scholarly journals A simple and rapid DNA extraction method from leaves of grapevine suitable for polymerase chain reaction analysis

2012 ◽  
Vol 11 (45) ◽  
Author(s):  
Sandra Lo Piccolo
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Dna Extraction Method ◽  
Polymerase Chain

scholarly journals A Rapid and Inexpensive Method for the Purification of DNA from Lichens and their Symbionts

1995 ◽  
Vol 27 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Daniele Armaleo ◽  
Philippe Clerc
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Restriction Enzymes ◽  
Inexpensive Method ◽  
Chain Reaction ◽  
Dna Extraction Method ◽  
Polymerase Chain

AbstractA simple DNA extraction method is described, applicable to many different kinds of lichens. The method involves the use of the detergents DTAB and CTAB and yields DNA that can be directly amplified with the polymerase chain reaction or digested with restriction enzymes

scholarly journals Effects of DNA extraction method on Porcine circovirus-2 real-time polymerase chain reaction quantification in swine lymph node samples

2011 ◽  
Vol 23 (6) ◽  
pp. 1189-1196 ◽  
Author(s):  
Silvia Faccini ◽  
Arrigo D. Nigrelli ◽  
Giuliana Franzini ◽  
Carlo Rosignoli ◽  
Ilaria Barbieri ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Real Time ◽  
Dna Extraction ◽  
Extraction Method ◽  
Porcine Circovirus ◽  
Quantitative Real Time Pcr ◽  
Chain Reaction ◽  
Dna Extraction Method ◽  
Polymerase Chain

Quantitative real-time polymerase chain reaction (PCR) has become an important tool for Porcine circovirus-2 (PCV-2) research and diagnosis. However, significant differences in detection limit and quantification data, among laboratories and quantitative real-time PCR methods, have been demonstrated. New efforts are required for providing more accurate and comparable results. The current study is an evaluation of the effects of DNA extraction procedures on PCV-2 quantification in lymph node samples. Differences, greater than 1 log10 copies/g, were shown among PCV-2 loads detected after different extraction procedures. The work highlighted the critical role of the DNA extraction method in PCV-2 quantification by quantitative real-time PCR. This important aspect should be evaluated when comparing data from different laboratories or different studies. The PCV-2 quantification data should not be considered comparable before demonstrating the equivalence of the DNA extraction methods performed.

Sex identification of pigeons using polymerase chain reaction analysis with simple DNA extraction

2019 ◽  
Vol 12 (2) ◽  
pp. 45-48
Author(s):  
Shao-jie Liang ◽  
Ming-xia Chen ◽  
Chun-qi Gao ◽  
Hui-chao Yan ◽  
Guo-long Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Polymerase Chain Reaction Analysis ◽  
Sex Identification ◽  
Z Chromosome ◽  
W Chromosome ◽  
Chain Reaction ◽  
Assay Time ◽  
Polymerase Chain ◽  
The Cost

Sex identification plays an important role in avian production. Hitherto, it is difficult to distinguish the sexes of monomorphic birds based on their external features. The chromo-helicase-DNA-binding genes contain CHD-W gene and CHD-Z gene, which are located on the W chromosome and Z chromosome, respectively. Since CHD-W gene is unique to females, the polymerase chain reaction can be used for sex identification. However, extracting DNA procedures for verifying the sex is tedious and expensive. To address these disadvantages, the objective of this study was to develop a simple DNA extraction assay to efficiently process blood, liver, and feather samples. The results showed that 2% dimethylsulfoxide was suitable for processing blood, and phosphate-buffered saline was suitable for processing liver and feather samples. The specific primers were designed, and the length of the targets is 474 bp on Z chromosome and 319 bp on W chromosome. The pigeons were identified as females based on the presence of two bands on the gel, and as males based on the presence of one band. Taken together, our results suggested that feather samples were more appropriate than blood or liver for sex identification of pigeons. Compared to the traditional DNA extraction, this method shortened the assay time and reduced the cost.

