scholarly journals Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model

2004 ◽  
Vol 19 (7) ◽  
pp. 1742-1751 ◽  
Author(s):  
J. P. Faure ◽  
C. Jayle ◽  
D. Dutheil ◽  
M. Eugene ◽  
K. Zhang ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Renal Medulla ◽  
Cold Preservation ◽  
High Sodium ◽  
Pig Kidney ◽  
Sodium Solution

Protective Roles of Polyethylene Glycol and Trimetazidine against Cold Ischemia and Reperfusion Injuries of Pig Kidney Graft

2004 ◽  
Vol 4 (4) ◽  
pp. 495-504 ◽  
Author(s):  
Jean Pierre Faure ◽  
Isabelle Petit ◽  
Keqiang Zhang ◽  
Delphine Dutheil ◽  
Carole Doucet ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Ischemia And Reperfusion ◽  
Kidney Graft ◽  
Cold Ischemia ◽  
Pig Kidney

Influence of Retrieval Conditions on Renal Medulla Injury: Evaluation by Proton NMR Spectroscopy in an Isolated Perfused Pig Kidney Model

2000 ◽  
Vol 93 (1) ◽  
pp. 1-8 ◽  
Author(s):  
T. Hauet ◽  
H. Gibelin ◽  
J.P. Richer ◽  
C. Godart ◽  
M. Eugene ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Proton Nmr ◽  
Renal Medulla ◽  
Proton Nmr Spectroscopy ◽  
Pig Kidney

scholarly journals Corticomedullary Shunting After Ischaemia and Reperfusion in The Porcine Kidney?

2020 ◽  
Author(s):  
Michael Rehling ◽  
Stine Gram Skjøth ◽  
Anna Krarup Keller ◽  
Lene Elsebeth Nielsen ◽  
Christian Flø ◽  
...  
Keyword(s):  
Renal Cortex ◽  
Renal Medulla ◽  
Renal Perfusion ◽  
Visual Evaluation ◽  
Contralateral Kidney ◽  
Renal Uptake ◽  
Renal Ischaemia ◽  
Pig Kidney ◽  
Ischaemia And Reperfusion

Abstract Background: An animal model offers the opportunity to study organs in vivo and the porcine model was chosen to simulate a renal transplantation with complications. Renal perfusion may redistribute from cortex to medulla during systemic hypovolaemia and after renal ischaemia for other reasons, but there is no consensus on this matter. We studied renal perfusion after renal ischaemia and reperfusion.Methods: Renal perfusion distribution was examined by use of 153Gadolinium-labeled microspheres (MS) after 2 hours (hrs) and 4 hrs ischaemia of the pig kidney followed by 4 hrs of reperfusion. Intra-arterial injected MS are trapped in the glomeruli in renal cortex, which means that MS are not present in the medulla under normal physiological conditions.Results: Visual evaluation after reperfusion demonstrated that MS redistributed from the renal cortex to the medulla in 6 out of 16 pigs (38%) subjected to 4 hrs ischaemia and in one out of 18 pigs subjected to 2 hrs ischaemia. Central renal uptake of MS covering the medullary/total renal uptake was significantly higher in kidneys subjected to 4 hrs ischaemia compared with pigs subjected to 2 hrs ischaemia (69±5% vs. 63±1%, p<0.001), and also significantly higher than in the contralateral kidney (69±5% vs. 63±2, p<0.001). Analysis of blood and urine demonstrated no presence of radioactivity. Conclusion: The study demonstrated the presence of MS in the renal medulla in response to renal ischaemia and reperfusion suggesting that severe ischaemia and reperfusion of the pig kidney leads to opening of functional shunts bypassing glomeruli.

scholarly journals Renal medullary endothelin-1 is decreased in Dahl salt-sensitive rats

2011 ◽  
Vol 301 (2) ◽  
pp. R519-R523 ◽  
Author(s):  
Joshua S. Speed ◽  
Babbette LaMarca ◽  
Hunter Berry ◽  
Kathy Cockrell ◽  
Eric M. George ◽  
...  
Keyword(s):  
Sodium Intake ◽  
Renal Medulla ◽  
Receptor Expression ◽  
High Salt Diet ◽  
Salt Diet ◽  
High Sodium ◽  
Endothelin System ◽  
Chronic Infusion ◽  
Salt Sensitive Hypertension

Although it is well established that the renal endothelin (ET-1) system plays an important role in regulating sodium excretion and blood pressure through activation of renal medullary ETB receptors, the role of this system in Dahl salt-sensitive (DS) hypertension is unclear. The purpose of this study was to determine whether the DS rat has abnormalities in the renal medullary endothelin system when maintained on a high sodium intake. The data indicate that Dahl salt-resistant rats (DR) on a high-salt diet had a six-fold higher urinary endothelin excretion than in the DR rats with low Na+ intake (17.8 ± 4 pg/day vs. 112 ± 44 pg/day). In sharp contrast, urinary endothelin levels increased only twofold in DS rats in response to a high Na+ intake (13 ± 2 pg/day vs. 29.8 ± 5.5 pg/day). Medullary endothelin concentration in DS rats on a high-Na+ diet was also significantly lower than DR rats on a high-Na+ diet (31 ± 2.8 pg/mg vs. 70.9 ± 5 pg/mg). Furthermore, DS rats had a significant reduction in medullary ETB receptor expression compared with DR rats while on a high-Na+ diet. Finally, chronic infusion of ET-1 directly into the renal medulla blunted Dahl salt-sensitive hypertension. These data indicate that a decrease in medullary production of ET-1 in the DS rat could play an important role in the development of salt-sensitive hypertension observed in the DS rat.

Polyethylene Glycol Reduces Early and Long-Term Cold Ischemia-Reperfusion and Renal Medulla Injury

2002 ◽  
Vol 302 (3) ◽  
pp. 861-870 ◽  
Author(s):  
Jean Pierre Faure ◽  
Thierry Hauet ◽  
Zeqiu Han ◽  
Jean Michel Goujon ◽  
Isabelle Petit ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Ischemia Reperfusion ◽  
Renal Medulla ◽  
Cold Ischemia

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

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