Need for Confirmatory Neutralization Tests for Hepatitis B Surface Antigen Tests in Populations with Intermediate Prevalence

2021 ◽  
Author(s):  
Min Young Lee ◽  
So Young Kang ◽  
Woo In Lee ◽  
Myeong Hee Kim
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Neutralization Test ◽  
Hepatitis B Surface Antigen ◽  
False Negative ◽  
Confirmatory Test ◽  
Negative Group ◽  
Positive Group ◽  
Test Kit ◽  
Antigen Tests

Abstract Objective Hepatitis B surface antigen (HBsAg) is known as the hallmark of hepatitis B virus (HBV) infection. This study aimed to determine whether an HBsAg neutralization test is necessary to accurately interpret HBsAg test results. Methods Initially reactive HBsAg specimens from a 5-year period, with cutoff index values between 1.0 and 2.0, were subjected to neutralization confirmatory testing using an Elecsys HBsAg Confirmatory test kit (Roche Diagnostics GmbH. Mannheim, Germany). Results The neutralization test showed 46.1% positive (confirmed positive group) and 53.9% negative (confirmed negative group) results from the total specimens. Among the confirmed negative group, 79.5% of patients were confirmed to be negative for the current infection, whereas 4 patients in the chronic hepatitis B subgroup showed a neutralization percentage close to 40%. More than half of patients in the confirmed positive group were considered to be in the hepatitis B e antigen-negative inactive HBsAg carrier phase. Conclusion In populations with intermediate HBV prevalence, a neutralization test is necessary to confirm an HBsAg result and reduce the false positive and false negative rates of initial HBsAg tests.

scholarly journals One Example of False Negative Hepatitis B Surface Antigen (EIA) Result Due to Variant S Area Strain and Reagment Reactiveness Related to Hepatitis B Surface Antigen

2011 ◽  
Vol 85 (1) ◽  
pp. 21-25
Author(s):  
Chikashi MATSUDA ◽  
Hidehiko MORIYAMA ◽  
Takeshi TAKETANI ◽  
Hiroshi SHIBATA ◽  
Atsushi NAGAI
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
False Negative ◽  
Area Strain

Erratum to “Performance of hepatitis B surface antigen tests with the first WHO international hepatitis B virus genotype reference panel” [J. Clin. Virol. 58 (2013) 47–53]

2013 ◽  
Vol 58 (3) ◽  
pp. 598-599
Author(s):  
M. Chudy ◽  
H. Scheiblauer ◽  
K.-M. Hanschmann ◽  
J. Kress ◽  
S. Nick ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Reference Panel ◽  
Virus Genotype ◽  
B Virus ◽  
Antigen Tests

Proposal of Efficient Workflows for Confirmatory Neutralization Test for Initial Hepatitis B Surface Antigen Positive Samples

2019 ◽  
Vol 65 (10/2019) ◽  
Author(s):  
Soo-Kyung Kim ◽  
Jungwon Huh ◽  
Tae-Dong Jeong
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Neutralization Test ◽  
Hepatitis B Surface Antigen

Performance of hepatitis B surface antigen tests with the first WHO international hepatitis B virus genotype reference panel

2013 ◽  
Vol 58 (1) ◽  
pp. 47-53 ◽  
Author(s):  
M. Chudy ◽  
H. Scheiblauer ◽  
K.-M. Hanschmann ◽  
J. Kress ◽  
S. Nick ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Reference Panel ◽  
Virus Genotype ◽  
B Virus ◽  
Antigen Tests

scholarly journals Notes from the Field: False-Negative Hepatitis B Surface Antigen Test Results in a Hemodialysis Patient — Nebraska, 2017

2018 ◽  
Vol 67 (10) ◽  
pp. 311-312 ◽  
Author(s):  
Blake Hendrickson ◽  
Saleem Kamili ◽  
Tim Timmons ◽  
Peter C. Iwen ◽  
Caitlin Pedati ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hemodialysis Patient ◽  
Hepatitis B Surface Antigen ◽  
False Negative ◽  
Test Results ◽  
Antigen Test

scholarly journals Seroprevalence of hepatitis B virus amongst patients attending selected hospitals in Niger State, Nigeria

2021 ◽  
Vol 01 (01) ◽  
Author(s):  
Aisha Adamu ◽  
Faruk Kuta
Keyword(s):  
Hepatitis B Virus ◽  
Blood Transfusion ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Hbv Infection ◽  
Awareness Campaigns ◽  
Test Kit ◽  
B Virus ◽  
History Of

