Close association of a DNA replication origin and anARSelement on chromosome III of the yeast,Saccharomyces cerevisiae

Two-dimensional gel electrophoretic replicon mapping techniques were used to identify all functional DNA replication origins and termini in a 26.5-kbp stretch in the left arm of yeast chromosome III. Only one origin was detected; it coincided with an ARS element (ARS306), as have all previously mapped yeast origins. A replication termination region was identified in a 4.3-kbp stretch at the telomere-proximal end of the investigated region, between the origin identified in this paper and the neighboring, previously mapped, ARS305-associated origin (previously called the A6C origin). Termination does not occur at a specific site; instead, it appears to be the consequence of replication forks converging in a stretch of DNA of at least 4.3 kbp.

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Chromosomal DNA replication initiates at the same origins in meiosis and mitosis

Molecular and Cellular Biology ◽

10.1128/mcb.14.5.3524-3534.1994 ◽

1994 ◽

Vol 14 (5) ◽

pp. 3524-3534

Author(s):

I Collins ◽

C S Newlon

Keyword(s):

Saccharomyces Cerevisiae ◽

Dna Replication ◽

S Phase ◽

Replication Origins ◽

Chromosomal Dna ◽

Chromosomal Replication ◽

Yeast Saccharomyces Cerevisiae ◽

Autonomously Replicating Sequence ◽

Ars Elements ◽

Chromosome Iii

Autonomously replicating sequence (ARS) elements are identified by their ability to promote high-frequency transformation and extrachromosomal replication of plasmids in the yeast Saccharomyces cerevisiae. Six of the 14 ARS elements present in a 200-kb region of Saccharomyces cerevisiae chromosome III are mitotic chromosomal replication origins. The unexpected observation that eight ARS elements do not function at detectable levels as chromosomal replication origins during mitotic growth suggested that these ARS elements may function as chromosomal origins during premeiotic S phase. Two-dimensional agarose gel electrophoresis was used to map premeiotic replication origins in a 100-kb segment of chromosome III between HML and CEN3. The pattern of origin usage in premeiotic S phase was identical to that in mitotic S phase, with the possible exception of ARS308, which is an inefficient mitotic origin associated with CEN3. CEN3 was found to replicate during premeiotic S phase, demonstrating that the failure of sister chromatids to disjoin during the meiosis I division is not due to unreplicated centromeres. No origins were found in the DNA fragments without ARS function. Thus, in both mitosis and meiosis, chromosomal replication origins are coincident with ARS elements but not all ARS elements have chromosomal origin function. The efficiency of origin use and the patterns of replication termination are similar in meiosis and in mitosis. DNA replication termination occurs over a broad distance between active origins.

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Chromosomal DNA replication initiates at the same origins in meiosis and mitosis.

Molecular and Cellular Biology ◽

10.1128/mcb.14.5.3524 ◽

1994 ◽

Vol 14 (5) ◽

pp. 3524-3534 ◽

Cited By ~ 37

Author(s):

I Collins ◽

C S Newlon

Keyword(s):

Saccharomyces Cerevisiae ◽

Dna Replication ◽

S Phase ◽

Replication Origins ◽

Chromosomal Dna ◽

Chromosomal Replication ◽

Yeast Saccharomyces Cerevisiae ◽

Autonomously Replicating Sequence ◽

Ars Elements ◽

Chromosome Iii

Autonomously replicating sequence (ARS) elements are identified by their ability to promote high-frequency transformation and extrachromosomal replication of plasmids in the yeast Saccharomyces cerevisiae. Six of the 14 ARS elements present in a 200-kb region of Saccharomyces cerevisiae chromosome III are mitotic chromosomal replication origins. The unexpected observation that eight ARS elements do not function at detectable levels as chromosomal replication origins during mitotic growth suggested that these ARS elements may function as chromosomal origins during premeiotic S phase. Two-dimensional agarose gel electrophoresis was used to map premeiotic replication origins in a 100-kb segment of chromosome III between HML and CEN3. The pattern of origin usage in premeiotic S phase was identical to that in mitotic S phase, with the possible exception of ARS308, which is an inefficient mitotic origin associated with CEN3. CEN3 was found to replicate during premeiotic S phase, demonstrating that the failure of sister chromatids to disjoin during the meiosis I division is not due to unreplicated centromeres. No origins were found in the DNA fragments without ARS function. Thus, in both mitosis and meiosis, chromosomal replication origins are coincident with ARS elements but not all ARS elements have chromosomal origin function. The efficiency of origin use and the patterns of replication termination are similar in meiosis and in mitosis. DNA replication termination occurs over a broad distance between active origins.

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Localization of a DNA replication origin and termination zone on chromosome III of Saccharomyces cerevisiae

Molecular and Cellular Biology ◽

10.1128/mcb.12.10.4733-4741.1992 ◽

1992 ◽

Vol 12 (10) ◽

pp. 4733-4741

Author(s):

J Zhu ◽

C S Newlon ◽

J A Huberman

Keyword(s):

Dna Replication ◽

Replication Origin ◽

Replication Forks ◽

Two Dimensional ◽

Yeast Chromosome ◽

Dna Replication Origin ◽

Functional Dna ◽

Mapping Techniques ◽

Dna Replication Origins ◽

Chromosome Iii

Two-dimensional gel electrophoretic replicon mapping techniques were used to identify all functional DNA replication origins and termini in a 26.5-kbp stretch in the left arm of yeast chromosome III. Only one origin was detected; it coincided with an ARS element (ARS306), as have all previously mapped yeast origins. A replication termination region was identified in a 4.3-kbp stretch at the telomere-proximal end of the investigated region, between the origin identified in this paper and the neighboring, previously mapped, ARS305-associated origin (previously called the A6C origin). Termination does not occur at a specific site; instead, it appears to be the consequence of replication forks converging in a stretch of DNA of at least 4.3 kbp.

Download Full-text