Polymerase Chain Reaction Detection Efficiency of the Human Granulocytic Ehrlichiosis Agent (Rickettsiaceae: Ehrlichieae) in Ticks (Acari: Ixodidae) is Dependent on the DNA Extraction Method

1999 ◽  
Vol 36 (6) ◽  
pp. 649-652 ◽  
Author(s):  
Michael J. Mauel ◽  
Stacey J. Carlton ◽  
Thomas N. Mather
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Detection Efficiency ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Granulocytic Ehrlichiosis ◽  
Dna Extraction Method ◽  
Human Granulocytic Ehrlichiosis ◽  
Polymerase Chain

scholarly journals A Rapid and Inexpensive Method for the Purification of DNA from Lichens and their Symbionts

1995 ◽  
Vol 27 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Daniele Armaleo ◽  
Philippe Clerc
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Restriction Enzymes ◽  
Inexpensive Method ◽  
Chain Reaction ◽  
Dna Extraction Method ◽  
Polymerase Chain

AbstractA simple DNA extraction method is described, applicable to many different kinds of lichens. The method involves the use of the detergents DTAB and CTAB and yields DNA that can be directly amplified with the polymerase chain reaction or digested with restriction enzymes

scholarly journals Effects of DNA extraction method on Porcine circovirus-2 real-time polymerase chain reaction quantification in swine lymph node samples

2011 ◽  
Vol 23 (6) ◽  
pp. 1189-1196 ◽  
Author(s):  
Silvia Faccini ◽  
Arrigo D. Nigrelli ◽  
Giuliana Franzini ◽  
Carlo Rosignoli ◽  
Ilaria Barbieri ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Real Time ◽  
Dna Extraction ◽  
Extraction Method ◽  
Porcine Circovirus ◽  
Quantitative Real Time Pcr ◽  
Chain Reaction ◽  
Dna Extraction Method ◽  
Polymerase Chain

Quantitative real-time polymerase chain reaction (PCR) has become an important tool for Porcine circovirus-2 (PCV-2) research and diagnosis. However, significant differences in detection limit and quantification data, among laboratories and quantitative real-time PCR methods, have been demonstrated. New efforts are required for providing more accurate and comparable results. The current study is an evaluation of the effects of DNA extraction procedures on PCV-2 quantification in lymph node samples. Differences, greater than 1 log10 copies/g, were shown among PCV-2 loads detected after different extraction procedures. The work highlighted the critical role of the DNA extraction method in PCV-2 quantification by quantitative real-time PCR. This important aspect should be evaluated when comparing data from different laboratories or different studies. The PCV-2 quantification data should not be considered comparable before demonstrating the equivalence of the DNA extraction methods performed.

scholarly journals Detection of Human Granulocytic Ehrlichiosis Agent and Borrelia Burgdorferi in Ticks by Polymerase Chain Reaction

1998 ◽  
Vol 10 (1) ◽  
pp. 56-59 ◽  
Author(s):  
Yung-Fu Chang ◽  
Vesna Novosel ◽  
Chao-Fu Chang ◽  
Jong Bae Kim ◽  
Sang J. Shin ◽  
...  
Keyword(s):  
New York ◽  
Polymerase Chain Reaction ◽  
Borrelia Burgdorferi ◽  
Laboratory Diagnosis ◽  
Etiologic Agent ◽  
Ixodid Ticks ◽  
Chain Reaction ◽  
Granulocytic Ehrlichiosis ◽  
Human Granulocytic Ehrlichiosis ◽  
Polymerase Chain

Adult ixodid ticks were collected from Westchester County, New York, and Ipswich, Massachusetts, to determine the presence of infection with a human granulocytic ehrlichiosis (HGE) agent by using the polymerase chain reaction (PCR). The presence of Borrelia burgdorferi in ticks collected from New York was also determined by PCR. Of the 229 ticks from New York and 47 ticks from Massachusetts, 9% (22/229) and 25% (12/47) of ticks contained HGE agent, respectively. Fifty-four percent (123/229) of the ticks collected from New York were B. burgdorferi positive; 4% (9/229) of these ticks contained both HGE agent and B. burgdorferi. This finding indicates that animals with Lyme borreliosis may be also exposed to the etiologic agent of HGE. More extensive laboratory diagnosis may be necessary when multiple tick-borne diseases are suspected in animals.

