scholarly journals A Rapid and Inexpensive Method for the Purification of DNA from Lichens and their Symbionts

1995 ◽  
Vol 27 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Daniele Armaleo ◽  
Philippe Clerc
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Restriction Enzymes ◽  
Inexpensive Method ◽  
Chain Reaction ◽  
Dna Extraction Method ◽  
Polymerase Chain

AbstractA simple DNA extraction method is described, applicable to many different kinds of lichens. The method involves the use of the detergents DTAB and CTAB and yields DNA that can be directly amplified with the polymerase chain reaction or digested with restriction enzymes

scholarly journals Effects of DNA extraction method on Porcine circovirus-2 real-time polymerase chain reaction quantification in swine lymph node samples

2011 ◽  
Vol 23 (6) ◽  
pp. 1189-1196 ◽  
Author(s):  
Silvia Faccini ◽  
Arrigo D. Nigrelli ◽  
Giuliana Franzini ◽  
Carlo Rosignoli ◽  
Ilaria Barbieri ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Real Time ◽  
Dna Extraction ◽  
Extraction Method ◽  
Porcine Circovirus ◽  
Quantitative Real Time Pcr ◽  
Chain Reaction ◽  
Dna Extraction Method ◽  
Polymerase Chain

Quantitative real-time polymerase chain reaction (PCR) has become an important tool for Porcine circovirus-2 (PCV-2) research and diagnosis. However, significant differences in detection limit and quantification data, among laboratories and quantitative real-time PCR methods, have been demonstrated. New efforts are required for providing more accurate and comparable results. The current study is an evaluation of the effects of DNA extraction procedures on PCV-2 quantification in lymph node samples. Differences, greater than 1 log10 copies/g, were shown among PCV-2 loads detected after different extraction procedures. The work highlighted the critical role of the DNA extraction method in PCV-2 quantification by quantitative real-time PCR. This important aspect should be evaluated when comparing data from different laboratories or different studies. The PCV-2 quantification data should not be considered comparable before demonstrating the equivalence of the DNA extraction methods performed.

scholarly journals PERBANDINGAN METODE EKSTRAKSI DNA PHENOL-CHLOROFORM DAN KIT EXTRACTION PADA SAPI ACEH DAN SAPI MADURA

2017 ◽  
Vol 5 (1) ◽  
pp. 60
Author(s):  
Kamaliah Kamaliah
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Extraction Method ◽  
Lower Price ◽  
Chain Reaction ◽  
Chloroform Extraction ◽  
Polymerase Chain ◽  
Electrophoresis Technique

DNA extract is the first phase of molecular research which really influences the quality of DNA isolate. The aim of this study was to find out the comparison of DNA extract by using Phenol-Chloroform and KIT extraction. The visualization of DNA isolate resulted from electrophoresis technique after leptin gen of Aceh’s cow and Madura’s cow were amplificated through in vitro (Polymerase Chain Reaction). There were not any techniques of DNA extraction which can provide excellence results as a whole. Phenol-Chloroform extraction method gives advantage for lower price, more sensitive in isolating DNA, and clear DNA ribbon. Meanwhile, KIT extraction method is easier, fast, and thick DNA produced.

scholarly journals Comparison of DNA Concentration and Purity of Animal Blood Extracted using Different DNA Extraction Kits

2018 ◽  
Vol 2 ◽  
Author(s):  
Mohamed Sabri Esa ◽  
Nur Huda Faujan ◽  
Haitham Abdullah Rajab ◽  
Maryam Mohamed Rehan ◽  
Norlelawati Arifin ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Bos Taurus ◽  
Dna Purification ◽  
Chain Reaction ◽  
Animal Blood ◽  
Dna Concentration ◽  
Dna Extraction Method ◽  
Polymerase Chain

The efficiency of DNA extraction from whole blood using appropriate method is very important for molecular analysis. Therefore, the aim of this study was to compare the purity and concentration of DNA extraction method from bovine (Bos taurus), chicken (Gallus gallus), and porcine (Sus scrofa) blood. The DNA of blood samples was extracted using three types of kit, namely InnuPREP Blood DNA Mini Kit, Wizard Genomic DNA Purification Kit, and QIAamp DNA Blood Mini Kit. The results showed that blood DNA extracted using QIAamp DNA Blood Mini Kit was found to be the most effective and consistently produced high concentrated and pure DNA for three animal samples. The purity of DNA ranged from 1.73 ± 0.05 Å to 1.94 ± 0.21 Å and the range of blood DNA concentration extracted using the QIAamp DNA were between 13.73 ± 2.11 and 25.01 ± 2.08 ng/?l. However, the blood DNA of porcine was not successfully extracted using InnuPREP Blood DNA Mini Kit and Wizard® Genomic DNA Purification Kit. These results were very crucial for the subsequent use of amplification using polymerase chain reaction (PCR) and to facilitate accurate detection in further analysis.

