Oncogenicity in Marmosets of HL-23V, a Type C Oncornavirus Isolated From Human Leukemic Cells, and Comparison With Simian Sarcoma Virus Type 1 (SSV-1/SSAV-1)
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Herein, we present the case of a patient who suffered from adult T-cell leukemia/lymphoma (ATLL) and hepatocellular carcinoma (HCC) after obtaining a sustained virological response following treatment with a direct-acting antiviral (DAA) at different points in time. The patient went into complete remission (CR) for ATLL. Unfortunately, subsequent relapse of ATLL was observed. This situation was overcome using chemotherapy with pegylated interferon alpha-2b. Human T lymphotropic virus type 1 Tax-specific cytotoxic T lymphocytes (CTLs) were recognized after obtaining second CR, and those CTLs have been maintained for many years. After 4 years from the second CR, chronic hepatitis type C was treated with a DAA, and sustained virological response was attained. However, the occurrence of HCC was detected. Surprisingly, the tumor disappeared spontaneously. Hepatitis virus type C-specific CTLs were also detected in the patient. T-cell receptor (TCR) V beta gene repertoire analyses revealed oligoclonal expansion of effector and memory CTLs. The number of CTLs expressing the TCR V beta 13.1 has increased over the years since HCC occurrence. The activation and maintenance of anticancer cellular immunity may have allowed the patient to obtain long-term survival and overcome two lethal neoplasms.

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Studies of Simian Sarcoma and Simian Sarcoma-Associated Virus: I. Analysis of Viral Structural Proteins, and Preparation and Characterization of Antiserum Specific for Viral Envelope Components

Zeitschrift für Naturforschung C ◽

10.1515/znc-1978-11-1227 ◽

1978 ◽

Vol 33 (11-12) ◽

pp. 969-980 ◽

Cited By ~ 15

Author(s):

F. Deinhardt ◽

C. Bergholz ◽

G. Hunsmann ◽

J. Schneider ◽

H.-J. Thiel ◽

...

Keyword(s):

Viral Envelope ◽

Structural Proteins ◽

Coomassie Blue ◽

Sarcoma Virus ◽

Simian Sarcoma Virus ◽

A Minor ◽

Leukemia Viruses

The structural proteins of simian sarcoma virus type 1 and its associated virus (SSV) were analysed; in general they were similar to the corresponding components of murine leukemia viruses (MuLV), i. e. glycoproteins of approximately 69.000 and 71.000 Daltons (gp69/71), proteins corresponding to p15(E) and p12(E) which were identified as envelope, and p31, p15, p12 and p10 proteins identified as internal components. As in MuLV, p12 stained reddish with Coomassie blue but a pl5(C) distinct from p15(E) was not clearly identified. Using antisera specific for individual components of MuLV cross-reactions were observed between the proteins pl5(E), pi2(E) and p31 of SSV and MuLV, and to a minor degree also between their respective major glycoproteins. An antiserum reacting strongly, specifically and almost exclusively with the envelope components of SSV gp69/71, p15 (E) and p12 (E) was prepared in a goat by inoculation of the animal’s own cells, previously transformed in vitro with SSV and grown in goat serum. Comparative studies with this antiserum in complement fixation and Ouchterlony tests confirmed the strong antigenic similarities between SSV and gibbon ape lymphoma virus but did not identify any interspecies reactivity.

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