scholarly journals PERBANDINGAN METODE EKSTRAKSI DNA PHENOL-CHLOROFORM DAN KIT EXTRACTION PADA SAPI ACEH DAN SAPI MADURA

2017 ◽  
Vol 5 (1) ◽  
pp. 60
Author(s):  
Kamaliah Kamaliah
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Lower Price ◽  
Chain Reaction ◽  
Chloroform Extraction ◽  
Polymerase Chain ◽  
Electrophoresis Technique

DNA extract is the first phase of molecular research which really influences the quality of DNA isolate. The aim of this study was to find out the comparison of DNA extract by using Phenol-Chloroform and KIT extraction. The visualization of DNA isolate resulted from electrophoresis technique after leptin gen of Aceh’s cow and Madura’s cow were amplificated through in vitro (Polymerase Chain Reaction). There were not any techniques of DNA extraction which can provide excellence results as a whole. Phenol-Chloroform extraction method gives advantage for lower price, more sensitive in isolating DNA, and clear DNA ribbon. Meanwhile, KIT extraction method is easier, fast, and thick DNA produced.

scholarly journals Detection of the agent of human granulocytic ehrlichiosis (HGE) in UK ticks using polymerase chain reaction

1998 ◽  
Vol 121 (3) ◽  
pp. 681-683 ◽  
Author(s):  
E. GUY ◽  
S. TASKER ◽  
D. H. M. JOYNSON
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Presentation ◽  
Concurrent Infection ◽  
Chain Reaction ◽  
Granulocytic Ehrlichiosis ◽  
South Wales ◽  
Human Granulocytic Ehrlichiosis ◽  
Tick Borne Disease ◽  
Polymerase Chain ◽  
The Uk

Nymphal Ixodes ricinus ticks collected from woodland areas in South Wales, UK, were tested using the polymerase chain reaction for the presence both of the causative agent of human granulocytic ehrlichiosis (HGE) and Borrelia burgdorferi. Twenty-two of 60 (37%) ticks were found positive in the PCR for B. burgdorferi and 4/60 (7%) for the HGE agent. One tick was found positive both for B. burgdorferi and HGE agent. Our findings imply the presence of the HGE agent in UK ticks and the finding of a tick apparently containing both pathogens underlines the potential for concurrent infection with HGE agent and B. burgdorferi to occur after a single tick-bite. Based on our observations, we conclude that there may be a need to consider a range of pathogens both in laboratory investigation and clinical management of suspected tick-borne disease in the UK, particularly where there is a clinical presentation atypical of Lyme borreliosis alone.

scholarly journals Comparison of DNA Concentration and Purity of Animal Blood Extracted using Different DNA Extraction Kits

2018 ◽  
Vol 2 ◽  
Author(s):  
Mohamed Sabri Esa ◽  
Nur Huda Faujan ◽  
Haitham Abdullah Rajab ◽  
Maryam Mohamed Rehan ◽  
Norlelawati Arifin ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Bos Taurus ◽  
Dna Purification ◽  
Chain Reaction ◽  
Animal Blood ◽  
Dna Concentration ◽  
Dna Extraction Method ◽  
Polymerase Chain

The efficiency of DNA extraction from whole blood using appropriate method is very important for molecular analysis. Therefore, the aim of this study was to compare the purity and concentration of DNA extraction method from bovine (Bos taurus), chicken (Gallus gallus), and porcine (Sus scrofa) blood. The DNA of blood samples was extracted using three types of kit, namely InnuPREP Blood DNA Mini Kit, Wizard Genomic DNA Purification Kit, and QIAamp DNA Blood Mini Kit. The results showed that blood DNA extracted using QIAamp DNA Blood Mini Kit was found to be the most effective and consistently produced high concentrated and pure DNA for three animal samples. The purity of DNA ranged from 1.73 ± 0.05 Å to 1.94 ± 0.21 Å and the range of blood DNA concentration extracted using the QIAamp DNA were between 13.73 ± 2.11 and 25.01 ± 2.08 ng/?l. However, the blood DNA of porcine was not successfully extracted using InnuPREP Blood DNA Mini Kit and Wizard® Genomic DNA Purification Kit. These results were very crucial for the subsequent use of amplification using polymerase chain reaction (PCR) and to facilitate accurate detection in further analysis.

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