scholarly journals Development of new DNA triplex triads by using 5-substituted deoxycytidine

2009 ◽  
Vol 53 (1) ◽  
pp. 163-164
T. Kanamori ◽  
H. Tsunoda ◽  
A. Ohkubo ◽  
M. Sekine ◽  
K. Seio
2020 ◽  
Vol 20 (16) ◽  
pp. 1943-1955
Neelam Lohani ◽  
Moganty R. Rajeswari

Background: The high mobility group box 1 (hmgb1) is one of the frequently over-expressed genes whose aberrant expression is reported in a number of human cancers. Various strategies are underway to inhibit hmgb1 expression in cancer cells having considerable therapeutic value. Objective: The present work involves selective transcriptional inhibition of the hmgb1 gene using selective DNA triplex structure-based gene technology. Here, the promoter region of the hmgb1 gene at position (-183 to -165) from the transcription start site as a target was selected using bioinformatic tools. Methods: The DNA triplex formation by the DNA of the target gene and TFO was confirmed using UV absorption spectroscopy, Circular Dichroism, and Isothermal Calorimetry. Results: Treatment of HepG2 cell with specific Triplex-forming Oligonucleotide significantly downregulated HMGB1 expression level at mRNA and protein levels by 50%, while the classical anticancer drugs, actinomycin/ adriamycin as positive controls showed 65% and the combination of TFO and drug decreased by 70%. The anti-proliferative effects of TFO correlated well with the fact of accumulation of cells in the Go phase and apoptotic cell death. Further, the binding of anti-cancer drugs to hmgb1 is stronger in DNA triplex state as compared to hmgb1 alone, suggesting the combination therapy as a better option. Conclusion: Therefore, the ability of hmgb1 targeted triplex-forming oligonucleotide in combination with triplex selective anticancer drug holds promise in the treatment of malignancies associated with hmgb1 overexpression. The result obtained may open up new vistas to provide a basis for the rational drug design and searching for high-affinity ligands with a high triplex selectivity.

Biochemistry ◽  
1994 ◽  
Vol 33 (14) ◽  
pp. 4111-4120 ◽  
Karen Dittrich ◽  
Juan Gu ◽  
Robert Tinder ◽  
Michael Hogan ◽  
Xiaolian Gao

ChemInform ◽  
2001 ◽  
Vol 32 (5) ◽  
pp. no-no
Pascal Savy ◽  
Rachid Benhida ◽  
Jean-Louis Fourrey ◽  
Rosalie Maurisse ◽  
Jian-Sheng Sun

2020 ◽  
Kevin Jahnke ◽  
Noah Ritzmann ◽  
Julius Fichtler ◽  
Anna Nitschke ◽  
Yannik Dreher ◽  

Abstract Bottom-up and top-down approaches to synthetic biology each employ distinct methodologies with the common aim to harness new types of living systems. Both approaches, however, face their own challenges towards biotechnological and biomedical applications. Here, we realize a strategic merger to convert light into proton gradients for the actuation of synthetic cellular systems. We genetically engineer E. coli to overexpress the light-driven inward-directed proton pump xenorhodopsin and encapsulate them as organelle mimics in artificial cell-sized compartments. Exposing the compartments to light-dark cycles, we can reversibly switch the pH by almost one pH unit and employ these pH gradients to trigger the attachment of DNA structures to the compartment periphery. For this purpose, a DNA triplex motif serves as a nanomechanical switch responding to the pH-trigger of the E. coli. By attaching a polymerized DNA origami plate to the DNA triplex motif, we obtain a cytoskeleton mimic that considerably deforms lipid vesicles in a pH-responsive manner. We foresee that the combination of bottom-up and top down approaches is an efficient way to engineer synthetic cells as potent microreactors.

1992 ◽  
Vol 114 (1) ◽  
pp. 357-359 ◽  
Iris Dieter-Wurm ◽  
Michal Sabat ◽  
Bernhard Lippert

2014 ◽  
Vol 464 (2) ◽  
pp. 203-211 ◽  
Weronika Kotkowiak ◽  
Michał Kotkowiak ◽  
Ryszard Kierzek ◽  
Anna Pasternak

UNA moieties within the TFO strongly destabilize triplexes. Introduction of UNA into specific positions in the hairpin structure is energetically favourable for triplex formation. UNA increases the resistance of the oligonucleotides to serum nucleases when incorporated at specific hairpin positions.

2014 ◽  
Vol 6 (5) ◽  
pp. 3513-3519 ◽  
Lingyan Feng ◽  
Zhijun Zhang ◽  
Jinsong Ren ◽  
Xiaogang Qu

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