SP200SERUM 2-CYSTEINE PEROXIREDOXINS: POTENTIAL BIOMARKERS OF IGA NEPHROPATHY AND LUPUS NEPHRITIS

The significance of interstitial inflammatory and chronic tubulointerstitial lesions was studied in relation to the severity of glomerular lesions in 62 patients with lupus nephritis and 88 with IgA nephropathy. Severe interstitial inflammatory and chronic tubulointerstitial lesions were found in patients with severe glomerular lesions in both lupus nephritis and IgA nephropathy. In such cases, the serum creatinine levels at biopsy were high and the renal prognosis was poor regardless of the underlying disease (lupus nephritis or IgA nephropathy). No IgA nephropathy patients with nil or mild glomerular lesions had moderate or severe interstitial inflammatory and/or chronic tubulointerstitial lesions. In contrast, predominantly severe interstitial inflammatory lesions were found in 36% of lupus nephritis patients with nil or mild glomerular lesions. The prevalence of interstitial immune complexes deposition was markedly high in those with predominant interstitial inflammatory lesions. However, the severity of chronic tubulointerstitial lesions was mild and renal function did not deteriorate in the mean follow-up periods of 68.6 months. It is suggested that, besides the tubulointerstitial lesions attributable to the severe concomitant glomerular damage, the interstitial deposition of immune complexes per se plays a pathogenic role in the interstitial inflammatory lesions in lupus nephritis. Its prognostic significance, however, was considered to be minor.

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H.P. ACTHAR® GEL IN PATIENTS WITH IGA NEPHROPATHY, MEMBRANOUS LUPUS NEPHRITIS AND MINIMAL CHANGE DISEASE: A RETROSPECTIVE CASE SERIES

American Journal of Kidney Diseases ◽

10.1053/j.ajkd.2015.02.139 ◽

2015 ◽

Vol 65 (4) ◽

pp. A49 ◽

Cited By ~ 2

Keyword(s):

Lupus Nephritis ◽

Iga Nephropathy ◽

Minimal Change Disease ◽

Case Series ◽

Minimal Change ◽

Retrospective Case ◽

Retrospective Case Series ◽

Membranous Lupus Nephritis

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Interstitial Inflammatory and Chronic Tubulointerstitial Lesions in Lupus Nephritis: Comparison with Those in IgA Nephropathy

Lupus ◽

10.1177/096120339300200410 ◽

1993 ◽

Vol 2 (4) ◽

pp. 261-268 ◽

Cited By ~ 3

Author(s):

Tatsuo Yamamoto ◽

Mitsumasa Nagase ◽

Akira Hishida ◽

Nishio Honda

Keyword(s):

Lupus Nephritis ◽

Iga Nephropathy ◽

Tubulointerstitial Lesions

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Spectrum of Biopsy Proven Glomerular Disease in Children at Kanti Children’s Hospital

Journal of Nepal Paediatric Society ◽

10.3126/jnps.v34i3.11162 ◽

2015 ◽

Vol 34 (3) ◽

pp. 225-229 ◽

Cited By ~ 1

Author(s):

N Khatun ◽

KP Bista ◽

C Mahaseth

Keyword(s):

