Insecticidal and Behavioral Effect of Microparticles of Pimpinella anisum Essential Oil on Larvae of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Abstract The Colorado potato beetle ranks as one of the most important potato pests, mainly due to its high feeding rate during all developmental stages, particularly third and fourth larval instar, and high fecundity. The effect of essential oil (EO) from anise (Pimpinella anisum L. [Apiales: Apiaceae]) prepared as conventional and encapsulated (EN) formulations on the mortality and antifeedant responses of young larvae of Colorado potato beetles was studied to evaluate the insecticidal and antifeedant effects of five concentrations of this EO and to assess the persistence of both formulations on potato plants. The EN formulation had a significantly higher residual amount compared with that of the conventionally formulated EO. Significantly different values of LC50 and LC90 (ppm) were established for the EO (LC50 = 1,700 and LC90 = 9500) and EN (LC50 = 3,100 and LC90 = 14,300) formulations. The effects of both P. anisum formulations (EO and EN) applied topically to Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) larvae were distinctly different from those observed with the contact treatment. At the highest concentration of 20,000 ppm, the mortality of the second instars of the L. decemlineata larvae did not exceed 25%. On the other hand, both tested formulations of P. anisum were highly effective when administered orally. The encapsulated EO formulation achieved a distinctly higher biological activity. Our results confirm that the EO from P. anisum, especially the encapsulated formulation, has high insecticidal properties that may lead to the development of new organic products for the control of Colorado potato beetles.

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THE INFLUENCE OF TANACETUM PARTHENIUM ESSENTIAL OIL AND THEIR CONSTITUENTS ON COLORADO POTATO BEETLE, LEPTINOTARSA DECEMLINEATA SAY

Acta Horticulturae ◽

10.17660/actahortic.1993.344.65 ◽

1993 ◽

pp. 565-570 ◽

Cited By ~ 2

Author(s):

M. Kostich ◽

M. Ristich ◽

A. Zabel ◽

V. Sekeschan ◽

O. Gaschich

Keyword(s):

Essential Oil ◽

Colorado Potato Beetle ◽

Leptinotarsa Decemlineata ◽

Tanacetum Parthenium ◽

Potato Beetle ◽

Leptinotarsa Decemlineata Say

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A Day in the Life: Identification of Developmentally Regulated MicroRNAs in the Colorado Potato Beetle (Leptinotarsa decemlineata; Coleoptera: Chrysomelidae)

Journal of Economic Entomology ◽

10.1093/jee/toaa020 ◽

2020 ◽

Vol 113 (3) ◽

pp. 1445-1454

Author(s):

K F Wiebe ◽

O O Elebute ◽

C M R LeMoine ◽

B J Cassone

Keyword(s):

Colorado Potato Beetle ◽

Leptinotarsa Decemlineata ◽

Developmental Stages ◽

Early Stage ◽

Control Strategies ◽

Solanum Tuberosum L ◽

Potato Beetle ◽

Important Pest ◽

Leptinotarsa Decemlineata Say ◽

Potential Applications

Abstract The Colorado potato beetle (Leptinotarsa decemlineata (Say)) is an important pest of the cultivated potato (Solanum tuberosum (L.) [Solanales: Solanaceae]). With its broad resistance toward commonly used insecticides, it is clear that more sophisticated control strategies are needed. Due to their importance in insect development, microRNAs (miRNAs) represent a potential tool to employ in insect control strategies. However, most studies conducted in this area have focused on model species with well-annotated genomes. In this study, next-generation sequencing was used to catalogue the miRNAs produced by L. decemlineata across all eight stages of its development, from eggs to adults. For most stages, the length of miRNAs peaked between 21 and 22 nt, though it was considerably longer for the egg stage (26 nt). Global profiling of miRNAs revealed three distinct developmental clusters: 1) egg stage; 2) early stage (first, second, and third instar); and 3) late stage (fourth instar, prepupae, pupae, and adult). We identified 86 conserved miRNAs and 33 bonafide novel miRNAs, including stage-specific miRNAs and those not previously identified in L. decemlineata. Most of the conserved miRNAs were found in multiple developmental stages, whereas the novel miRNAs were often stage specific with the bulk identified in the egg stage. The identified miRNAs have a myriad of putative functions, including growth, reproduction, and insecticide resistance. We discuss the putative roles of some of the most notable miRNAs in the regulation of L. decemlineata development, as well as the potential applications of this research in Colorado potato beetle management.

