scholarly journals Use of Quantitative Mass Spectrometric Analysis to Elucidate the Mechanisms of Phospho-priming and Auto-activation of the Checkpoint Kinase Rad53 in Vivo

2013 ◽  
Vol 13 (2) ◽  
pp. 551-565 ◽  
Author(s):  
Eric S.-W. Chen ◽  
Nicolas C. Hoch ◽  
Shun-Chang Wang ◽  
Achille Pellicioli ◽  
Jörg Heierhorst ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Checkpoint Kinase

scholarly journals TDP-l-Megosamine Biosynthesis Pathway Elucidation and Megalomicin A Production in Escherichia coli

2010 ◽  
Vol 76 (12) ◽  
pp. 3869-3877 ◽  
Author(s):  
Mariana Useglio ◽  
Salvador Peirú ◽  
Eduardo Rodríguez ◽  
Guillermo R. Labadie ◽  
John R. Carney ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Biosynthesis Pathway ◽  
Biosynthetic Genes

ABSTRACT In vivo reconstitution of the TDP-l-megosamine pathway from the megalomicin gene cluster of Micromonospora megalomicea was accomplished by the heterologous expression of its biosynthetic genes in Escherichia coli. Mass spectrometric analysis of the TDP-sugar intermediates produced from operons containing different sets of genes showed that the production of TDP-l-megosamine from TDP-4-keto-6-deoxy-d-glucose requires only five biosynthetic steps, catalyzed by MegBVI, MegDII, MegDIII, MegDIV, and MegDV. Bioconversion studies demonstrated that the sugar transferase MegDI, along with the helper protein MegDVI, catalyzes the transfer of l-megosamine to either erythromycin C or erythromycin D, suggesting two possible routes for the production of megalomicin A. Analysis in vivo of the hydroxylation step by MegK indicated that erythromycin C is the intermediate of megalomicin A biosynthesis.

scholarly journals In vivo investigation of female reproductive functions and parameters in nonpregnant mice models and mass spectrometric analysis of the methanol leaf extract ofEmilia Coccinea(Sims) G Dons

2016 ◽  
Vol 4 (23) ◽  
pp. e13047 ◽  
Author(s):  
Uloma B. Elvis-Offiah ◽  
Enitome E. Bafor ◽  
Gerald I. Eze ◽  
Osamwonyi Igbinumwen ◽  
Christina Viegelmann ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis

The composition of prostate core matrisome in vivo and in vitro unveiled by mass spectrometric analysis

The Prostate ◽  
2018 ◽  
Vol 78 (8) ◽  
pp. 583-594 ◽  
Author(s):  
Marjaana Ojalill ◽  
Pekka Rappu ◽  
Elina Siljamäki ◽  
Pekka Taimen ◽  
Peter Boström ◽  
...  
Keyword(s):  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis

A Questionnaire Survey on General Status and Opinions about Clinical Mass Spectrometric Analysis in Korea (2018)

2019 ◽  
Vol 9 (3) ◽  
pp. 161
Author(s):  
Sung-Eun Cho ◽  
Hyojin Chae ◽  
Hyung-Doo Park ◽  
Sail Chun ◽  
Yong-Wha Lee ◽  
...  
Keyword(s):  
Questionnaire Survey ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
General Status

Mechanisms of Dopant Depth Profile Modification During Mass Spectrometric Analysis of Multilayer Structure

2015 ◽  
Vol 60 (6) ◽  
pp. 511-520 ◽  
Author(s):  
A.A. Efremov ◽  
◽  
V.G. Litovchenko ◽  
V.P. Melnik ◽  
O.S. Oberemok ◽  
...  
Keyword(s):  
Depth Profile ◽  
Multilayer Structure ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Profile Modification
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