scholarly journals Cyclosporin A affects extracellular matrix synthesis and degradation by mouse MC3T3‐E1 osteoblasts in vitro

2001 ◽  
Vol 16 (3) ◽  
pp. 500-505 ◽  
Author(s):  
Alessia Fornoni ◽  
Flavia Cornacchia ◽  
Guy A. Howard ◽  
Bernard A. Roos ◽  
Gary E. Striker ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cyclosporin A ◽  
Matrix Synthesis ◽  
Synthesis And Degradation

Gene expression, cytoskeletal changes and extracellular matrix synthesis in human osteoblasts treated with cyclosporin A

2009 ◽  
Vol 63 (9) ◽  
pp. 619-626 ◽  
Author(s):  
Maurizio Vertemati ◽  
Ernesto Minola ◽  
Claudia Dolci ◽  
Giordano Stabellini ◽  
Furio Pezzetti ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Cyclosporin A ◽  
Matrix Synthesis ◽  
Human Osteoblasts

Icariin Promotes Extracellular Matrix Synthesis and Gene Expression of Chondrocytes In Vitro

2012 ◽  
Vol 26 (9) ◽  
pp. 1385-1392 ◽  
Author(s):  
Lei Zhang ◽  
Xuan Zhang ◽  
Kui-Feng Li ◽  
Dong-Xiao Li ◽  
Yu-Mei Xiao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Matrix Synthesis

scholarly journals LncRNA GAS5 as miR-26a-5p Sponge Regulates the PTEN/PI3K/Akt Axis and Affects Extracellular Matrix Synthesis in Degenerative Nucleus Pulposus Cells in vitro

2021 ◽  
Vol 12 ◽  
Author(s):  
Liang Tan ◽  
Yifang Xie ◽  
Ye Yuan ◽  
Kai Hu
Keyword(s):  
Extracellular Matrix ◽  
Nucleus Pulposus ◽  
Intervertebral Disc Degeneration ◽  
Nucleus Pulposus Cell ◽  
Nucleus Pulposus Cells ◽  
Akt Inhibitor ◽  
Matrix Synthesis ◽  
Lncrna Gas5

The role of lncRNA growth arrest specific 5 (GAS5) in degenerative nucleus pulposus cell (NPC) apoptosis has been reported, but the mechanism of GAS5 in extracellular matrix (ECM) synthesis in intervertebral disc degeneration (IDD) remains unknown. We aimed to investigate the mechanism of GAS5 in ECM synthesis in degenerative NPCs. GAS5 expression was measured in degenerative NPCs (CP-H170) and normal NPCs (CP-H097). siRNA-mediated GAS5 knockdown was transfected to NPCs to detect cell viability and the expression of ECM-related genes (Collagen II, aggrecan, Collagen I, and MMP-3). Subcellular localization of GAS5 was analyzed. The downstream gene and pathway of GAS5 in degenerative NPCs were explored. As our results indicated, lncRNA GAS5 was upregulated in degenerative NPCs. Silencing GAS5 improved the viability of degenerative NPCs and increased ECM synthesis. GAS5 was mainly located in the cytoplasm of NPCs. LncRNA GAS5 sponged miR-26a-5p to regulate PTEN. Overexpression of miR-26a-5p promoted ECM synthesis in degenerative NPCs. Akt inhibitor LY294002 reversed the promotion of silencing GAS5 on ECM synthesis of degenerative NPCs. In conclusion, lncRNA GAS5 sponged miR-26a-5p to upregulate PTEN and inhibit the PI3K/Akt pathway, thus inhibiting ECM synthesis of degenerative NPCs.

Glucose Availability Affects Extracellular Matrix Synthesis During Chondrogenesis In Vitro

2021 ◽  
Author(s):  
Yi Zhong ◽  
Arnold I Caplan ◽  
Jean F. Welter ◽  
Harihara Baskaran
Keyword(s):  
Extracellular Matrix ◽  
Matrix Synthesis ◽  
Glucose Availability

Imbalance in Extracellular Matrix Synthesis and Degradation as a Mechanism of Leaflet Weakening in Sporadic Mitral Valve Prolapse in Humans

2012 ◽  
Vol 2012 (4) ◽  
pp. 84
Author(s):  
Samiya Hussain ◽  
Bryant V. McIver ◽  
Lexis Neill ◽  
Kim Biao ◽  
John D. Puskas ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Mitral Valve ◽  
Mitral Valve Prolapse ◽  
Matrix Synthesis ◽  
Synthesis And Degradation

Effect of Mechanical Forces on Extracellular Matrix Synthesis by Bovine Urethral Fibroblasts in Vitro

1993 ◽  
Vol 150 (2 Part 2) ◽  
pp. 637-641 ◽  
Author(s):  
Laurence Baskin ◽  
Pamela S. Howard ◽  
Edward Macarak
Keyword(s):  
Extracellular Matrix ◽  
Mechanical Forces ◽  
Matrix Synthesis

scholarly journals Prolonged Scar-in-a-Jar: an in vitro screening tool for anti-fibrotic therapies using biomarkers of extracellular matrix synthesis

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Sarah Rank Rønnow ◽  
Rand Qais Dabbagh ◽  
Federica Genovese ◽  
Carmel B. Nanthakumar ◽  
Vikki J. Barrett ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Screening Tool ◽  
In Vitro Screening ◽  
Matrix Synthesis
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