scholarly journals Method Comparison of DNA Isolation and Quantification for Fish and Seafood Authenticity Determination

2018 ◽  
Vol 13 (3) ◽  
pp. 115
Author(s):  
Dwiyitno Dwiyitno ◽  
Stefan Hoffman ◽  
Koen Parmentier ◽  
Chris Van Keer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Dna Isolation ◽  
Blue Mussel ◽  
Pcr Amplification ◽  
Amplification Efficiency ◽  
Chain Reaction ◽  
Seafood Products ◽  
Polymerase Chain ◽  
Commercial Kit

Fish and seafood products has been commonly targeted for fraudulent activities. For that reason, authentication of fish and seafood products is important to protect consumers from fraudulent and adulteration practices, as well as to implement traceability regulation. From the viewpoint of food safety, authenticity is beneficial to protect public from serious food poisoning incidents, such as due to ingestion of toxic species. Since DNA based identification depends on the nucleic acid polymerase chain reaction (PCR), the quantity and quality/purity of DNA will contribute significantly to the species authentication. In the present study, different DNA extraction and purification methods (3 classical methods and one commercial kit) were compared to produce the better isolated DNA for PCR amplification. Additionally, different methods for the estimation of DNA concentration and purity which is essential for PCR amplification efficiency were also evaluated. The result showed that classical DNA extraction methods (based on TNES-Urea) yielded a higher amount of DNA (11.30-323.60 ng/g tissue) in comparison to commercial kit/Wizard Promega (5.70-83.45 ng/g tissue). Based on the purity of DNA extract (A260/280), classical DNA extraction method produced relatively similar on DNA quality to the commercial kit (1.79-2.12). Interestingly, all classical methods produced DNA with A260/280 ratio of more than 2.00 on the blue mussel, in contrast with commercial kit. The commercial kit also produced better quality of DNA compared to the classical methods, showing the higher efficiency in PCR amplification. NanoDrop is promising as cheap, robust and safe UV-spectrophotometer method for DNA quantification, as well as the purity evaluation.Keywords: seafood authenticity, DNA isolation, polymerase chain reaction, NanoDrop, Picogreen

scholarly journals Molecular diagnosis of the curly lettuce parasitic contamination from hydroponic cultivation from supermarkets

2020 ◽  
Vol 29 (4) ◽  
Author(s):  
Fernanda Pinto-Ferreira ◽  
Jonatan Batista Reis ◽  
Aline Ticiani Pereira Paschoal ◽  
Letícia Santos Balbino ◽  
Amanda Bertão-Santos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Dna Extraction ◽  
Chain Reaction ◽  
Cryptosporidium Spp ◽  
Hydroponic Cultivation ◽  
Polymerase Chain ◽  
Fluctuation Method ◽  
The City ◽  
Commercial Kit

Abstract The consumption of vegetables has increased in recent years due to the search for a healthier diet that is rich in fiber and has fewer calories. To assess the parasitic contamination of lettuce sold in markets, a survey of parasites was carried out from a supermarket chain in the city of Londrina, Paraná. A total of thirty samples of lettuce were purchased in the ten markets visited, three in each, of which ten were conventionally cultivated, ten were hydroponically cultivated, and ten were organically cultivated. All samples were analyzed using the sedimentation methods of Hoffman, Pons and Janer and the fluctuation method of Faust and colleagues and Willis with adaptations. In addition, the samples were subjected to DNA extraction by a commercial kit and polymerase chain reaction to detect Toxoplasma gondii, Cryptosporidium spp. and Giardia spp., which are protozoa that cause food and waterborne parasitic outbreaks. All samples were negative for sedimentation and flotation techniques. One of the hydroponically cultivated samples was positive for T. gondii. The results demonstrate the risk of curly lettuce contamination from hydroponic cultivation and the need for proper cleaning of these foods before consumption.

scholarly journals Molecular detection and occurrence of equine theileriosis in Arabian horses in Al-Najaf province/Iraq

2020 ◽  
Vol 57 (3) ◽  
pp. e166996
Author(s):  
Hayder Mohammad Al-Rammahi ◽  
Abdulameer Abed Hatem ◽  
Asaad Chasib Al-Atabi
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Molecular Detection ◽  
Molecular Technique ◽  
Chain Reaction ◽  
Blood Samples ◽  
Equine Piroplasmosis ◽  
Polymerase Chain

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.

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