journals Use of Quantitative Mass Spectrometric Analysis to Elucidate the Mechanisms of Phospho-priming and Auto-activation of the Checkpoint Kinase Rad53 in Vivo

2013 ◽  
Vol 13 (2) ◽  
pp. 551-565 ◽  
Author(s):  
Eric S.-W. Chen ◽  
Nicolas C. Hoch ◽  
Shun-Chang Wang ◽  
Achille Pellicioli ◽  
Jörg Heierhorst ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Checkpoint Kinase

scholarly journals Gas Chromatographic-Mass Spectrometric Analysis of Sulfur Mustard-Plasma Protein Adducts: Validation and Use in a Rat Inhalation Model*

2008 ◽  
Vol 32 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Benedict R. Capacio ◽  
J. Richard Smith ◽  
Richard J. Lawrence ◽  
Brian L. Boyd ◽  
Alicia M. Witriol ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Sulfur Mustard ◽  
Mass Spectrometric ◽  
Protein Adducts ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Chromatographic Mass
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