Hypoxia reduces the expression and anti-inflammatory effects of peroxisome proliferator-activated receptor-  in human proximal renal tubular cells

Diabetic kidney disease (DKD) appears to be closely related to lipid deposition in kidney. The aim of this study was to determine whether high glucose (HG) exacerbated lipid deposition by increasing CD36 expression via AKT-PPARγ signaling pathway. Our results showed that HG activated AKT signaling pathway, followed by an increase in PPARγ that induced CD36 overexpression, ultimately causing lipid deposition in HK-2 cells. We also found that inhibition of AKT-PPARγ signaling pathway or knockdown of CD36 could reduce HG-induced lipid accumulation in HK-2 cells. These results indicated that AKT-PPARγ signaling pathway mediated HG-induced lipid deposition by upregulating CD36 expression in HK-2 cells and that inhibition of AKT-PPARγ signaling pathway had the potential beneficial effects of reducing lipid deposition in diabetic kidney.

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Faculty Opinions recommendation of Sestrin-2 and BNIP3 regulate autophagy and mitophagy in renal tubular cells in acute kidney injury.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718012810.793481005 ◽

2013 ◽

Author(s):

Kenneth Hallows ◽

Mohammad Al-bataineh

Keyword(s):

Acute Kidney Injury ◽

Kidney Injury ◽

Tubular Cells ◽

Renal Tubular Cells ◽

Renal Tubular

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Radioprotective effect of pioglitazone against genotoxicity induced by ionizing radiation in human healthy lymphocytes

Cardiovascular & Hematological Agents in Medicinal Chemistry ◽

10.2174/1871525718666200525005231 ◽

2020 ◽

Vol 18 ◽

Author(s):

Roya Kazemi ◽

Seyed Jalal Hosseinimehr

Keyword(s):

Ionizing Radiation ◽

Human Lymphocytes ◽

Peroxisome Proliferator ◽

Peroxisome Proliferator Activated Receptor ◽

The Mean ◽

Anti Inflammatory ◽

Sensitizing Effect ◽

Binucleated Lymphocytes ◽

Inflammatory Effect

Objective: Pioglitazone (PG) is used to control high blood sugar in patients with type 2 diabetes mellitus. PG acts as a peroxisome proliferator-activated receptor γ agonist. In addition to the insulin-sensitizing effect, PG possesses anti-inflammatory effect. In this study, the protective effect of PG was evaluated against DNA damage induced by ionizing radiation in human healthy lymphocytes. Methods: The microtubes containing human whole blood were treated with PG at various concentrations (1-50 μM) for three hours. Then, the blood samples were irradiated with X-ray. Lymphocytes were cultured for determining the frequency of micronuclei as a genotoxicity biomarker in binucleated lymphocytes. Results: The mean percentage of micronuclei was significantly increased in human lymphocytes when were exposed to IR, while it was decreased in lymphocytes pre-treated with PG. The maximum reduction in the frequency of micronuclei in irradiated lymphocytes was observed at 5 μM of PG treatment (48% decrease). Conclusion: The anti-inflammatory property is suggested the mechanism action of PG for protection human lymphocytes against genotoxicity induced by ionizing radiation.

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