Nephrotic Syndrome ◽

Lupus Nephritis ◽

Renal Disease ◽

Iga Nephropathy ◽

Kidney Biopsy ◽

Demographic Data ◽

Glomerular Disease ◽

Children's Hospital ◽

Steroid Dependent ◽

Children’S Hospital

Introduction: Glomerular disease is one of the most common forms of renal disease and can have many different clinical presentations. However there is variation in the prevalence in the type of glomerular disease according to geographical location and race of population. The aim of the study was to find the overall distribution pattern of glomerular disease based on renal biopsies. Material and Methods: The medical records of all children who underwent kidney biopsy (n=29) between January 2012-june 2014 were analyzed. In this retrospective study we review children from Kanti Children’s Hospital, Nephrology Department. Demographic data including age, sex and indication of kidney biopsy as well as complication of the procedures were recorded. Result: A total number of thirty one biopsies were done. Two children were excluded from the study due to inadequate tissue and artifacts defects in preservative. The remaining twenty nine biopsies were included in the study and analyzed. Renal disease was found nearly equal in both male and female (51.72% vs. 48.27%).Mean age was 10.95±3.30 years. Maximum number of biopsies (75.86%) was performed between 10- 15 years. The most common indication of the kidney biopsy was nephrotic syndrome (steroid resistant nephrotic syndrome and steroid dependent nephrotic syndrome, 31.02% followed by lupus nephritis 27.58%.The most common glomerular disease were focal segmental glomerulosclerosis and lupus nephritis both contribute 27.58%. Among lupus nephritis class III was found more common (44.44%) in children. IgA nephropathy was also contribute (17.24%) in all glomerular disease. Regarding the kidney biopsy complication gross hematuria was observed in 6.89% of children which was self-limited. Conclusion: FSGS and lupus nephritis is becoming the most common glomerular disease in children attending the Kanti Children Hospital. IgA nephropathy was also contribute the glomerular disease in the children. J Nepal Paediatr Soc 2014;34(3):225-229 DOI: http://dx.doi.org/10.3126/jnps.v34i3.11162

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Weighted Gene Co-expression Network Analysis Reveals Different Immunity but Shared Renal Pathology Between IgA Nephropathy and Lupus Nephritis

Frontiers in Genetics ◽

10.3389/fgene.2021.634171 ◽

2021 ◽

Vol 12 ◽

Author(s):

Ni-Ya Jia ◽

Xing-Zi Liu ◽

Zhao Zhang ◽

Hong Zhang

Keyword(s):

Gene Expression ◽

Cell Death ◽

Network Analysis ◽

Lupus Nephritis ◽

Iga Nephropathy ◽

Enrichment Analysis ◽

Extracellular Vesicle ◽

Gene Modules ◽

Similarities And Differences ◽

Blue Module

Both IgA nephropathy (IgAN) and lupus nephritis (LN) are immunity-related diseases with a complex, polygenic, and pleiotropic genetic architecture. However, the mechanism by which the genetic variants impart immunity or renal dysfunction remains to be clarified. In this study, using gene expression datasets as a quantitative readout of peripheral blood mononuclear cell (PBMC)- and kidney-based molecular phenotypes, we analyzed the similarities and differences in the patterns of gene expression perturbations associated with the systematic and kidney immunity in IgAN and LN. Original gene expression datasets for PBMC, glomerulus, and tubule from IgAN and systemic lupus erythematosus (SLE) patients as well as corresponding controls were obtained from the Gene Expression Omnibus (GEO) database. The similarities and differences in the expression patterns were detected according to gene differential expression. Weighted gene co-expression network analysis (WGCNA) was used to cluster and screen the co-expressed gene modules. The disease correlations were then identified by cell-specific and functional enrichment analyses. By combining these results with the genotype data, we identified the differentially expressed genes causatively associated with the disease. There was a significant positive correlation with the kidney expression profile, but no significant correlation with PBMC. Three co-expression gene modules were screened by WGCNA and enrichment analysis. Among them, blue module was enriched for glomerulus and podocyte (P < 0.05) and positively correlated with both diseases (P < 0.05), mainly via immune regulatory pathways. Pink module and purple module were enriched for tubular epithelium and correlated with both diseases (P < 0.05) through predominant cell death and extracellular vesicle pathways, respectively. In genome-wide association study (GWAS) enrichment analysis, blue module was identified as the high-risk gene module that distinguishes LN from SLE and contains PSMB8 and PSMB9, the susceptibility genes for IgAN. In conclusion, IgAN and LN showed different systematic immunity but similarly abnormal immunity in kidney. Immunological pathways may be involved in the glomerulopathy and cell death together with the extracellular vesicle pathway, which may be involved in the tubular injury in both diseases. Blue module may cover the causal susceptibility gene for IgAN and LN.

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Conventional Type 1 Dendritic Cells (cDC1) in Human Kidney Diseases: Clinico-Pathological Correlations

Frontiers in Immunology ◽

10.3389/fimmu.2021.635212 ◽

2021 ◽

Vol 12 ◽

Author(s):

Titi Chen ◽

Qi Cao ◽

Ruifeng Wang ◽

Guoping Zheng ◽

Farhana Azmi ◽

...