Hepatitis B virus (HBV) is a major public health problem globally and accounts for about one million deaths worldwide annually. This study determined the seroprevalence, distribution of HBV infection, and factors associated with the infection amongst patients attending selected hospitals in Niger State. A total of 500 blood samples were collected from five selected hospitals in Niger state. The samples were screened using Hepatitis B Surface Antigen (HBsAg) test kit for the qualitative detection of Hepatitis B Surface Antigen in serum. Prevalence of Hepatitis B infection was 13.0% cumulatively in the study area. Female participants had a higher prevalence (6.8%) of HBsAg infection compared to their male counterparts with 6.2%. Participants within the 41- 50 years’ age group recorded a higher rate of infection (5.2%), while those ≥ 50 years had a lower prevalence of (2.3%). The civil servants had a higher percentage prevalence of 6.4% followed by housewives and the least was observed with participants who are students. Patients without a history of blood transfusion recorded a higher percentage prevalence (7.8%) compared to those with a history of blood transfusion (5.2%). The results reveal that participants with polygamous family types recorded a higher prevalence of HBV infection (11.4%) compared to those belonging to the monogamous type (1.6%). The results obtained from this study suggest that HBV is in circulation in the study areas; thus necessitating more awareness campaigns among the general population about HBV and its modes of transmission and associated risk factors

scholarly journals False-Negative Hepatitis B Virus (HBV) Surface Antigen in a Vaccinated Dialysis Patient with a High Level of HBV DNA in the United States

2012 ◽  
Vol 19 (5) ◽  
pp. 820-822 ◽  
Author(s):  
Matthew C. Foy ◽  
Chloe L. Thio ◽  
Hyon S. Hwang ◽  
Melissa Saulynas ◽  
James P. Hamilton ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hemodialysis Patient ◽  
Hepatitis B Surface Antigen ◽  
False Negative ◽  
The United States ◽  
Hbv Dna ◽  
B Virus ◽  
High Level

ABSTRACTScreening with hepatitis B surface antigen (HBsAg) is highly recommended for at-risk individuals. Mutations in the HBsAg can result in an inability to detect the virus during routine screening. We describe a hemodialysis patient found to have high levels of hepatitis B virus (HBV) DNA and HBV antibody but negative HBsAg on two routine assays.

scholarly journals Positive hepatitis B surface antigen tests due to recent vaccination: a persistent problem

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Carolyn D Rysgaard ◽  
Cory S Morris ◽  
Denny Drees ◽  
Tami Bebber ◽  
Scott R Davis ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Persistent Problem ◽  
Antigen Tests

scholarly journals Performance of a New-Generation Chemiluminescent Assay for Hepatitis B Surface Antigen

2006 ◽  
Vol 52 (8) ◽  
pp. 1592-1598 ◽  
Author(s):  
Dan Chen ◽  
Lawrence A Kaplan
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
False Negative ◽  
Negative Results ◽  
False Negative Results ◽  
Serologic Markers ◽  
Hbsag Assay ◽  
New Generation ◽  
Dna Pcr

Abstract Background: The usual criteria for analysis of hepatitis B surface antigen (HBsAg) are detection of HBsAg and result confirmation by antibody neutralization. We observed that with the Immulite 2000 HBsAg assay [Diagnostics Product Corporation (DPC)] a relatively high percentage of weakly reactive (WR) samples did not pass the neutralization step. Methods: For each of 3 lots of Immulite 2000 HBsAg reagent (DPC), we collected and analyzed HBsAg data from ∼3000 to 4000 patient blood samples and compared these data with HBsAg data from 3393 samples tested with the Abbott Auszyme assay. For 127 samples with initially WR detection signals (relative signal/cutoff index of 1.00–2.5) on the Immulite 2000 HBsAg assay, we then measured hepatitis B (HB) viral load and/or other HB serologic markers. Results: The Immulite 2000 HBsAg assay produced more initially reactive results than the Abbott Auszyme method. Many of these reactive samples, however, were WR and did not meet the confirmation criteria in the neutralization test. Moreover, DNA PCR testing indicated that 22 of the 38 WR samples (58%) that did meet the confirmation criteria had no detectable HB viral DNA. Conclusions: Immulite 2000 HBsAg assay results include a unique group of WR samples that are associated with both false-positive and false-negative results, regardless of neutralization status, and require careful interpretation. WR HBsAg samples should be reported as confirmed HBsAg reactive only if the samples not only meet the neutralization criteria but also are positive for other HB serologic markers such as anti-HB core total and anti-HB core IgM.

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