The 20 bp, directly repeated DNA sequence of broad host range plasmid R1162 exerts incompatibility in vivo and inhibits R1162 DNA replication in vitro

1987 ◽  
Vol 208 (3) ◽  
pp. 390-397 ◽  
Author(s):  
Lung-Shen Lin ◽  
Yung-Jin Kim ◽  
Richard J. Meyer
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Dna Sequence ◽  
Broad Host Range ◽  
Repeated Dna ◽  
Broad Host Range Plasmid ◽  
Repeated Dna Sequence

scholarly journals IncC of Broad-Host-Range Plasmid RK2 Modulates KorB Transcriptional Repressor Activity In Vivo and Operator Binding In Vitro

1999 ◽  
Vol 181 (9) ◽  
pp. 2807-2815 ◽  
Author(s):  
Grazyna Jagura-Burdzy ◽  
Kalliope Kostelidou ◽  
Jessica Pole ◽  
Dheeraj Khare ◽  
Anthony Jones ◽  
...  
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Coordinate Regulation ◽  
Broad Host Range Plasmid ◽  
Reporter Systems ◽  
Operator Binding ◽  
Repressor Activity ◽  
Plasmid Rk2

ABSTRACT The korAB operon of broad-host-range plasmid RK2 encodes five genes, two of which, incC andkorB, belong to the parA and parBfamilies, respectively, of genome partitioning functions. BothkorB and a third gene, korA, are responsible for coordinate regulation of operons encoding replication, transfer, and stable inheritance functions. Overexpression of incCalone caused rapid displacement of RK2. Using two different reporter systems, we show that incC modulates the action of KorB. Using promoter fusions to the reporter gene xylE, we show that incC potentiates the repression of transcription bykorB. This modulation of korB activity was only observed with incC1, which encodes the full-length IncC (364 amino acids [aa]), whereas no effect was observed withincC2, which encodes a polypeptide of 259 aa that lacks the N-terminal 105 aa. Using bacterial extracts with IncC1 and IncC2 or IncC1 purified through the use of a His6 tail and Ni-agarose chromatography, we showed that IncC1 potentiates the binding of KorB to DNA at representative KorB operators. The ability of IncC to stabilize KorB-DNA complexes suggests that these two proteins work together in the global regulation of many operons on the IncP-1 genomes, as well in plasmid partitioning.

Multiple mechanisms for expression of incompatibility by broad-host-range plasmid RK2

1982 ◽  
Vol 152 (3) ◽  
pp. 1078-1090
Author(s):  
R Meyer ◽  
M Hinds
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Plasmid Dna ◽  
Plasmid Vector ◽  
Broad Host Range ◽  
Plasmid Maintenance ◽  
Broad Host Range Plasmid ◽  
Expression Of Genes ◽  
Plasmid Partitioning ◽  
Plasmid Rk2

By cloning fragments of plasmid DNA, we have shown that RK2 expresses incompatibility by more than one mechanism. One previously identified (R. J. Meyer, Mol. Gen, Genet. 177:155--161, 1979; Thomas et al., Mol. Gen. Genet. 181:1--7, 1981) determinant for incompatibility is linked to the origin of plasmid DNA replication. When cloned into a plasmid vector, this determinant prevents the stable inheritance of a coresident RK2. However, susceptibility to this mechanism of incompatibility requires an active RK2 replicon and is abolished if another replicator is provided. We have also cloned a second incompatibility determinant, encoded within the 54.1- to 56.4-kilobase region of RK2 DNA, which we call IncP-1(II). An RK2 derivative remains sensitive to IncP-1(II), even when it is not replicating by means of the RK2 replicon. The 54.1- to 56.4-kilobase DNA does not confer susceptibility to the IncP-1(II) mechanism, nor does it encode a detectable system for efficient plasmid partitioning. The incompatibility may be related to the expression of genes mapping in the 54.1- to 56.4-kilobase region, which are required for plasmid maintenance and suppression of plasmid-encoded killing functions.

