Abstract
Background
Development of a tebipenem antimicrobial susceptibility test was completed for the MicroScan Dried Gram-negative MIC (MSDGN) Panel when compared to CLSI broth microdilution reference panels.
Methods
Development was conducted by comparing MICs obtained using the MSDGN panel to MICs using a CLSI broth microdilution reference panel. A total of 669 Enterobacterales isolates were tested at 16, 18, and 20 hour incubation times using the turbidity and Prompt®* methods of inoculation. MSDGN panels were incubated at 35 ± 2ºC and read on the WalkAway System, the autoSCAN-4 instrument, and read visually. Frozen reference panels, prepared according to ISO/CLSI methodology, were inoculated using the turbidity inoculation method. All frozen reference panels were incubated at 35 ± 2ºC and read visually. Dilution sequence evaluated is 0.03-32 µg/mL.
Results
When compared to frozen reference panel results, essential agreement for all isolates tested during development are as follows:
Conclusion
The development data showed that tebipenem MIC results for Enterobacterales obtained with the MSDGN panel correlate well with MICs obtained using frozen reference panels. Essential agreement is > 90% for all inoculation and read methods.
For Investigational Use Only. The performance characteristics of this product have not been established.
* Prompt® is a registered trademark of 3M Company, St. Paul, MN USA.
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Disclosures
Jose Enrique Fernandez, n/a, Beckman Coulter (Employee) Vasna Carr, n/a, Beckman Coulter (Employee) Renae Miller, n/a, Beckman Coulter (Employee)