scholarly journals Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene

1985 ◽  
Vol 13 (20) ◽  
pp. 7237-7256 ◽  
Author(s):  
Bret A.M. Morris-Krsinich ◽  
Philip M. Mullineaux ◽  
Jonathan Donson ◽  
Margaret I. Boulton ◽  
Peter G. Markham ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Maize Streak Virus ◽  
Streak Virus ◽  
Bidirectional Transcription

scholarly journals Identification of Tobacco streak virus in Cranberry and the Association of TSV with Berry Scarring

Plant Disease ◽  
2016 ◽  
Vol 100 (4) ◽  
pp. 696-703 ◽  
Author(s):  
L. D. Wells-Hansen ◽  
J. J. Polashock ◽  
N. Vorsa ◽  
B. E. L. Lockhart ◽  
P. S. McManus
Keyword(s):  
New Jersey ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Management Strategies ◽  
Tobacco Streak Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Streak Virus ◽  
Transmission Electron ◽  
Gene Sequence Analysis

Cranberry plants bearing disfigured, scarred fruit were reported by growers in the major cranberry-growing region of central Wisconsin in July 2012. Plants bearing scarred fruit have since been observed in Massachusetts and New Jersey. Three complementary methods provided evidence of Tobacco streak virus (TSV) in symptomatic plants: (i) leaves and scarred berries tested positive for TSV by double-antibody sandwich enzyme-linked immunosorbent assay; (ii) quasi-isometric particles approximately 33 nm in diameter were extracted from leaves of symptomatic plants and visualized using transmission electron microscopy; and (iii) coat protein gene sequence analysis revealed 94 to 99% nucleotide similarity with reference TSV sequences. In newer cultivars, 99% of uprights with scarred berries tested positive for TSV. In older cultivars, 31% of uprights with scarred berries tested positive for TSV and the remaining 69% of uprights with scarred berries tested positive for Blueberry shock virus. TSV overwintered in cranberry plants, and leaves, pollen, and fruit tested positive for TSV the year following symptom occurrence. Attempts to inoculate cranberry using infected pollen or sap as inoculum failed, but several herbaceous hosts tested TSV positive following mechanical inoculation. Phylogenetic analysis of the coat protein gene of 26 TSV isolates from various cultivars of cranberry in Wisconsin, New Jersey, and Massachusetts revealed diversity. This work provides information that will be useful in understanding the epidemiology of TSV in cranberry and in the development of management strategies.

scholarly journals New species emergence via recombination among isolates of the Brazilian tomato infecting Begomovirus complex

2006 ◽  
Vol 41 (8) ◽  
pp. 1329-1332 ◽  
Author(s):  
Alice Kazuko Inoue-Nagata ◽  
Darren Patrick Martin ◽  
Leonardo Silva Boiteux ◽  
Leonardo de Britto Giordano ◽  
Isabel Cristina Bezerra ◽  
...  
Keyword(s):  
New Species ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Intergenic Region ◽  
Protein Gene ◽  
Nucleotide Sequences ◽  
Yellow Vein ◽  
Streak Virus ◽  
Recombination Analysis ◽  
Leaf Curl Virus

Partial nucleotide sequences of five tomato infecting Begomovirus isolates were determined from DNA-A fragments, corresponding to the 5' region of the replication associated protein gene, the intergenic region and the 5' region of the coat protein gene. Isolate DFM shared 95% identity with Tomato mottle leaf curl virus (TMoLCV), isolates 34, PA-05, and Ta4 were 88% identical to Tomato yellow vein streak virus and isolate DF-BR3 shared 77% identity with TMoLCV. Recombination analysis indicated that isolate DF-BR3 was a chimaera, and it provided evidence that there is a complex and actively recombining population of tomato infecting begomoviruses in Brazil.

scholarly journals Nucleotide sequence of the potato leafroll virus coat protein gene

1989 ◽  
Vol 17 (4) ◽  
pp. 1768-1768 ◽  
Author(s):  
B. Prill ◽  
E. Maiss ◽  
U. Timpe ◽  
R. Casper
Keyword(s):  
Nucleotide Sequence ◽  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Potato Leafroll Virus ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Leafroll Virus

scholarly journals Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil

2011 ◽  
Vol 36 (2) ◽  
pp. 121-124 ◽  
Author(s):  
José Evando A. Beserra Jr. ◽  
Eduardo C. Andrade ◽  
Rosa F.R. Araújo Camarço ◽  
Aline K.Q. Nascimento ◽  
José Albérsio A. Lima
Keyword(s):  
Coat Protein ◽  
Mosaic Virus ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Northeastern Brazil ◽  
Sequence Variability ◽  
Virus Isolates

scholarly journals Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil

2002 ◽  
Vol 27 (3) ◽  
pp. 279-284 ◽  
Author(s):  
OSMAR NICKEL ◽  
THOR V. M. FAJARDO ◽  
FRANCISCO J. L. ARAGÃO ◽  
CÉSAR M. CHAGAS ◽  
GILMAR B. KUHN
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Amino Acid Sequences ◽  
Grapevine Virus ◽  
Leaf Extracts ◽  
Western Blots ◽  
Gene Characterization ◽  
Gene Coding ◽  
Grapevine Virus B

An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific primers for GVB, a fragment of 594 bp, comprising the coat protein gene coding for 197 amino acids, was amplified by RT-PCR with viral RNA extracted from GVB-infected N. occidentalis. The nucleotide and the deduced amino acid sequences of the coat protein gene showed high identities with Italian and Japanese isolates of GVB.

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