scholarly journals Functional Distinction among Structural Subsections in the Specific Priming Signal for DNA Replication of the Broad Host-Range Plasmid RSF1010

1995 ◽  
Vol 59 (5) ◽  
pp. 920-921 ◽  
Author(s):  
Deng-Ming Miao ◽  
Hiroshi Sakai ◽  
Katsunori Tanaka ◽  
Yoichi Honda ◽  
Tohru Komano ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Specific Priming

Conjugative Transfer and in Vitro/in Vivo Stability of the Broad-Host-Range IncP R751 Plasmid in Brucella spp.

Plasmid ◽  
1993 ◽  
Vol 29 (2) ◽  
pp. 142-146 ◽  
Author(s):  
J.M. Verger ◽  
M. Grayon ◽  
E. Chaslus-Dancla ◽  
M. Meurisse ◽  
J.P. Lafont
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Conjugative Transfer

scholarly journals Functional difference between the two oppositely oriented priming signals essential for the initiation of the broad host-range plasmid RSF1010 DNA replication

1994 ◽  
Vol 22 (5) ◽  
pp. 767-772 ◽  
Author(s):  
Katsunori Tanaka ◽  
Kouichi Kino ◽  
Yoshitomo Taguchi ◽  
Deng-Ming Miao ◽  
Yoichi Honda ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Broad Host Range ◽  
Functional Difference ◽  
Broad Host Range Plasmid

In Vivo and in Vitro Stability of the Broad-Host-Range Cloning Vector pBBR1MCS in Six Brucella Species

Plasmid ◽  
1995 ◽  
Vol 33 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Philip H. Elzer ◽  
Michael E. Kovach ◽  
Robert W. Phillips ◽  
Gregory T. Robertson ◽  
Kenneth M. Peterson ◽  
...  
Keyword(s):  
Host Range ◽  
Cloning Vector ◽  
Broad Host Range ◽  
Brucella Species ◽  
In Vitro Stability

scholarly journals A base-paired hairpin structure essential for the functional priming signal for DNA replication of the broad host range plasmid RSF1010

1993 ◽  
Vol 21 (21) ◽  
pp. 4900-4903 ◽  
Author(s):  
Dang-Ming Miao ◽  
Yoichi Honda ◽  
Katsunori Tanaka ◽  
Akihisa Higashi ◽  
Toshiaki Nakamura ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Hairpin Structure ◽  
Broad Host Range ◽  
Broad Host Range Plasmid

scholarly journals Mutational analysis of the specific priming signal essential for DNA replication of the broad host-range plasmid RSF1010

FEBS Letters ◽  
1993 ◽  
Vol 324 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Yoichi Honda ◽  
Tae Akioka ◽  
So Takebe ◽  
Katsunori Tanaka ◽  
Dengming Miao ◽  
...  
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Mutational Analysis ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Specific Priming

Generation in vitro of deletions in the broad host range plasmid RK2 using phage Mu insertions and a restriction endonuclease

Gene ◽  
1976 ◽  
Vol 1 (1) ◽  
pp. 107-119 ◽  
Author(s):  
David Figurski ◽  
Richard Meyer ◽  
Daniel S. Miller ◽  
Donald R. Helinski
Keyword(s):  
Host Range ◽  
Restriction Endonuclease ◽  
Broad Host Range ◽  
Phage Mu ◽  
Broad Host Range Plasmid ◽  
Plasmid Rk2

scholarly journals Role of TrfA and DnaA Proteins in Origin Opening during Initiation of DNA Replication of the Broad Host Range Plasmid RK2

1997 ◽  
Vol 272 (32) ◽  
pp. 20173-20178 ◽  
Author(s):  
Igor Konieczny ◽  
Kelly S. Doran ◽  
Donald R. Helinski ◽  
Alessandra Blasina
Keyword(s):  
Dna Replication ◽  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Initiation Of Dna Replication ◽  
Plasmid Rk2
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