Keyword(s):

T Cells ◽

Lupus Nephritis ◽

Iga Nephropathy ◽

Interstitial Fibrosis ◽

Kidney Diseases ◽

Human Kidney ◽

Acute Interstitial Nephritis ◽

Cell Number ◽

Pathological Features ◽

Cross Presentation

BackgroundcDC1 is a subset of conventional DCs, whose most recognized function is cross-presentation to CD8+ T cells. We conducted this study to investigate the number and location of cDC1s in various human kidney diseases as well as their correlation with clinico-pathological features and CD8+ T cells.MethodsWe analyzed 135 kidney biopsies samples. Kidney diseases included: acute tubular necrosis (ATN), acute interstitial nephritis (AIN), proliferative glomerulonephritis (GN) (IgA nephropathy, lupus nephritis, pauci-immune GN, anti-GBM disease), non-proliferative GN (minimal change disease, membranous nephropathy) and diabetic nephropathy. Indirect immunofluorescence staining was used to quantify cDC1s, CD1c+ DCs, and CD8+ T cells.ResultscDC1s were rarely present in normal kidneys. Their number increased significantly in ATN and proliferative GN, proportionally much more than CD1c+ DCs. cDC1s were mainly found in the interstitium, except in lupus nephritis, pauci-immune GN and anti-GBM disease, where they were prominent in glomeruli and peri-glomerular regions. The number of cDC1s correlated with disease severity in ATN, number of crescents in pauci-immune GN, interstitial fibrosis in IgA nephropathy and lupus nephritis, as well as prognosis in IgA nephropathy. The number of CD8+ T cells also increased significantly in these conditions and cDC1 number correlated with CD8+ T cell number in lupus nephritis and pauci-immune GN, with many of them closely co-localized.ConclusionscDC1 number correlated with various clinic-pathological features and prognosis reflecting a possible role in these conditions. Their association with CD8+ T cells suggests a combined mechanism in keeping with the results in animal models.

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Peroxiredoxins as Markers of Oxidative Stress in IgA Nephropathy, Membranous Nephropathy and Lupus Nephritis

Archivum Immunologiae et Therapiae Experimentalis ◽

10.1007/s00005-021-00638-1 ◽

2021 ◽

Vol 70 (1) ◽

Author(s):

Natalia Krata ◽

Bartosz Foroncewicz ◽

Radosław Zagożdżon ◽

Barbara Moszczuk ◽

Magdalena Zielenkiewicz ◽

...

Keyword(s):

Oxidative Stress ◽

Lupus Nephritis ◽

Iga Nephropathy ◽

Membranous Nephropathy ◽

Diagnostic Procedures ◽

Renal Diseases ◽

Serum Concentrations ◽

Immune Mediated ◽

Markers Of Oxidative Stress ◽

Estimated Glomerular Filtration Rates

AbstractIgA nephropathy (IgAN), membranous nephropathy (MN), and lupus nephritis (LN) represent important causes of chronic kidney disease. They belong to the immune-mediated glomerulonephritis (GNs), and have distinct pathogenesis, distinct clinical courses, and variable responses to treatment. Therefore, specific diagnostic procedures are necessary for more effective patient management. Recently, a role for oxidative stress has been proposed in various renal disorders. Thus, molecules related to oxidative stress, such as 2-Cys-peroxiredoxins (PRDXs), may represent plausible candidates for biomarkers in renal pathologies. The aim of this study was to assess whether there are differences between individual GNs and healthy controls in the context of PRDXs serum concentration. We enrolled 108 patients with biopsy-proven IgAN (47), MN (26), LN (35) and 30 healthy age- and sex-matched controls. The serum concentrations of PRDX 1–5 were measured with ELISA assays and correlated with demographic and clinical data. The PRDXs’ concentration varied depending on the GN type. We also observed an association of PRDXs with lower estimated glomerular filtration rates, complement, hemoglobin, and body mass index. Our study indicates that individual PRDX can play roles in pathophysiology of selected GNs and that their serum concentrations may become useful as a new supplementary diagnostic markers in IgAN, MN as well as LN. The results of this study open a new avenue for prospective research on PRDXs in renal diseases.

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