scholarly journals Functional difference between the two oppositely oriented priming signals essential for the initiation of the broad host-range plasmid RSF1010 DNA replication

1994 ◽  
Vol 22 (5) ◽  
pp. 767-772 ◽  
Author(s):  
Katsunori Tanaka ◽  
Kouichi Kino ◽  
Yoshitomo Taguchi ◽  
Deng-Ming Miao ◽  
Yoichi Honda ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Broad Host Range ◽  
Functional Difference ◽  
Broad Host Range Plasmid

Multiple mechanisms for expression of incompatibility by broad-host-range plasmid RK2

1982 ◽  
Vol 152 (3) ◽  
pp. 1078-1090
Author(s):  
R Meyer ◽  
M Hinds
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Plasmid Dna ◽  
Plasmid Vector ◽  
Broad Host Range ◽  
Plasmid Maintenance ◽  
Broad Host Range Plasmid ◽  
Expression Of Genes ◽  
Plasmid Partitioning ◽  
Plasmid Rk2

By cloning fragments of plasmid DNA, we have shown that RK2 expresses incompatibility by more than one mechanism. One previously identified (R. J. Meyer, Mol. Gen, Genet. 177:155--161, 1979; Thomas et al., Mol. Gen. Genet. 181:1--7, 1981) determinant for incompatibility is linked to the origin of plasmid DNA replication. When cloned into a plasmid vector, this determinant prevents the stable inheritance of a coresident RK2. However, susceptibility to this mechanism of incompatibility requires an active RK2 replicon and is abolished if another replicator is provided. We have also cloned a second incompatibility determinant, encoded within the 54.1- to 56.4-kilobase region of RK2 DNA, which we call IncP-1(II). An RK2 derivative remains sensitive to IncP-1(II), even when it is not replicating by means of the RK2 replicon. The 54.1- to 56.4-kilobase DNA does not confer susceptibility to the IncP-1(II) mechanism, nor does it encode a detectable system for efficient plasmid partitioning. The incompatibility may be related to the expression of genes mapping in the 54.1- to 56.4-kilobase region, which are required for plasmid maintenance and suppression of plasmid-encoded killing functions.

scholarly journals Functional Distinction among Structural Subsections in the Specific Priming Signal for DNA Replication of the Broad Host-Range Plasmid RSF1010

1995 ◽  
Vol 59 (5) ◽  
pp. 920-921 ◽  
Author(s):  
Deng-Ming Miao ◽  
Hiroshi Sakai ◽  
Katsunori Tanaka ◽  
Yoichi Honda ◽  
Tohru Komano ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Specific Priming

scholarly journals A base-paired hairpin structure essential for the functional priming signal for DNA replication of the broad host range plasmid RSF1010

1993 ◽  
Vol 21 (21) ◽  
pp. 4900-4903 ◽  
Author(s):  
Dang-Ming Miao ◽  
Yoichi Honda ◽  
Katsunori Tanaka ◽  
Akihisa Higashi ◽  
Toshiaki Nakamura ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Hairpin Structure ◽  
Broad Host Range ◽  
Broad Host Range Plasmid

scholarly journals Mutational analysis of the specific priming signal essential for DNA replication of the broad host-range plasmid RSF1010

FEBS Letters ◽  
1993 ◽  
Vol 324 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Yoichi Honda ◽  
Tae Akioka ◽  
So Takebe ◽  
Katsunori Tanaka ◽  
Dengming Miao ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Mutational Analysis ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Specific Priming
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