Altitudinal Assessment ofAmblyomma aureolatumandAmblyomma ovale(Acari: Ixodidae), Vectors of Spotted Fever Group Rickettsiosis in the State of São Paulo, Brazil

2015 ◽  
Vol 52 (5) ◽  
pp. 1170-1174 ◽  
Author(s):  
Jonata M. Barbieri ◽  
Christiane M. B. M. Da Rocha ◽  
Fábio Raphael P. Bruhn ◽  
Dênis L. Cardoso ◽  
Adriano Pinter ◽  
...  
2005 ◽  
Vol 57 (3) ◽  
pp. 321-325 ◽  
Author(s):  
M.C. Horta ◽  
A. Pinter ◽  
A. Cortez ◽  
R.M. Soares ◽  
S.M. Gennari ◽  
...  

Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90%) and four fleas from Mogi das Cruzes (28%) were positive for the 17-kDa gene, and eight fleas from Pedreira (80%) and four from Mogi das Cruzes (28%) were positive for 190-kDa gene. The nucleotide sequence of the 190-kDa products of one flea from Pedreira and one flea from Mogi das Cruzes were 100% identical to each other, and when compared to the GenBank Data, they were 100% identical to the 190-kDa sequence of R. felis. This was the first report of its occurrence in the State of São Paulo.


1996 ◽  
Vol 91 (6) ◽  
pp. 701-702 ◽  
Author(s):  
Elba Regina Sampaio de Lemos ◽  
Raimundo Diogo Machado ◽  
José Rodrigues Coura ◽  
Maria Angélica Arpon Guimarães ◽  
Nicolau Maués Serra Freire

2008 ◽  
Vol 24 (2) ◽  
pp. 247-252 ◽  
Author(s):  
Adriano Pinter ◽  
Maurício C. Horta ◽  
Richard C. Pacheco ◽  
Jonas Moraes-Filho ◽  
Marcelo B. Labruna

The present study provides a rickettsial serosurvey in 25 dogs and 35 humans in an endemic area for Brazilian spotted fever in the State of São Paulo, where the tick Amblyomma aureolatum is the main vector. Testing canine and human sera by indirect immunofluorescence against four Rickettsia antigens (R. rickettsii, R. parkeri, R. felis and R. bellii) showed that 16 (64%) of canine sera and 1 (2.8%) of human sera reacted to at least one of these rickettsial antigens with titers <FONT FACE=Symbol>³</FONT> 64. Seven canine sera and the single reactive human serum showed titers to R. rickettsii at least four times those of any of the other three antigens. The antibody titers in these 7 animals and 1 human were attributed to stimulation by R. rickettsii infection. No positive canine or human serum was attributed to stimulation by R. parkeri, R. felis, or R. bellii. Our serological results showed that dogs are important sentinels for the presence of R. rickettsii in areas where the tick A. aureolatum is the main vector of Brazilian spotted fever.


1996 ◽  
Vol 38 (6) ◽  
pp. 427-430 ◽  
Author(s):  
Elba R.S. de Lemos ◽  
Raimundo D. Machado ◽  
José R. Coura ◽  
Maria A.A.M. Guimarães ◽  
Nelson Chagas

In order to obtain information on Brazilian spotted fever, a study in domestic animals was performed in the County of Pedreira, State of São Paulo, Brazil, where 17 human cases had been notified. Serum samples obtained from animals were tested by indirect immunofluorescence for detectable antibodies to spotted fever-group rickettsiae. Seropositivity was revealed in 12 (36.4%) of 33 dogs and seven (77.8%) of nine horses from the endemic area. For comparison, blood samples from dogs and horses from non endemic area were tested and four (12.9%) of 31 dogs and three (27.3%) of 11 horses were positive. The highest titers of antibodies by IFA (IgG > 1:1024) were found only in three dogs and six horses from endemic area. The results suggest that dogs as horses may serve as environmental sentinels for estabilishing the prevalence of foci of spotted fever in Brazil.


2013 ◽  
Vol 20 (1) ◽  
pp. 54-56 ◽  
Author(s):  
Flávia S. Gehrke ◽  
Rodrigo N. Angerami ◽  
Mauro T. Marrelli ◽  
Eliana R. de Souza ◽  
Elvira M.M. do Nascimento ◽  
...  

2020 ◽  
Vol 67 (6) ◽  
pp. 629-636
Author(s):  
Claudia Mello Ribeiro ◽  
Veruska Maia Costa ◽  
Jonas Lotufo Brant Carvalho ◽  
Rodrigo Guerrero Mendes ◽  
Paula Andrea de Santis Bastos ◽  
...  

2005 ◽  
Vol 100 (3) ◽  
pp. 277-279 ◽  
Author(s):  
Elvira Maria Mendes Nascimento ◽  
Flávia de Sousa Gehrke ◽  
Rosa Amélia Maldonado ◽  
Silvia Colombo ◽  
Luiz Jacintho da Silva ◽  
...  

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2543
Author(s):  
Filipe Corrêa Pacheco ◽  
Jonas Moraes Filho ◽  
Vlamir José Rocha ◽  
Bruno Rodrigues Sampieri ◽  
Melissa Marchi Zaniolo ◽  
...  

Spotted fever is a typically endemic infectious disease caused by rickettsiae from the spotted fever group, of which Rickettsia rickettsii is the main etiologic agent. It presents high mortality rates in Brazil, with transmission to humans or animals through the bite of infected ticks. The capybara (Hydrochoerus hydrochaeris) is an important reservoir for Rickettsia spp.; these bacteria can circulate in an infected animal presenting only fever as a clinical sign of the disease, as demonstrated by experimental infection. Considering the high zoonotic potential and the damage caused to human, animal, and environmental health, this study searched for anti-Rickettsia rickettsii antibodies in capybaras from an agricultural landscape in the city of Araras, State of São Paulo, Brazil. The indirect immunofluorescence (IFA) technique was used to detect anti-R. rickettsii antibodies. From the 28 serum samples tested using IFA, 18 (64.28%) were considered reactive, with antibody titers ranging from 256 to 2048. Seven (38.88%) samples presented titers of 256, three (16.67%) with titers of 512, five (27.78%) with titers of 1024, and three (16.67%) with titers of 2048. However, it was not possible to significantly associate gender to these serologic results. These results demonstrate that at some point during their lives, the studied capybaras were exposed to the etiologic agent, but it is impossible to know when this occurred. Further studies need to be performed to clarify which serological titers ensure an infection in capybaras, based on clinical and laboratory assessment of rickettsemia, and to establish the relationship between titers and the chronicity of disease. This is necessary owing to the possibility of cross-reactions with other rickettsiae species of the same subgroup, leading to the need for molecular tests to confirm diagnosis.


2009 ◽  
Vol 9 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Jonas Moraes-Filho ◽  
Adriano Pinter ◽  
Richard C. Pacheco ◽  
Thais B. Gutmann ◽  
Sonia O. Barbosa ◽  
...  

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