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Antifeedant activity and toxicity of some plant essential oils to Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae)

Plant Protection Science ◽

10.17221/9/2014-pps ◽

2014 ◽

Vol 50 (No. 4) ◽

pp. 207-216 ◽

Cited By ~ 10

Author(s):

A. Taghizadeh Saroukolai ◽

G. Nouri-Ganbalani ◽

J. Hadian ◽

H. Rafiee-Dastjerdi

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Leptinotarsa Decemlineata ◽

Mentha Spicata ◽

Relative Growth ◽

Plant Essential Oils ◽

Potential Control ◽

Potato Beetle ◽

Leptinotarsa Decemlineata Say ◽

Food And Feeding

Essential oils of Satureja khuzistanica Jamzad, Ocimum basilicum L., Myrtus communis L., Thymus daenensis Celak, Mentha spicata L., and Eugenia caryophyllus (Sprengel) were evaluated for nutritional indices and mortality of the 4th instar larvae and adults of Leptinotarsa decemlineata (Say). Relative growth rate, relative consumption rate, efficiency of conversion of ingested food and feeding deterrent index were measured. Results showed that the most efficient essential oil on the 4th instar larvae and adults was S. khuzistanica (LC50 = 23.36 and 167.96 ppm, respectively). Even if all essential oils were effective on feeding deterrence of both stages of L. decemlineata, the essential oil of S. khuzistanica was the most effective. So, these essential oils can be used as potential control agents against both stages of L. decemlineata.

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Contact Toxicities of Oxygenated Monoterpenes to Different Populations of Colorado Potato Beetle, Leptinotarsa Decemlineata Say (Coleoptera: Chrysomelidae)

Journal of Plant Protection Research ◽

10.2478/v10045-011-0038-4 ◽

2011 ◽

Vol 51 (3) ◽

pp. 225-233 ◽

Cited By ~ 4

Author(s):

Safaei Mahdi ◽

Jafarnia Sasan ◽

Khosroshahi Sara

Keyword(s):

Colorado Potato Beetle ◽

Leptinotarsa Decemlineata ◽

Developmental Stages ◽

Linalyl Acetate ◽

Potential Control ◽

Oxygenated Monoterpenes ◽

Potato Beetle ◽

Leptinotarsa Decemlineata Say ◽

Different Populations ◽

Different Doses

Contact Toxicities of Oxygenated Monoterpenes to Different Populations of Colorado Potato Beetle,Leptinotarsa DecemlineataSay (Coleoptera: Chrysomelidae)In the present study, 12 pure oxygenated monoterpenes at 2 different doses were tested for their toxicity against second and third instar larvae and adults of three different populations of Colorado potato beetle (Leptinotarsa decemlineataSay). Some of tested compounds were found to be toxic to larvae and adults, but the degree of toxicity was variable. The mortality range was 20-100%. In general, fenchone, linalool, citronella and menthone showed a strong toxicity against the tested developmental stages; camphor, carvone and linalyl acetate showed moderate toxicity against larvae and adults of Colorado potato beetle and some compounds like fenchol, isomenthol, menthol, nerol and neryl acetate showed the least or no toxicity against the tested developmental stages ofL. decemlineata.Another important result was that although the tested populations of Colorado potato beetle showed some resistance to Endosulfan (50% WP), there was no resistance to tested oxygenated monoterpenes. The present results indicate that some of these compounds can be used as potential control agents against both larvae and adults of Colorado potato beetle.

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