scholarly journals Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis

2014 ◽  
Vol 13 (3) ◽  
pp. 6070-6078 ◽  
Author(s):  
E.C.B. Silva ◽  
M.A. Pelinca ◽  
A.C. Acosta ◽  
D.M.F. Silva ◽  
M.A. Gomes Filho ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Comparative Study ◽  
Dna Extraction ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Erratum to: Evaluation of Five Probiotic Products for Label Claims by DNA Extraction and Polymerase Chain Reaction Analysis

2005 ◽  
Vol 50 (6) ◽  
pp. 1117-1117 ◽  
Author(s):  
Jeanne Drisko ◽  
Bette Bischoff ◽  
Cheryl Giles ◽  
Martin E. Adelson ◽  
Raja-Venkitesh S. Rao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

Evaluation of Five Probiotic Products for Label Claims by DNA Extraction and Polymerase Chain Reaction Analysis

2005 ◽  
Vol 50 (6) ◽  
pp. 1113-1117 ◽  
Author(s):  
Jeanne Drisko ◽  
Bette Bischoff ◽  
Cheryl Giles ◽  
Martin Adelson ◽  
Raja-Venkitesh S. Rao ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals PERBANDINGAN METODE EKSTRAKSI DNA PHENOL-CHLOROFORM DAN KIT EXTRACTION PADA SAPI ACEH DAN SAPI MADURA

2017 ◽  
Vol 5 (1) ◽  
pp. 60
Author(s):  
Kamaliah Kamaliah
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Lower Price ◽  
Chain Reaction ◽  
Chloroform Extraction ◽  
Polymerase Chain ◽  
Electrophoresis Technique

DNA extract is the first phase of molecular research which really influences the quality of DNA isolate. The aim of this study was to find out the comparison of DNA extract by using Phenol-Chloroform and KIT extraction. The visualization of DNA isolate resulted from electrophoresis technique after leptin gen of Aceh’s cow and Madura’s cow were amplificated through in vitro (Polymerase Chain Reaction). There were not any techniques of DNA extraction which can provide excellence results as a whole. Phenol-Chloroform extraction method gives advantage for lower price, more sensitive in isolating DNA, and clear DNA ribbon. Meanwhile, KIT extraction method is easier, fast, and thick DNA produced.

scholarly journals Comparison of DNA Concentration and Purity of Animal Blood Extracted using Different DNA Extraction Kits

2018 ◽  
Vol 2 ◽  
Author(s):  
Mohamed Sabri Esa ◽  
Nur Huda Faujan ◽  
Haitham Abdullah Rajab ◽  
Maryam Mohamed Rehan ◽  
Norlelawati Arifin ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Bos Taurus ◽  
Dna Purification ◽  
Chain Reaction ◽  
Animal Blood ◽  
Dna Concentration ◽  
Dna Extraction Method ◽  
Polymerase Chain

The efficiency of DNA extraction from whole blood using appropriate method is very important for molecular analysis. Therefore, the aim of this study was to compare the purity and concentration of DNA extraction method from bovine (Bos taurus), chicken (Gallus gallus), and porcine (Sus scrofa) blood. The DNA of blood samples was extracted using three types of kit, namely InnuPREP Blood DNA Mini Kit, Wizard Genomic DNA Purification Kit, and QIAamp DNA Blood Mini Kit. The results showed that blood DNA extracted using QIAamp DNA Blood Mini Kit was found to be the most effective and consistently produced high concentrated and pure DNA for three animal samples. The purity of DNA ranged from 1.73 ± 0.05 Å to 1.94 ± 0.21 Å and the range of blood DNA concentration extracted using the QIAamp DNA were between 13.73 ± 2.11 and 25.01 ± 2.08 ng/?l. However, the blood DNA of porcine was not successfully extracted using InnuPREP Blood DNA Mini Kit and Wizard® Genomic DNA Purification Kit. These results were very crucial for the subsequent use of amplification using polymerase chain reaction (PCR) and to facilitate accurate detection in further analysis.

Sign in / Sign up

Export Citation Format

Share Document