Ultra-structural observation of human enamel and dentin by ultra-high-voltage electron tomography and the focus ion beam technique

2012 ◽  
Vol 61 (5) ◽  
pp. 335-341 ◽  
Author(s):  
J. Miura ◽  
M. Kubo ◽  
T. Nagashima ◽  
F. Takeshige
Keyword(s):  
High Voltage ◽  
Electron Tomography ◽  
Ion Beam ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Human Enamel ◽  
Focus Ion Beam ◽  
Beam Technique ◽  
Ultra High Voltage

High-Voltage Electron Tomography of the Early C. elegans Mitotic Spindle

2003 ◽  
Vol 9 (S03) ◽  
pp. 384-385
Author(s):  
Eileen T. O'Toole ◽  
Kent L. McDonald ◽  
Jana Mäntler ◽  
J. Richard McIntosh ◽  
Anthony A. Hyman ◽  
...  
Keyword(s):  
High Voltage ◽  
Mitotic Spindle ◽  
Electron Tomography ◽  
C Elegans ◽  
Voltage Electron ◽  
High Voltage Electron

scholarly journals Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans

2003 ◽  
Vol 163 (3) ◽  
pp. 451-456 ◽  
Author(s):  
Eileen T. O'Toole ◽  
Kent L. McDonald ◽  
Jana Mäntler ◽  
J. Richard McIntosh ◽  
Anthony A. Hyman ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Dynamic Behavior ◽  
High Voltage ◽  
Electron Tomography ◽  
In Vitro Studies ◽  
Embryonic Cells ◽  
Nematode Caenorhabditis Elegans ◽  
Voltage Electron ◽  
High Voltage Electron

During mitosis, the connections of microtubules (MTs) to centrosomes and kinetochores are dynamic. From in vitro studies, it is known that the dynamic behavior of MTs is related to the structure of their ends, but we know little about the structure of MT ends in spindles. Here, we use high-voltage electron tomography to study the centrosome- and kinetochore-associated ends of spindle MTs in embryonic cells of the nematode, Caenorhabditis elegans. Centrosome-associated MT ends are either closed or open. Closed MT ends are more numerous and are uniformly distributed around the centrosome, but open ends are found preferentially on kinetochore-attached MTs. These results have structural implications for models of MT interactions with centrosomes.

scholarly journals The Largest Possible Microscope

1994 ◽  
Vol 2 (5) ◽  
pp. 10-10
Author(s):  
Sterling P. Newberry
Keyword(s):  
High Voltage ◽  
Focal Length ◽  
Big Science ◽  
Distinguishing Characteristic ◽  
Voltage Electron ◽  
Science Projects ◽  
High Voltage Electron ◽  
Ordinary Light ◽  
Electron Microscopes ◽  
Ultra High Voltage

In this day of resistance toward big science projects it may be futile to worry about how to produce the largest possible microscope. But since it doesn't cost anything to day dream, and times may change, it is fun to consider superlative enterprises.One must grant that Ultra High Voltage Electron Microscopes are much larger than we wish they were, and one wonders if there is a contest afoot to see which AEM can have the largest foot print, but a new possibility has arisen which, seriously, could dwarf them all - yet have some possible use.I am referring to the discovery of an example of a gravitation lens for ordinary light. Now the distinguishing characteristic of a microscope from a telescope is that the object eyepiece and the object near the focal length of the lens.

An autofocus method using quasi-Gaussian fitting of image sharpness in ultra-high-voltage electron microscopy

Microscopy ◽  
2013 ◽  
Vol 62 (5) ◽  
pp. 515-519 ◽  
Author(s):  
R. Nishi ◽  
Y. Moriyama ◽  
K. Yoshida ◽  
N. Kajimura ◽  
H. Mogaki ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Image Sharpness ◽  
High Voltage Electron Microscopy ◽  
Gaussian Fitting ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Ultra High Voltage
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